Detection card for detecting benzodiazepine substance in health care products and preparation method thereof

A benzodiazepine and detection card technology, applied in the field of immune colloidal gold detection card, can solve the problems of complex operation and slow detection speed, and achieve the effect of simple operation, fast detection and high specificity

Active Publication Date: 2018-11-16
杭州泰熙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcomings of slow detection speed and complicated operation in the prior art, the present invention provides a fast, convenient and high-sensitivity detection card for detecting benzodiazepines in health care products

Method used

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  • Detection card for detecting benzodiazepine substance in health care products and preparation method thereof
  • Detection card for detecting benzodiazepine substance in health care products and preparation method thereof
  • Detection card for detecting benzodiazepine substance in health care products and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A detection card for detecting benzodiazepines in health care products, such as figure 1 As shown, including detection test strips, detection reagent strips starting from sample pad 1 are gold-labeled binding pad 2, nitrocellulose membrane 3, water-absorbing pad 4, and gold-labeled binding pad 2 is an anti-benzene film coated with colloidal gold. The glass fiber gasket of diazepam monoclonal antibody, the nitrocellulose membrane 3 is a nitrocellulose membrane provided with a detection line 5 and a quality control line 6, and the detection line 5 is coated with benzodiazepine-bovine serum albumin Complex, quality control line 6 coated with goat anti-mouse IgG. The pore size of the nitrocellulose membrane 3 is 3 μm.

Embodiment 2

[0031] Same as Example 1, the difference is that the membrane pore size of the nitrocellulose membrane 3 in Example 2 is 4 μm.

Embodiment 3

[0033] Same as Example 1, the difference is that the membrane pore size of the nitrocellulose membrane 3 in Example 3 is 5 μm.

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Abstract

The invention relates to the technical field of an immunocolloid gold detection card, and discloses a detection card for detecting a benzodiazepine substance in health care products. The detection card includes a detection test strip, and the detection test strip is a colloidal-gold combination pad (2), a nitrocellulose membrane (3), and an absorbent pad (4) in order from a sample pad (1); the colloidal-gold combination pad (2) is a glass fiber gasket coated with a colloidal gold-labeled anti-benzodiazepine monoclonal antibody, the nitrocellulose membrane (3) is the nitrocellulose membrane provided with a detection line (5) and a quality control line (6), the detection line (5) is coated with a benzodiazepine-bovine serum albumin complex, and the quality control line (6) is coated with goat anti-mouse IgG. The invention also discloses a preparation method of the detection card for detecting the benzodiazepine substance in the health care products. The detection card can avoid the trouble of using complex identifiers or tracing buffer solutions, reduces sample differences, and improves sensitivity.

Description

technical field [0001] The invention relates to the technical field of immune colloidal gold detection cards, in particular to a detection card for detecting benzodiazepines in health care products and a preparation method thereof. Background technique [0002] Benzodiazepines belong to the second generation of hypnotics and sedatives, which have various pharmacological effects such as hypnosis, sedation, and antipsychotics. In a tense and competitive society, lack of sleep or sleep disorders have seriously affected the lives of modern people. Benzodiazepines are the most widely used drugs in the field of anti-anxiety and insomnia. Criminals often illegally add benzodiazepines to sedative and hypnotic health foods, and advertise "pure Chinese medicine preparations" to deceive consumers and seek Windfall profits. Long-term use of this drug can lead to tolerance and dependence, whether it is a single abuse or long-term use, it may cause acute or chronic poisoning, especially ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/558G01N33/6854
Inventor 周斌王树明
Owner 杭州泰熙生物技术有限公司
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