Method for extracting melanin from rapeseed meal and application of precipitates obtained by method
A technology for melanin and sediment, which is applied in the application, chemical instruments and methods, animal feed, etc., can solve the problems of low extraction rate of melanin, and achieve the effect of improving the purity and the extraction rate.
Active Publication Date: 2018-11-23
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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[0006] In order to solve the problem of low extraction rate of melanin extracted from rapeseed meal in the prior art, an embodiment of the present invention provides a method for extracting melanin from rapeseed meal and the application of the precipitate obtained by the method
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[0027] In order to make the purpose, technical solution and advantages of the present invention clearer, the implementation manners of the present invention will be further described in detail below.
[0028] The embodiment of the present invention provides a method for extracting melanin from rapeseed meal, the method is specifically:
[0029] Weigh 500g rapeseed meal, according to 10 6 ~10 7 Add white rot fungus or Aspergillus oryzae to rapeseed meal at an inoculum amount of spores / g, and ferment for 72 hours at room temperature (25°C) to obtain a fermentation product, which is dried and crushed to obtain a product to be extracted.
[0030] Use a mixed extract of ethanol, hydrochloric acid and water to extract the product to be extracted, the solid-liquid ratio of the product to be extracted to the mixed extract is 1: (4-10), and the final concentration of hydrochloric acid in the mixed extract is 0.5-1mol / L, the volume fraction of ethanol in the mixed extract is 60-80%. ...
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The invention discloses a method for extracting melanin from rapeseed meal and application of precipitates obtained by the method, and belongs to the field of melanin extraction. The method comprisesthe following steps of adding white-rot fungi or aspergillus oryzae into the rapeseed meal for fermentation to obtain a fermentation product; the fermentation product is dried and crushed to obtain aproduct to be extracted; mixed extraction liquid of ethyl alcohol, hydrochloric acid and water is used for leaching the product to be extracted; the material liquid ratio of the product to be extracted to the mixed extraction liquid is 1:(4 to 10); the final concentration of hydrochloric acid is 0.5 to 1mol / L; the volume fraction of ethyl alcohol is 60 to 80 percent; after the leaching, a leachedproduct is obtained; the leached product is centrifuged to obtain supernate and precipitates; the supernate is purified to obtain the melanin. The method has the advantages that the melanin can be separated from substances such as protein and polysaccharide, so that the melanin extraction rate is greatly improved.
Description
technical field [0001] The invention relates to the field of pigment extraction, in particular to a method for extracting melanin from rapeseed meal and an application of the precipitate obtained by the method. Background technique [0002] Melanin is composed of indoles or polyphenols. Studies have shown that melanin has biological activities such as photoprotection of biological pesticides, ultraviolet absorption, free radical scavenging, anti-virus and anti-tumor. Melanin can be divided into natural melanin and synthetic melanin according to the production route. Compared with synthetic melanin, natural melanin has less side effects and higher safety. It is widely used in the coloring of beverages, candies, alcohol and other foods and in the cosmetics industry. Wide application prospects and development potential. [0003] As the main source of natural melanin, plant melanin mainly exists in the epidermal tissue of seeds and fruits. Rapeseed meal is the residue after o...
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Login to View More IPC IPC(8): C09B61/00C09B67/54A23K10/12A23K10/37A23K20/179
CPCA23K10/12A23K10/37A23K20/179C09B61/00C09B67/0096Y02P60/87
Inventor 陈文超马旭丽万霞黄凤洪李旋
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI