Coconut embryo inducing medium and method for obtaining in-vitro regeneration plant based on coconut zygotic embryo thin cell layer culture
A technology for inducing culture medium and culture medium, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low reproduction coefficient, separation of traits of hybrid offspring, long growth cycle, etc., and achieve short reproduction cycle, sustainable Good controllable, easy-to-cultivate effects
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[0093] Example 1
[0094] A coconut embryo induction medium, which uses a modified Y3 medium (Y3*) as the basic medium. The modified Y3 medium is obtained by adjusting the content of the following components on the basis of the internationally used Y3 medium: 0.025 mg / L CuSO 4 ·5H 2 O, 56mg / L Na 2 ·EDTA and 12mg / L FeSO 4 ·7H 2 O; At the same time the basic medium also contains 1mg / L dicamba, 1mg / L NAA, a mass concentration of 3% sucrose, a mass concentration of 0.35% agar and a mass concentration of 0.1% activated carbon; the pH of the coconut embryo induction medium The value is 5.5. Prepare and sterilize to obtain coconut embryo induction medium.
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[0095] Example 2
[0096] A coconut embryo induction medium, which uses a modified Y3 medium (Y3*) as the basic medium. The modified Y3 medium is obtained by adjusting the content of the following components on the basis of the internationally accepted Y3 medium: 0.25 mg / L CuSO 4 ·5H 2 O, 25mg / L Na 2 ·EDTA and 45mg / L FeSO 4 ·7H 2 O; At the same time the minimal medium contains 0.3mg / L 2,4D, 0.8mg / L dicamba, 0.3mg / L NAA, mass concentration of 6% sucrose, mass concentration of 0.4% agar and mass concentration of 0.18%~0.23 % Activated carbon, pH value is 5.4. Prepare and sterilize to obtain coconut embryo induction medium.
Example Embodiment
[0097] Example 3
[0098] A coconut embryo induction medium, using modified Y3 medium (Y3*) as the basic medium, which is obtained by adjusting the content of the following components on the basis of the internationally accepted Y3 medium: 0.1 mg / L CuSO 4 ·5H 2 O, 40mg / L Na 2 ·EDTA and 30mg / L FeSO 4 ·7H 2 O; At the same time the basic medium also contains 0.5mg / L 2,4D, 0.5mg / L dicamba, 0.5mg / L NAA, the mass concentration is 4.2% sucrose, the mass concentration is 0.4% agar and the mass concentration is 0.18% activated carbon ; The pH value of the coconut embryo induction medium is 5.6. Prepare and sterilize to obtain coconut embryo induction medium.
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