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Application of Osmanthus fragrans flower extracts in preparation of IDO (indoleamine-2,3-dioxygenase) inhibitors

A kind of sweet-scented osmanthus extract, the technology of inhibitor, is applied in the field of preparation IDO inhibitor of sweet-scented osmanthus extract

Active Publication Date: 2018-11-13
江苏凯吉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For this reason, the technical problem to be solved in the present invention is the problem that no sweet-scented osmanthus extract is used for the preparation of IDO inhibitors in the prior art, thereby providing the purposes of sweet-scented osmanthus extract in the preparation of IDO inhibitors

Method used

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  • Application of Osmanthus fragrans flower extracts in preparation of IDO (indoleamine-2,3-dioxygenase) inhibitors
  • Application of Osmanthus fragrans flower extracts in preparation of IDO (indoleamine-2,3-dioxygenase) inhibitors

Examples

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Effect test

Embodiment 1

[0026] Example 1 Preparation of sweet-scented osmanthus extract

[0027] Get the dried flowers and leaves of Flos Osmanthus fragrans, pulverize, add 15 times the volume concentration of weight and extract 2 times at room temperature with ethanol aqueous solution of 85%, extract 7 days at every turn, combine extracts, concentrate under reduced pressure, get osmanthus extract, continue to reduce Concentrate under pressure and dry to obtain sweet-scented osmanthus extract A.

[0028] Get sweet-scented osmanthus extract and add 15% aqueous ethanol to dissolve, and the supernatant after centrifugation is purified by D101 macroporous resin column chromatography (the diameter of the macroporous resin column is 8cm, and the column volume is 3.5L), using water as mobile phase A. Using ethanol as mobile phase B, carry out gradient elution according to the following procedure (the flow rate of gradient elution is 3BV / h): first use A:B volume ratio of 90%:10% mobile phase to elute 5BV, a...

Embodiment 2

[0029] Example 2 Preparation of sweet-scented osmanthus extract

[0030] Get the leaves of Osmanthus fragrans, pulverize, add 10 times the volume concentration of the volume concentration of 90% ethanol aqueous solution to extract once at room temperature, extract 14 days each time, combine the extracts, concentrate under reduced pressure to obtain osmanthus extract, continue to concentrate under reduced pressure , drying, to obtain osmanthus extract A.

[0031] Get sweet-scented osmanthus extract and add 10% aqueous ethanol to dissolve, and the supernatant after centrifugation is purified by AB-8 macroporous resin column chromatography (the diameter of the macroporous resin column is 8cm, and the column volume is 3.5L). Mobile phase A, using ethanol as mobile phase B, carry out gradient elution according to the following procedures (the flow rate of gradient elution is 3BV / h): first use A:B volume ratio of 90%:10% mobile phase to elute 4BV, Then use A:B volume ratio of 70%...

Embodiment 3

[0032] Example 3 Preparation of sweet-scented osmanthus extract

[0033] Get the dried flower of Osmanthus osmanthus, pulverize, add 20 times the volume concentration of weight and be that the ethanol aqueous solution of 70% is extracted 3 times at room temperature, extract 1 day each time, combine extraction liquid, concentrate under reduced pressure, obtain sweet-scented osmanthus extract, continue to concentrate under reduced pressure , drying, to obtain osmanthus extract A.

[0034] Get sweet-scented osmanthus extract and add 30% aqueous ethanol to dissolve, and the supernatant after centrifugation is purified by AB-8 macroporous resin column chromatography (the diameter of the macroporous resin column is 8cm, and the column volume is 3.5L). Mobile phase A, using ethanol as mobile phase B, carry out gradient elution according to the following procedure (the flow rate of gradient elution is 3BV / h): first use A:B volume ratio of 90%:10% mobile phase to elute 6BV, Then use...

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Abstract

The invention belongs to the field of medicines or health products and particularly relates to application of Osmanthus fragrans flower extracts in the preparation of IDO (indoleamine-2,3-dioxygenase)inhibitors. All Osmanthus fragrans flower extracts A, B and C are of certain IDO inhibitory activity and are suitable for treating Alzheimer's disease, autoimmune diseases, ankylosing spondylitis, bacterial infections, cataract, mood disorder, and depression or anxiety, wherein the Osmanthus fragrans flower extract B has more significant IDO inhibitory activity and is nearly as good in inhibitoractivity against IDO in cells as a positive control, 1-MT (1-methyltryptophan).

Description

technical field [0001] The invention belongs to the field of medicines or health care products, and in particular relates to the use of sweet-scented osmanthus extracts in preparing IDO inhibitors. Background technique [0002] IDO (indoleamine-2, 3-dioxygenase) is the only rate-limiting enzyme that catalyzes the metabolism of tryptophan along the kynurenine pathway (KP) outside the liver. , can decompose tryptophan into various metabolites such as L-kynurenine, picolinic acid and quinolinic acid. L-Tryptophan is an amino acid necessary to maintain cell activation and proliferation in the human body, and is also an indispensable component of protein. Its deficiency will lead to abnormal function of some important cells. Since it was discovered in 1967, the mechanism of IDO inhibiting the proliferation of pathogenic microorganisms by degrading tryptophan, and the relationship between IDO and nervous system diseases have been gradually clarified; and studies have confirmed th...

Claims

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Application Information

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IPC IPC(8): A61K36/63A61P25/28A61P37/02A61P19/08A61P31/04A61P25/24A61P25/22A61P25/00A61P27/12A61K133/00A61K127/00
CPCA61K36/63A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 温尧林
Owner 江苏凯吉生物科技有限公司