Intestinal flora kit for diagnosing cerebral infarction and application thereof

An intestinal flora and kit technology, applied in the field of clinical medicine, can solve the problems of intestinal flora kits without cerebral infarction, and achieve the effect of great social significance and economic benefits

Inactive Publication Date: 2018-11-20
天津市环湖医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no gut microbiota kit for diagn

Method used

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Embodiment Construction

[0014] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below in conjunction with the best embodiments.

[0015] As shown, an intestinal flora kit for diagnosing cerebral infarction of the present invention is characterized in that it comprises the following reagents arranged in sequence:

[0016] The first reagent is a water-soluble primer with gene sequences 5’-AGAGTTTGATCATGGCTC-3’ and 5’-CCGTCAATTCCTTTGAGTTT-3’;

[0017] The second reagent is the pGEM-T easy vector plasmid.

[0018] An application of an intestinal flora kit for diagnosing cerebral infarction, which is characterized in:

[0019] a. Using the genomic DNA of the stool specimen as a template, add the first reagent to the water-soluble primers with the gene sequence 5’-AGAGTTTGATCATGGCTC-3’ and 5’-CCGTCAATTCCTTTGAGTTT-3’ to High-Fidelity PCR Master Mix with GC Buffer and high-efficiency high-fideli...

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Abstract

The invention relates to the field of clinical medicine, in particular to an intestinal flora kit for diagnosing cerebral infarction and application thereof. A first reagent is a water-soluble primerwith a gene sequence of 5'-AGAGTTTGATCATGGCTC-3' and 5'-CCGTCAATTCCTTTGAGTTT-3'; a second reagent is a pGEM-T easy vector plasmid; through PCR (polymerase chain reaction)amplification of metabolic specimens, the vector plasmid pGEM-T easy, and transferring into DH5 alpha competent cells, and by exploring an intestinal flora structure of patients with cerebral infarction, the kit provides a new basis for the diagnosis of cerebral infarction, provides possible indications for prevention of cerebral infarction, and has greater social significance and economic benefits.

Description

Technical field [0001] The present invention relates to the field of clinical medicine, in particular to an intestinal flora kit for diagnosing cerebral infarction and its application. Background technique [0002] Cerebral infarction, also known as acute ischemic stroke (Cerebral Ischemia, CI), is a relatively common disease in the elderly, which seriously affects the quality of life of patients. In recent years, due to unreasonable personal living habits or environmental factors in personal life, the age of the first onset of cerebrovascular disease has gradually become younger. Cerebrovascular disease has now become China's first leading disability and second leading cause of death. Although the treatment level of cerebral infarction has been greatly improved in recent years, the treatment effect of most patients is still not ideal, the mortality and disability rate are high, and the prognosis is poor. Therefore, exploring the pathogenesis and prevention of cerebral infarcti...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6869C12Q1/10C12Q1/06C12R1/185
CPCC12Q1/6869C12Q1/689C12Q2531/113
Inventor 朱彧岳伟相蕾阚鹏程程乾胜杨萍
Owner 天津市环湖医院
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