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A method for supercritical extraction of squid active protein high f-value oligopeptides

An active protein and supercritical technology, applied in the field of health food processing, can solve the problems of ineffective degreasing and deodorization, low yield of high F value oligopeptides, low hydrolysis rate, etc., to achieve broad market and application prospects, low osmotic pressure, The effect of low antigenicity

Active Publication Date: 2021-04-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the raw materials used to obtain active high-F value oligopeptides are mainly plant proteins and milk-derived proteins. Using aquatic products as raw materials to prepare high-F-value oligopeptides has low yield and low hydrolysis rate, and cannot effectively degrease. , deodorization, affect the taste

Method used

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  • A method for supercritical extraction of squid active protein high f-value oligopeptides
  • A method for supercritical extraction of squid active protein high f-value oligopeptides

Examples

Experimental program
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Effect test

Embodiment 1

[0033]A supercritical extraction of squid active protein high F value low polypeptide, including the following steps:

[0034](1) Squid pretreatment: remove the squid in dirty to get the squid board, then cut the squid board, crush the treatment, be a block, rinse with deionized water 3 times, becomes a squid, the squid is living South Pacific squid;

[0035](2) Supercritical extraction: extract and remove lipids and astringent substances in squid fish in supercritical carbon dioxide fluids, and extract the squid lysol protein with ethanol, squid lysol 蛋 无 无 无 无The material content is less than 0.2%; the extract is 10 MPa, the temperature is 40 ° C, the time is 0.5 h, the flow rate of supercritical carbon dioxide fluid is 15 l / h, ethanol extraction, ethanol concentration is 90%, squid fish The mass ratio of ethanol is 1: 15, the extraction temperature is 55 ° C, the pH is 9.0;

[0036](3) Enzymatic hydrolysis: The essence of the squid alcohol protein is enzymatically hydrolyzed by two-step...

Embodiment 2

[0039]A supercritical extraction of squid active protein high F value low polypeptide, including the following steps:

[0040](1) Squid pretreatment: remove the squid in dirty to get the squid plate, then cut the squid board, crush the treatment, be a block, rinse with deionized water 4 times, to become a squid fish, squid is living Argentine squid;

[0041](2) Supercritical extraction: extract and remove lipids and astringent substances in squid fish in supercritical carbon dioxide fluids, and extract the squid lysol protein with ethanol, squid lysol 蛋 无 无 无 无The material content is less than 0.2%; the extract is 45 MPa, the temperature is 35 ° C, the time is 2 h, the flow rate of the supercritical carbon dioxide fluid is 40 l / h, and the ethanol is 60%, the squid squid is The mass ratio of ethanol is 1: 8, the extraction temperature is 50 ° C, the pH is 8.0;

[0042](3) Enzymatic hydrolysis: The essence of the squid protein is enzymatic, the first step is ultrasonic enzymatic solution, an...

Embodiment 3

[0046]A supercritical extraction of squid active protein high F value low polypeptide, including the following steps:

[0047](1) Squid pretreatment: remove the squid in dirty to get the squid plate, then cut the squid board, crush the treatment, be a block, rinsed with deionized water 3 times, to be a squid fish; where the squid is free. More than 15 days of Argentine squid;

[0048](2) Supercritical extraction: extract and remove lipids and astringent substances in squid fish in supercritical carbon dioxide fluids, and extract the squid lysol protein with ethanol, squid lysol 蛋 无 无 无 无The material content is less than 0.2%; the extract is 30 MPa, the temperature is 60 ° C, the time is 3.0 h, the flow rate of supercritical carbon dioxide fluid is 40 l / h, ethanol extract, ethanol concentration is 75%, squid fish The mass ratio of ethanol is 1:10, the extraction temperature is 60 ° C, the pH is 8.0;

[0049](3) Enzymatic hydrolysis: The essence of squid protein is enzymatic, the first step ...

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Abstract

The invention belongs to the technical field of health food processing. The invention discloses a method for supercritically extracting squid active protein high F value oligopeptides, which comprises the steps of squid pretreatment, supercritical extraction, enzymolysis, purification and powder making. In the present invention, the supercritical carbon dioxide fluid extraction technology can effectively remove the lipids and smelly and miscellaneous substances in squid protein, and obtain active low-fat squid protein. At the same time, two-step ultrasonic assisted enzymatic hydrolysis fundamentally ensures high The quality of the F-value oligopeptide, the synergy between the various steps and parameters of the method of the present invention can ensure the quality of the high-F-value oligopeptide product; the high-F-value oligopeptide obtained by the present invention is a small molecular short peptide, Compared with free amino acids, it has the advantages of low osmotic pressure and low antigenicity, and is easier to be absorbed and utilized. It can be used as a nutritional supplement for athletes, and has significant effects in liver protection, hepatic encephalopathy, and phenylketonuria treatment.

Description

Technical field[0001]The present invention relates to the field of health food processing, in particular, relating to a method of supercritical extract of squid active protein height f value low polypeptide.Background technique[0002]Squid (Squid) is a soft animal door, the individual is large, the body is long, the meat is tender and juicy, the meat flavor is approximately abalone, the price is low, and nearly 80% of the food. According to reports, squid is one of my country's major aquatic processing raw materials, with an annual processing volume of about 30 to 400,000 tons. However, the current squid is lacking, but not only the raw material utilization is low, but also pollutes the environment. High F value oligopeptide (F value>20) is a small peptide mixture composed of 3 to 9 amino acid residues, and a F value refers to branched chain amino acids (leucine, isoleucine, and proline, refermstriction, BCAA) and aromatic amino acids (benzene). The amount ratio of the substance o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/22
CPCC07K1/145C07K1/22C12P21/06Y02P20/54
Inventor 郭健姜东娇宋文东纪丽丽王亚宁蔡璐
Owner ZHEJIANG OCEAN UNIV
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