A test method for the effect of long-term application of high and low nitrogen fertilizers on corn grain albumin

A test method and albumin technology are applied in the field of experiments on the effect of long-term application of high and low nitrogen fertilizers on maize grain albumin, which can solve the problems that the research on the albumin proteomics of summer maize grains is still blank and so on.

Active Publication Date: 2020-08-07
QINGDAO AGRI UNIV
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Problems solved by technology

However, the proteomics research on albumin in summer maize grains under the condition of long-term targeted fertilization is still blank

Method used

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  • A test method for the effect of long-term application of high and low nitrogen fertilizers on corn grain albumin
  • A test method for the effect of long-term application of high and low nitrogen fertilizers on corn grain albumin
  • A test method for the effect of long-term application of high and low nitrogen fertilizers on corn grain albumin

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Embodiment Construction

[0028] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] 1 Materials and methods

[0030] 1.1 Experimental design

[0031] The experiment started in 1978 at the Laiyang Experimental Station of Qingdao Agricultural University. The soil for testing is non-calcareous fluvo-aquic soil, developed in alluvial parent material, topsoil texture is light loam, organic matter content is 4.10g / kg, total nitrogen content is 0.50g / kg, total phosphorus (P 2 o 5 ) amount is 0.46g / kg, soil available phosphorus (P 2 o 5 ) is 15mg / kg, soil available potassium (K 2 O) is 38mg / kg, and the soil cation replacement capacity is 11.80cmol / kg. A total of 3 treatments (control group, low nitrogen, high nitrogen) were set up in the experiment, and each treatment was repeated 3 times. The experimental site implements a winter wheat-summer corn rotation system, with two crops per ...

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Abstract

The invention discloses a method used for measuring influences of long term applications of high and low nitrogen fertilizers on corn kernel albumin. According to the method, proteomic method is adopted to study long term positioned fertilization of high and low nitrogen fertilizers on summer corn Luyu 16 kernel albumin. It is shown by results that: the albumin content of a reference group is higher than those of two processed groups, and obvious difference strips are observed in quantificative unidirectional SDS-PAGE. It is shown by dimensional electrophoresis results that when a long term low nitrogen treated group is compared with the reference group, 7 differential protein spots are detected, one up-regulation is detected, and 6 down-regulation is detected; when a long term high nitrogen treated group is compared with the reference group, 6 differential protein spots are detected, one up-regulation is detected, and 5 down-regulation is detected. Mass spectrum identification and function classification are adopted, it is found that the proteins are a protein and amino acid metabolism associated protein, an energy metabolism associated protein, an expression regulation and control and defense and stress resistance associated protein, a cell metabolism associated protein, and a protein with unknown functions.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a test method for the effect of long-term application of high and low nitrogen fertilizers on corn grain albumin. Background technique [0002] With the continuous improvement of people's living standards, the requirements for the quality of crop products are getting higher and higher, and the quality of agricultural products is directly related to external environmental factors, such as temperature, light, nutrients, water and soil. Fertilizer is an important part of modern agriculture. The main supply source of nutrients in production directly participates in the coordination of nutrient metabolism and circulation, which inevitably greatly restricts the yield and quality of crops. In recent years, there have been many studies on the effect of nitrogen fertilizer on corn grain protein. Most of these studies are the change of grain protein content and the results ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N27/62G01N21/31G01N1/28G01N1/38
CPCG01N1/286G01N1/38G01N21/31G01N27/44773G01N27/62G01N2001/2866
Inventor 裴玉贺宋希云吴倩郭新梅赵美爱李军刘树堂
Owner QINGDAO AGRI UNIV
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