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A kind of folic acid compound and its preparation method and application

A compound and folic acid technology, which is applied in the preparation, sampling, and instrumentation of test samples, can solve problems such as the need to improve the sensitivity of test results and the limited amount of methylene blue, and achieve the effect of improving detection sensitivity and ensuring stable dyeing

Active Publication Date: 2021-03-23
辽宁钰航生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above inventions all use the folic acid targeting effect combined with methylene blue staining technology to identify tumor cells, but from the content of the invention, it is simply to screen the type of folic acid, or to strengthen the redox of methylene blue by means process
However, even if the affinity and targeting of folic acid are greatly improved, the amount of methylene blue entering the tumor cells is still limited, so the sensitivity of the detection results still needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment provides a folic acid complex, which comprises the following components calculated by weight percentage: 0.5 wt% folic acid, 0.5 wt% methylene blue, 5 wt% polyvinylpyrrolidone, and 94 wt% ethanol.

[0027] The preparation method of the folic acid complex comprises the following steps: (1) adding the prescribed amount of polyvinylpyrrolidone into ethanol, stirring evenly, adding the prescribed amount of methylene blue, reacting at 30°C for 4 hours, until the methylene blue is completely After polymerizing with polyvinylpyrrolidone, add the prescribed amount of folic acid and continue to react for 2 hours.

[0028] The present embodiment also provides a staining agent for epithelial tissue lesion cells containing folic acid complex, and its composition includes the following components by weight percentage: 9.5wt% of folic acid complex, 5wt% of ascorbic acid, 5wt% of sodium bisulfite, 0.5wt of acetic acid %, deionized water 10wt%, dimethyl sulfoxide 70wt%....

Embodiment 2

[0030] This embodiment provides a folic acid complex, the composition of which is calculated by weight percentage and includes the following components: 3 wt% of folic acid, 1 wt% of methylene blue, 10 wt% of β-cyclodextrin, and 86 wt% of dimethyl sulfoxide. The preparation method is as follows: add the prescribed amount of β-cyclodextrin into the prescribed amount of dimethyl sulfoxide, stir evenly, add methylene blue, and react at 40°C for 3 hours; wait until the methylene blue is completely combined with the β-cyclodextrin After polymerization, add the prescribed amount of folic acid and continue to react for 3 hours.

[0031] This embodiment also provides a staining agent for epithelial tissue lesion cells containing a folic acid complex, and its composition includes the following components by weight percentage: 15wt% of a folic acid complex, 5wt% of ascorbic acid, 5wt% of sodium bisulfite, and 0.7wt% of acetic acid , deionized water 20wt%, dimethyl sulfoxide 54.3wt%. Th...

Embodiment 3

[0042] This embodiment provides a folic acid complex, the composition of which is calculated by weight percentage and includes the following components: 0.05wt% folic acid, 0.05wt% methylene blue, 0.9wt% chitosan, and 99wt% absolute ethanol. The preparation method is as follows: add the prescribed amount of chitosan into the prescribed amount of absolute ethanol, stir evenly, add methylene blue, stir and react at 30°C for 2 hours; after the methylene blue is completely polymerized with the chitosan, Add the prescribed amount of folic acid and continue to react for 3 hours.

[0043] This embodiment also provides a staining agent for epithelial tissue lesion cells containing a folic acid complex, and its composition includes the following components by weight percentage: 1 wt% of a folic acid complex solution, 8.99 wt% of ascorbic acid, 0.01 wt% of acetic acid, and 90 wt% of deionized water %. The preparation method is as follows: successively add ascorbic acid into deionized w...

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Abstract

The invention discloses a folic acid compound as well as a preparation method and application thereof. The folic acid compound comprises the following components by weight percent: 0.05 to 5 percent of folic acid, 0.05 to 5 percent of methylene blue, 0.5 to 20 percent of polymer material, and 75 to 99 percent of solvent. The preparation method of the folic acid compound comprises the following step of continuously stirring the folic acid, the methylene blue and the polymer material solution to react for 2 to 6 hours at 30 to 50 DEG C, thus obtaining the folic acid compound. The folic acid compound is used for preparing epithelial tissue diseased cell staining solution, and the staining solution is used for the primary diagnosis before the pathological analysis of the human body epithelialtissues. The folic acid compound provided in the invention is to improve the solubility of the folic acid and the biological permeability of the methylene blue, so that the concentration of the folicacid and the methylene blue in the epithelial tissue, and the detection sensitivity and accuracy of the epithelial tissue diseased cell staining solution comprising the folic acid compound can be greatly improved.

Description

technical field [0001] The invention relates to a folic acid compound and its preparation method and application. Background technique [0002] With the accelerated pace of life and increased work pressure, the morbidity and mortality of tumors are increasing year by year. The prevention and treatment of tumor diseases has become the focus of research by medical workers. At present, tumor cell screening, including biopsy, exfoliated cell examination and tumor tissue biopsy, has the most direct biological and histological significance for tumor diagnosis. [0003] Methylene blue is a non-toxic biological dyeing material. The oxidized form of the material is blue, and the reduced form is five colors. Under the weak acid condition in the tumor cells, the reduced methylene blue can be oxidized into the oxidized form by the free radicals with strong oxidative properties in the tumor cells, which turns blue, so that it can be preliminarily judged whether there are tumor cells in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 邸航
Owner 辽宁钰航生物医疗科技有限公司