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AKTA auto-injection chromatography system and application thereof

An automatic sample injection and chromatography system technology, applied in the direction of analytical materials, instruments, etc., can solve the problems of no AKTA chromatography equipment combined use record, unsolved communication and cooperation problems, etc., to reduce manual intervention, reduce the risk of mistakes, and improve The effect of work efficiency

Inactive Publication Date: 2018-12-07
SHANGHAI WUXI BIOLOGIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this instrument is usually used in conjunction with some spectroscopic analysis instruments. The instrument manufacturer has no record of its use with AKTA chromatography equipment. There has been no solution to the communication and cooperation problem between the two instruments before.

Method used

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  • AKTA auto-injection chromatography system and application thereof
  • AKTA auto-injection chromatography system and application thereof
  • AKTA auto-injection chromatography system and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The configuration and purification of embodiment 1AKTA automatic sampling chromatography system

[0020] The configuration and purification method of the AKTA automatic sampling chromatography system of the present embodiment are:

[0021] Step 1: Configure the I / O Box E9 (GE-29011361) of the AKTA system for connecting external devices, and the cable for connecting the autosampler to the I / O Box E9.

[0022] Step 2: Install the autosampler and connect it to the computer connected to the AKTA Pure device via its own interface.

[0023] Step 3: Install the I / O Box E9, V9(H)-V to the AKTA Pure device.

[0024] Step 4: Connect the syringe needle of the autosampler to the AKTA IS valve, the AKTA IS valve to the V9(H) valve, the V9(H) valve to the sample pump inlet, and the AKTA Outlet valve to the V9(H) valve. Other lines are connected by default. The purpose of this connection method is: use the autosampler to control the injection needle to switch between different samp...

Embodiment 2

[0030] Example 2 Reliability and Stability Verification

[0031] The reliability and stability of the AKTA automatic sampling chromatography system of Example 1 of the present invention are verified by purifying 18 antibody samples with a volume of 20 mL. The specific purification steps are:

[0032] (1) The antibody sample numbers are 1222_1, 1222_2, 1222_3, 1223_1, 1223_2, 1223_3, 1225_1, 1225_2, 1225_3, 1229_1, 1229_2, 1229_3, 1231_1, 1231_2, 1231_3, 1219_1, 1219_2, 1219_1, 1219_2, and 1219_1. These samples were expressed in CHO cells, the fermentation broth was taken, and the supernatant was taken after centrifugal filtration. The 18 20ml supernatants were put into 50ml centrifuge tubes respectively, and then the 18 50ml centrifuge tubes were placed in sequence on the tube rack of the autosampler at one time.

[0033] (2) Disinfect the AKTA system according to the conventional method, and then clean all the pipes to be used with pure water and the corresponding buffer. ...

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Abstract

The present invention discloses an AKTA auto-injection chromatography system. The system comprises an AKTA chromatography device and an autosampler which are interconnected. The high-throughput and fully-automatic purification of proteins is realized by connecting the high-performance autosampler with the AKTA chromatography device commonly used in protein purification, so the working efficiency,the instrument utilization rate and the platform screening / producing capability are greatly improved, manual intervention is reduced, manpower is saved, and the risk of potential errors caused by manual operations is reduced.

Description

Technical field [0001] The present invention relates to the biological field, in particular to high-throughput purification of protein biological macromolecules, and more specifically to an AKTA automatic sampling chromatography system and its application in high-throughput purification of small-volume proteins. . Background technique [0002] Nowadays, monoclonal antibodies or other types of protein biomacromolecule drugs have become very important biomolecules for clinical treatment or research on pathogenic mechanisms. However, for the development of a protein drug or the study of molecular functions, it is often necessary to purify and prepare hundreds of candidate molecules at milligram levels to screen out suitable target molecules. Therefore, purification throughput is the key to rapid screening of suitable target molecules. A key factor is the bottleneck for the rapid preparation of various types of proteins and their numerous derivatives. [0003] The equipment cu...

Claims

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Application Information

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IPC IPC(8): G01N35/10
CPCG01N35/10
Inventor 赵正阳杨剑冰冯竹梅
Owner SHANGHAI WUXI BIOLOGIC TECH CO LTD
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