Fluorescent detection system suitable for allergic reaction tester and measuring method thereof
A fluorescence detection and allergic reaction technology, applied in the field of fluorescence detection system, to achieve the effect of expanding the measurement range, reducing volume and power consumption, and good disturbance suppression ability
Pending Publication Date: 2018-11-20
SOUTH CHINA UNIV OF TECH
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At present, there is no mature allergic reaction detection instrument in China. A fluorescent detection system suitable for automatic allergic reaction tester is invented. During the measurement process, a certain light intensity is generated to excite light, detect emitted light intensity, and convert data for large-scale measurement. , and according to a certain communication protocol to transmit data with the host computer and receive commands and parameter configurations from the host computer. In order to ensure the measurement accuracy, in addition to the overall anti-interference plan, it is also necessary to carefully design the measurement circuit and power supply circuit.
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[0039] The specific implementation of the present invention will be described in detail below in conjunction with the accompanying drawings. The structure of the fluorescent detection system applicable to the allergic reaction detector of the present invention is as follows: figure 1 As shown, the fluorescence detection system includes an optical subsystem and an electrical subsystem. The optical subsystem includes an ultraviolet laser light source, an excitation light filter, a measurement sample flow cell, an emission light filter and a photomultiplier tube connected in sequence. The ultraviolet light emitted by the excitation light source will contain a certain frequency band of ultraviolet wavelength components, which can reduce the ultraviolet bandwidth after passing through the excitation light filter; the composition in the sample is complicated, and the emitted light after the fluorescence reaction is also a light wave mixed with various impurities. After emitting the...
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The invention relates to a fluorescent detection system suitable for an allergic reaction tester and a measuring method thereof. The fluorescent detection system comprises an optical sub system and anelectric sub system. The electric sub system comprises a power supply circuit and a photo-electric measuring sub system. The photo-electric measuring sub system comprises an ultraviolet exciting light intensity adjusting module, a photo current amplifying module, an MCU circuit and an RS485 communication module. The photo current amplifying module comprises a current and voltage conversion and signal amplification circuit, a high pass filter, a comparator circuit, a low pass filter and an ADC circuit. A fluorescent signal after the allergic reaction is amplified by the current and voltage conversion and signal amplification circuit and is divided into two signals, one of which enters the low pass filter and the ADC circuit while the other of which enters the high pass filter and the comparator circuit, so that large range continuous measurement of fluorescence is achieved.
Description
technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a fluorescence detection system suitable for an allergic reaction tester and a measurement method thereof. Background technique [0002] Allergic diseases are the most widely affected clinical immune diseases, and are listed by WHO as one of the four major non-infectious diseases (cardiovascular, tumor, obesity, allergy) in the 21st century. Allergies are still an important cause of major diseases such as asthma. The root cause of the disease. Therefore, it is of great significance to strengthen the diagnostic and therapeutic instruments for allergic diseases. [0003] In the allergic reaction test, it is necessary to take the blood sample of the person being tested to cultivate the allergen measurement reagent, and react with different types of allergens, and determine the degree of allergic reaction by detecting the content of histamine in the product. During the ...
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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 李向阳许铭杰
Owner SOUTH CHINA UNIV OF TECH