Microbial cell exchanging method and optical tweezer device thereof

Abstract

The invention discloses a microbial cell exchanging method and an optical tweezer device thereof. The optical tweezer device comprises a cell culture room, an electric stage, a microscopic objective,an illuminating light source, an image sensor, a first optical tweezer transmitter and a second optical tweezer transmitter, wherein the cell culture room is arranged on the electric stage; the microscopic objective is arranged above the cell culture room; the image sensor is arranged above the microscopic objective; the image sensor is connected with an image display through an image processor; light beam splitters are arranged between the image sensor and the microscopic objective; and the light beam splitters refract laser beams, emitted by the first optical tweezer transmitter and the second optical tweezer transmitter on one side of the microscopic objective, into the cell culture room on the electric stage. Through the microbial cell exchanging method and the optical tweezer device thereof, optical tweezers are used for cell exchange, so that not only the precision is high and cells are unlikely to be damaged, but also growth of the exchanged cells is facilitated and the culturetime is shortened; and the microbial cell exchanging method and the optical tweezer device thereof are widely applied to cell capture and analysis.

Description

technical field [0001] The invention relates to the field of biological cells, in particular to a microbial cell exchange method and an optical tweezers device. Background technique [0002] Microorganisms include: bacteria, viruses, fungi, and some small protists, microscopic algae, etc., which are small and closely related to humans. Covering many types of beneficial and harmful, widely involved in food, medicine, industry and agriculture, environmental protection and many other fields. In textbooks in mainland China, microorganisms are divided into the following eight categories: bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, and spirochetes. Some microorganisms are visible to the naked eye, such as mushrooms and ganoderma that belong to fungi. There are also microorganisms, which are a class of "non-cellular organisms" composed of a few components such as nucleic acids and proteins, but their survival must depend on living cells. [0003] ...

AI Technical Summary

Problems solved by technology

For manual and semi-automatic optical tweezers, it often takes a lot of time to capture the microspheres, which greatly reduces the experimental efficiency and increases the experimental burden on the operator.

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