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Preparation method of bionano material for passivation solidification repairing soil hexavalent chromium pollution

A technology for biological nanomaterials and soil remediation, which is applied in the field of biological material preparation, can solve the problem that the treatment effect needs to be improved, and achieve the effects of strong reduction effect, high efficiency and short pollution time.

Active Publication Date: 2022-03-22
李昕 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial remediation soil Cr 6+ Pollution treatment effect needs to be improved

Method used

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  • Preparation method of bionano material for passivation solidification repairing soil hexavalent chromium pollution
  • Preparation method of bionano material for passivation solidification repairing soil hexavalent chromium pollution
  • Preparation method of bionano material for passivation solidification repairing soil hexavalent chromium pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method for passivating and solidifying soil hexavalent chromium-polluted biological nanomaterials, the steps of which are:

[0043]A, the preparation of composite functional bacteria: Desulfovibrio desulfuricans Sp., Desulforibrio Sp., Desulfotomaculum Sp., Desulfobacterium Sp. Desulfococcus Sp.) according to the mass ratio of 7:50:13:45:8, prepared into composite functional bacteria;

[0044] B. Cultivation of composite functional bacteria: the composite functional bacteria prepared in step A are anaerobically cultured in an anaerobic incubator with sulfuric acid reducing bacteria culture to obtain a culture; the specific culture parameters are: temperature 33°C, pH7 .3, the culture time is 36 hours; the sulphate-reducing bacteria culture is added with 3% sodium lactate and 2% sodium thioglycolate.

[0045] C. Post-processing: The culture obtained in step B is crushed, washed, centrifuged, dried, sealed and packaged under anaerobic conditions.

[0046] ...

Embodiment 2

[0053] A preparation method for passivating and solidifying soil hexavalent chromium-polluted biological nanomaterials, the steps of which are:

[0054] A, the preparation of composite functional bacteria: Desulfovibrio desulfuricans Sp., Desulforibrio Sp., Desulfotomaculum Sp., Desulfobacterium Sp. Desulfococcus Sp.) according to the mass ratio of 7:50:13:45:8, prepared into composite functional bacteria;

[0055] B. Cultivation of composite functional bacteria: the composite functional bacteria prepared in step A are anaerobically cultured in an anaerobic incubator with sulfuric acid reducing bacteria culture to obtain a culture; the specific culture parameters are: temperature 30°C, pH7 .5, the culture time is 48 hours; the sulphate-reducing bacteria culture is added with 1% sodium lactate and 5% sodium thioglycolate.

[0056] C. Post-processing: The culture obtained in step B is crushed, washed, centrifuged, dried, sealed and packaged under anaerobic conditions.

[0057]...

Embodiment 3

[0060] A preparation method for passivating and solidifying soil hexavalent chromium-polluted biological nanomaterials, the steps of which are:

[0061] A, the preparation of composite functional bacteria: Desulfovibrio desulfuricans Sp., Desulforibrio Sp., Desulfotomaculum Sp., Desulfobacterium Sp. Desulfococcus Sp.) according to the mass ratio of 5:35:10:30:7, prepared into composite functional bacteria;

[0062] B. Cultivation of composite functional bacteria: the composite functional bacteria prepared in step A are anaerobically cultured with sulfuric acid reducing bacteria culture in an anaerobic incubator to obtain a culture; the specific culture parameters are: temperature 32°C, pH7 .0, and the culture time was 30 hours; the sodium lactate with a mass content of 5% and the sodium thioglycolate with a mass content of 1% were added to the culture of sulfate-reducing bacteria.

[0063] C. Post-processing: The culture obtained in step B is crushed, washed, centrifuged, dri...

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Abstract

The invention discloses a preparation method of biological nanomaterials for passivation, solidification and restoration of soil hexavalent chromium pollution. , Desulfobacillus and Desulfococcus are formulated into compound functional bacteria according to the mass ratio of 5~10:35~55:10~15:30~60:7~10; B, the cultivation of compound functional bacteria: the compound compound prepared in step A The functional bacteria are cultured anaerobically with sulfate-reducing bacteria in an anaerobic incubator to obtain a culture; the specific culture parameters are: temperature 30-44°C, pH 6.8-8.0, culture time 20-40 hours; C. Post-processing: The culture obtained in step B is crushed, washed, centrifuged, dried, sealed and packaged under anaerobic conditions. The bionano material prepared by the method can passivate and solidify the soil hexavalent chromium pollution, and has a high passivation and solidification rate for the hexavalent chromium in the soil, and the repair effect is good.

Description

technical field [0001] The invention relates to a preparation method of a biological material for repairing soil hexavalent chromium pollutants. Background technique [0002] Chromium produced in chromium salt production, electroplating, tanning, and metal processing, if poorly controlled, can enter the soil directly or through the atmosphere and water, causing soil Cr 6+ (hexavalent chromium) pollution. Cr 6+ It has high activity and high toxicity, and is one of the 47 most dangerous wastes recognized internationally. Remediation of hexavalent chromium pollution in soil by passivation and solidification is to convert Cr in soil 6+ Partially or fully converted to Cr 3+， , thereby reducing its toxicity, reducing or avoiding Cr 6+ Toxic to animals and plants. At present, the passivation and solidification remediation technologies for soil hexavalent chromium pollution mainly include physical remediation, chemical remediation and microbial remediation. [0003] Microbial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCC12N1/20B09C1/10
Inventor 李昕陈春坛张洪荣李卫李福德
Owner 李昕