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Kit for simultaneously detecting capecitabine and its metabolite in blood plasma and detection method and application thereof

A technology for capecitabine and its metabolites, which is applied in the field of kits for the simultaneous detection of capecitabine and its metabolites in plasma, can solve the problems of varying content, large differences in physicochemical properties, and high technical difficulty, and achieve sensitivity High, satisfactory matrix effect, simple pretreatment process

Active Publication Date: 2018-11-16
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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Problems solved by technology

However, due to the large differences in the physical and chemical properties of capecitabine and its metabolites, as well as the different levels in blood, the simultaneous detection technology is difficult. At present, there is no ultra-high performance liquid chromatography tandem mass spectrometry technology for the simultaneous detection of capecitabine in plasma. and its five metabolites reported in the literature

Method used

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  • Kit for simultaneously detecting capecitabine and its metabolite in blood plasma and detection method and application thereof
  • Kit for simultaneously detecting capecitabine and its metabolite in blood plasma and detection method and application thereof
  • Kit for simultaneously detecting capecitabine and its metabolite in blood plasma and detection method and application thereof

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Embodiment 1

[0055] 1. Experimental materials

[0056] The plasma samples used came from the plasma of patients with colorectal cancer who took capecitabine in Shanghai Changzheng Hospital from October to November 2016.

[0057] (1) Instruments. Agilent 6460A triple quadrupole mass spectrometer; Agilent 1290Series ultra-high performance liquid chromatography; adjustable pipettes (Eppendorf, 0.5-10 μL, 10-100 μL, 100-1000 μL); one hundred thousandth balance (Sartorius company, CPA255D type); Vortex mixer (Labnet Company, VX-200 type); Centrifuge (Beili Company, DT-5 type); Freeze drying apparatus (Gene Company, Scanspeed Mari Vac Alpha type).

[0058] (2) Reagent consumables. Chromatographically pure methanol and analytically pure acetonitrile (Merck); chromatographically pure formic acid (Tiandi); double distilled water (Watsons); ethyl acetate (Caledon); isopropanol (Thermo Fisher).

[0059] (3) The reference substance of the compound to be tested and the internal standard reference su...

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Abstract

The invention discloses a kit for simultaneously detecting capecitabine and its metabolite in blood plasma. The capecitabine and its metabolite are respectively capecitabine, 5'-deoxy-5-gemcitabine, 5'-deoxy-5-floxuridine, 2'-deoxy-5-floxuridine, 5-fluorouracil, and 5-fluoro-5,6-dihydrouracil; the kit also comprises the following reagents: an eluate, contrast mother liquor, a mixed internal standard solution, a weak solution, an extract, and quality control liquid. The invention also discloses an application of the kit for simultaneously detecting capecitabine and its metabolite in blood plasma in preparation of a kit for diagnosing or screening hematology of malignant tumors. The kit has the advantages of high sensitivity, high specificity, and high accuracy for simultaneously detecting capecitabine and its metabolite in blood plasma, and a pre-processing process is simple.

Description

technical field [0001] The invention belongs to the technical field of blood quantitative detection, and specifically relates to a kit for simultaneously detecting capecitabine and its metabolites in blood plasma, a detection method and an application thereof. Background technique [0002] Since the early 1990s, cancer has become the number one cause of death in my country, among which malignant tumors of the digestive tract occupy the forefront of the morbidity and mortality of all malignant tumors, seriously endangering people's health and life [item] Yong Bing. The prevalence and current situation of malignant tumors of the digestive tract. Tumors, 2002,22(4):336-339.]. According to statistics, in 2013, there were about 1.05 million new patients with digestive tract malignant tumors nationwide, accounting for about 30% of the total incidence of malignant tumors, including about 420,000 new gastric cancer patients, 350,000 new colorectal cancer patients, 280,000 patients; ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/045
Inventor 王志鹏高守红杨阳陈万生恽芸蕾张凤李明明仲人前
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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