Radiation pneumonitis CT (Computed Tomography) quantitative detection method used for radiobiology experiment

Abstract

The invention discloses a radiation pneumonitis CT (Computed Tomography) quantitative detection method used for a radiobiology experiment. Through a CT low-dosage scanning parameter, a scanning imageis subjected to three-dimensional reconstruction, then, a CT three-dimensional segmentation image pixel histogram is obtained on the basis of an automatic region growth lung image matrix three-dimensional segmentation technology, a pneumonitis index algorithm is adopted through a pneumonitis degree quantitative criterion, and a mouse pneumonitis index is obtained to finish noninvasive quantitativedetection. The method effectively appears in noninvasive quantitative detection methods which aim at the mouse radiation pneumonitis model at home and abroad for the first time, and is an experimentdetection method which can objectively and accurately quantify the generation and the development of the mouse pneumonitis in real time, and the accuracy, the simplification, the stability and the efficiency of radiation inducibility mouse pneumonitis detection can be obviously improved. While experiment cost and vigor can be greatly reduced, the dependency of small animal minitype CT is reduced,experience cost is lowered, and the method has the obvious advantages of no trauma, high speed in scanning, high accuracy, good repeatability and the like.

Description

technical field [0001] The technology of the present invention belongs to experimental methods, relates to a CT quantitative detection method, in particular to a CT quantitative detection method for radiobiology (mice) experiments of radiation pneumonitis. Background technique [0002] The mouse model of radiation pneumonitis is an important tool for studying the mechanism of normal tissue radiation response, respiratory disease and immune response, and plays an irreplaceable role in the research and development of radiation biology, radiotherapy and anti-lung injury drugs. In the early stage of radiation lung injury, the main manifestation is radiation pneumonitis (usually 1-3 months after secondary radiation), due to the activation of a large number of T lymphocytes to produce an immune response, a type of lymphocytic alveolitis; in the late stage, it can develop into interstitial lung fibrosis (about 6 months after irradiation). Once pulmonary fibrosis occurs, the conseq...

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Problems solved by technology

However, there are still many technical deficiencies in these detection and evaluation methods.

[0004] First, physiological parameters (i.e., respiratory rate, lung compliance, lethality, or body weight) can more objectively reflect abnormal gas exchange due to pulmonary restrictive ventilation, diffuse interstitial inflammation, or scarring, but these parameters are susceptible to many other factors. Interference of factors, such as temperature, infection, combined cardiotoxicity, etc.

[0005] Second, when using low-dose irradiation, only a small amount of pneumonia can be observed in lung tissue sections. These physiological indicators cannot truly reflect lung lesions in the milder lesions or in the self-healing stage: no obvious changes in respiratory rate, Individual death or weight loss (such as X-ray dose <10.5Gy), prone to false negatives

However, in this false negative situation, in mathematical modeling, the measured value of gradient dose (from low to high) is usually required for calculation and simulation, that is, the sensitivity of the detection value of the low dose group will directly affect the accuracy and reliability of the model

[0006] Third, due to the greater compensatory capacity of lung function, for the evaluation of early lung injury (ie, the inflammatory stage), histological evidence of lung interstitial thickening, inflammatory cell proliferation or infiltration is associated with individual death or increased respiratory rate in mice. There is no direct correlation, which affects the accuracy of the results to a certain extent

However, due to the small size of individual mice (the transverse diameter of the lungs of adult mice is only about 1-1.5 cm) and the resolution of CT scanning is limited, even high-resolution or professional micro-CT images cannot accurately diagnose radiation pneumonitis in mice

In view of the above-mentioned defects in the current experiments on radiation-induced lung injury mice, which cannot achieve the purpose of objective and accurate quantitative evaluation, the detection method of the present invention adopts computer-assisted imaging diagnosis (computer-assisted radiology) technology to realize a simple, accurate and quantitative evaluation of pneumonia. developmental approach

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