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Test strip for detecting benzodiazepine and preparation method and application method thereof

A benzodiazepine and test strip technology, which is applied in the field of benzodiazepine detection, can solve the problems of inability to realize rapid detection of large-scale samples, complicated operation, long time-consuming and the like, and achieves short detection time, simple operation, Save simple effects

Inactive Publication Date: 2018-12-07
郑州左安检测科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing methods for detecting benzodiazepines, which are complex in operation, time-consuming, and unable to realize the rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, and detection Test strips for detecting benzodiazepines with high speed and low cost, preparation method and application method thereof, so as to realize rapid detection and on-site monitoring of large quantities of benzodiazepine samples

Method used

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  • Test strip for detecting benzodiazepine and preparation method and application method thereof
  • Test strip for detecting benzodiazepine and preparation method and application method thereof
  • Test strip for detecting benzodiazepine and preparation method and application method thereof

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preparation example Construction

[0041] The preparation method of the test strip for detecting benzodiazepine mainly comprises the following steps:

[0042] 1) Label the benzodiazepine antibody with fluorescent microspheres, and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with fluorescent microsphere-labeled benzodiazepine antibody;

[0043] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the hapten-carrier coated with benzodiazepine The detection line of protein conjugates and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of fluoresce...

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Abstract

The invention discloses a test strip for detecting benzodiazepine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate releasepad, a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, benzodiazepine can be fast analyzed. The invention also provides a method for detecting benzodiazepine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of benzodiazepine samples.

Description

technical field [0001] The invention relates to the detection of benzodiazepines, in particular to a test strip for quantitative detection of benzodiazepines by using time-resolved fluorescent microsphere immunochromatography technology, a preparation method and an application method thereof. Background technique [0002] Benzodiazepines are a class of drugs widely used clinically, most of which are derivatives of 1,4-benzodiazepines, mainly including diazepam (stable), fluazepam (flurazepam), chlordiazepoxide, oxime Shazepam and triazolam have sedative-hypnotic, anti-anxiety, anti-convulsant, and muscle-relaxing effects. However, large doses of benzodiazepines can lead to ataxia, acute intoxication can lead to coma and respiratory depression, long-term use can cause physical and mental dependence, and the risk of dependence increases with the increase in dose and the duration of treatment. extended and increased. Once physical dependence occurs, immediate discontinuation ...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/58G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/582G01N33/585G01N33/948
Inventor 贾嘉张振兴吕小翠赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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