Preparation for treating premature ovarian failure and preparation method

A technology for premature ovarian failure and preparation, applied in the field of biological stem cells, can solve the problems of increasing the risk of endometrial cancer, ovarian cancer and breast cancer, replanting cancer cells, and difficult surgery, and achieves fertility improvement and endocrine function recovery. , the effect of long survival

Inactive Publication Date: 2018-11-16
天津欣普赛尔生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, POF treatment methods mainly include: 1) hormone replacement therapy; 2) egg donation / resuscitation egg and embryo transfer in hormone replacement therapy cycle. Both methods require hormone support, but this will increase the risk of endometrial cancer and ovarian cancer. , the risk of breast cancer; 3) Frozen ovary transplantation technology can restore the reproductive and endocrine capabilities of cancer patients, but the operation is difficult and autologous transplantation may cause cancer cell replantation, and the current egg freezing technology is not yet available. Mature, so traditional treatment methods cannot fundamentally solve premature ovarian failure

Method used

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  • Preparation for treating premature ovarian failure and preparation method
  • Preparation for treating premature ovarian failure and preparation method
  • Preparation for treating premature ovarian failure and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] (1) Autologous endometrial mesenchymal stem cell culture: select autologous endometrial mesenchymal stem cells from the cell bank for recovery, and use phenol red-free DMEM / DF12 and 10% fetal bovine serum as the complete medium , T75 cell culture flask at 37 °C, 5% CO 2 Cultivate under the same conditions, and after the cell growth reaches 90%, collect the cell culture supernatant, discard it, trypsinize and recover all the cells before use;

[0028] (2) Preparation of matrix solution: according to the volume fraction ratio, select 1-5 parts of medicinal human serum albumin, 3-5 parts of estrogen and 90-95 parts of compound electrolyte injection, and put them into the mixing tank and stir evenly. Obtain matrix solution for use;

[0029] (3) Preparation of pharmaceutical preparations: according to the ratio of parts by volume, select 95 parts of matrix solution and 5 parts of autologous endometrial mesenchymal stem cell extract and mix them until they are evenly mixed, ...

Embodiment 2

[0031] (1) Autologous endometrial mesenchymal stem cell culture: select autologous endometrial mesenchymal stem cells from the cell bank for recovery, and use phenol red-free DMEM / DF12 and 10% fetal bovine serum as the complete medium , T75 cell culture flask at 37 °C, 5% CO 2 Cultivate under certain conditions, and after the cell growth rate reaches 95%, collect the cell culture supernatant, discard it, trypsinize and recover all the cells before use;

[0032] (2) Preparation of matrix solution: according to the volume fraction ratio, select 1-5 parts of medicinal human serum albumin, 3-5 parts of estrogen and 90-95 parts of compound electrolyte injection, and put them into the mixing tank and stir evenly. Obtain matrix solution for use;

[0033] (3) Preparation of pharmaceutical preparations: according to the ratio of parts by volume, select 95 parts of matrix solution and 5 parts of autologous endometrial mesenchymal stem cell extract and mix them until they are evenly mix...

Embodiment 3

[0035] (1) Autologous endometrial mesenchymal stem cell culture: select autologous endometrial mesenchymal stem cells from the cell bank for recovery, and use phenol red-free DMEM / DF12 and 10% fetal bovine serum as the complete medium , T75 cell culture flask at 37 °C, 5% CO 2 Cultivate under certain conditions, and after the cell growth rate reaches 93%, collect the cell culture supernatant, discard it, trypsinize and recover all the cells before use;

[0036] (2) Preparation of matrix solution: according to the volume fraction ratio, select 1-5 parts of medicinal human serum albumin, 3-5 parts of estrogen and 90-95 parts of compound electrolyte injection, and put them into the mixing tank and stir evenly. Obtain matrix solution for use;

[0037] (3) Preparation of pharmaceutical preparations: according to the ratio of parts by volume, select 95 parts of matrix solution and 5 parts of autologous endometrial mesenchymal stem cell extract and mix them until they are evenly mix...

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Abstract

The invention provides a preparation for treating premature ovarian failure and a preparation method, and relates to the technical field of biological stem cells. The preparation is prepared from autologous endometrial mesenchymal stem cells and a matrix solution, the matrix solution is prepared from compound electrolytes injection, oestrogen and medicinal human blood albumin, the autologous endometrial mesenchymal stem cells are extracted from human endometrial mesenchymal stem cells, the preparation is obtained through culture according to a normal culture method, the preparation survival time is longer, the preparation can be used for repairing an ovary structure, the endocrine function of the ovary is recovered, and the premature ovarian failure is effectively treated.

Description

technical field [0001] The invention relates to the technical field of biological stem cells, in particular to a preparation and a preparation method for treating premature ovarian failure. Background technique [0002] In recent years, the incidence of premature ovarian failure (Premature Ovarian Failure, POF) has been increasing year by year, and there is a trend of younger age. The definition of premature ovarian failure was first proposed by Moraes-Ruehsen and Jones in 1967. It refers to the non-physiological amenorrhea that occurs after puberty and before the age of 40. The symptoms are the same as those of natural menopause, often accompanied by hot flashes, night sweats, Primary and secondary infertility, systemic and genital atrophy are the main features of a disease. Severe POF even affects the quality of life of patients and causes great mental pressure. [0003] At present, POF treatment methods mainly include: 1) hormone replacement therapy; 2) egg donation / resu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P15/00
CPCA61P15/00A61K35/28
Inventor 张冰晶鲁振宇洪敬欣
Owner 天津欣普赛尔生物医药科技有限公司
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