Inflammatory marker POCT joint detection kit

A combined detection and marker technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of limited application, achieve the effect of simple detection operation, reduce pain and the risk of cross-infection

Inactive Publication Date: 2018-11-30
河南省生物工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is a patent for a fluorescent immunochromatography kit for the joint detection of CRP and SAA (application number: 201521063805.7), but if CRP and SAA are quantitatively detected at the same time, a matching fluorescent immunoassay analyzer is required, and a fluorescent immunoassay analyzer also costs tens of thousands Yuan, which limits its application in family

Method used

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  • Inflammatory marker POCT joint detection kit
  • Inflammatory marker POCT joint detection kit
  • Inflammatory marker POCT joint detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of detection card

[0032] 1. Preparation of CRP and SAA antibody-fluorescent microsphere complexes

[0033] Take 10 μL of Eu-carboxyl fluorescent microspheres and add 400 μL of 0.05M boric acid buffer solution with pH=7.6, mix well; add 40 μL of 1 mg / ml EDC activator and 10 μL of 1 mg / ml NHS solution, shake and activate on a constant temperature oscillator for 20 minutes; Centrifuge at 5000rpm for 20min, discard the supernatant, add 500μL 0.05M pH=7.6 boric acid buffer to redissolve the precipitate, vortex mix, add 40ug CRP and SAA respectively, vortex mix, put in a shaker and oscillate at low speed for 2h; at 5000rpm Centrifuge for 15 min, discard the supernatant, add 1500 μL reconstitution solution to redissolve, and then block with 10 μL blocking solution (20% BSA) for 30 min. The composition of the reconstitution solution is 0.05M pH 8.0 Tris-HCl, 0.1% PVP K30, 0.5 %BSA, 3% Trehalose, 1% Triton X-100, 0.05% NaN 3 .

[0034] 2. Coating mo...

Embodiment 2

[0044] Example 2 Preparation of Fluorescence Immunochromatography Color Card

[0045] 1. Gradient fluorescent microsphere dilution

[0046] a. Take 10 μL of the microsphere stock solution with a concentration of 10 mg / mL into a centrifuge tube.

[0047] b. Add 0.05M PBS to dilute into microsphere solutions with concentrations of 12.5μg / mL, 6.25μg / mL, and 3.125μg / mL;

[0048] 2. Draw a line

[0049] a. Take the nitrocellulose film, paste it on the PVC board, place it on the corresponding position of the three-dimensional dot film gold spraying instrument, and set it aside;

[0050] b. Clean the two pipelines of the three-dimensional dot film spray gold instrument with pure water for 3 times;

[0051] c. Adjust the position of the pipeline so that the distance between the two pipelines is 6mm;

[0052] d. The scribing parameters are set as: scribing speed 50mm / s, scribing volume 1μL / cm;

[0053] e. Inhale the prepared microsphere solutions of different concentrations into t...

Embodiment 3

[0063] The detection limit of the SAA and CRP antibody on the detection card of the present invention is determined

[0064] Sample processing: collect blood with the sample collection device provided in this kit.

[0065] Preparation of the test card: the test card prepared in Example 1 was placed at room temperature for 15 minutes before use.

[0066] Dilute SAA and CRP antigens to 2mg / L, 4mg / L, 8mg / L, 16mg / L, 32mg / L, 64mg / L, 128mg / L,

[0067] Use the combined detection SAA and CRP fluorescent immunochromatography kit set established by the present invention for detection, and put it in the detection device to interpret the result after adding the sample for 5 minutes. The results are shown in Table 1:

[0068] Table 1 The detection limit determination results of SAA and CRP antibodies

[0069]

[0070] It can be seen from Table 1 that the test card detects serial samples diluted with serum amyloid A (SAA) and C-reactive protein (CRP) antigens. From the test results, t...

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Abstract

The invention discloses an inflammatory marker POCT joint detection kit. The inflammatory marker POCT joint detection kit comprises a detection card, a colourimetric card, a sample collection apparatus and a detection apparatus, wherein the detection card consists of a test strip and a rectangular card shell; the test strip consists of a bottom plate, a sample mat, a joint mat, a nitrocellulose membrane and a water absorption mat, the sample mat, the joint mat, the nitrocellulose membrane and the water absorption mat are successively assembled onto the bottom plate in a lap joint manner, and the joint mat is fixedly provided with CRP and SAA antibody-fluorescent microbead composite; and the nitrocellulose membrane in a detection area is coated with an anti-SAA monoclonal antibody and anti-CRP monoclonal antibody to form detection lines T1, T2, T3 and T4. The inflammatory marker POCT joint detection SAA and CRP kit for early and discriminative diagnosis is portable, small in size, simple in operation and convenient in use.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography detection, and in particular relates to a portable household inflammatory marker POCT combined detection kit set. Background technique [0002] Infectious diseases refer to diseases in which various pathogenic microorganisms invade the human body, leading to inflammation or organ dysfunction, which seriously threaten human health and affect the quality of life. Its early diagnosis and rational use of antibiotics play a very important role in controlling the spread of infection. C-reactive protein (CRP) is an acute phase reaction protein synthesized by the liver, which is at a low level during most viral infections; however, it can increase several times, tens of times or even hundreds of times during bacterial infection or injury, The increase range is positively correlated with the degree of bacterial infection. After reasonable treatment, it can be reduced to the normal level in ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577G01N33/533G01N33/68
CPCG01N33/533G01N33/558G01N33/577G01N33/6893G01N2333/47G01N2333/4737
Inventor 王云龙贺沁李玉林王继创程蕾
Owner 河南省生物工程技术研究中心
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