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Preparation method of composite preparation for preventing and treating soil-borne diseases

A technology of composite preparation and microbial preparation, applied in the field of agricultural microorganisms, can solve the problems of destroying the soil micro-ecological environment, highly toxic to humans and animals, and high cost, and achieves the effects of wide control range, wide parasitic range, and reduced control cost.

Inactive Publication Date: 2018-12-14
杭州渔森农业技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical agents kill insects quickly and have good effects, they are used in a large amount, cost high, are highly toxic to humans and animals, and damage the soil micro-ecological environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a compound preparation for soil-borne diseases, the compound preparation includes Trichoderma viride, Candida and Bacillus cereus.

[0023] The viride is (Trichoderma viride) ATCC No.9645;

[0024] The candida is ( Candida utilis )ATCC No.22023;

[0025] The Bacillus cereus is (Bacillus cereus) ATCC No.10702.

[0026] Preferably, said Trichoderma viride, Candida, Bacillus cereus are composed according to the volume ratio of 3:2:4;

[0027] The preparation method of the composite preparation is as follows: Trichoderma viride, Candida, and Bacillus cereus are respectively cultivated to a concentration of 1×10 7 cells / ml of the bacterial solution, centrifuged to take the supernatant, and then mixed according to the volume ratio of 3:2:4 to obtain the fermentation supernatant mixture, and then mixed with the auxiliary agent according to the volume ratio of 1:5 to prepare;

[0028] The auxiliary agent is: propylene glycol: fatty alcohol polyoxyethy...

Embodiment 2

[0030] A method for preparing a compound preparation for soil-borne diseases, the compound preparation includes Trichoderma viride, Candida and Bacillus cereus.

[0031] The viride is (Trichoderma viride) ATCC No.9645;

[0032] The candida is ( Candida utilis )ATCC No.22023;

[0033] The Bacillus cereus is (Bacillus cereus) ATCC No.10702.

[0034] Preferably, said Trichoderma viride, Candida, Bacillus cereus are composed according to the volume ratio of 4:4:5;

[0035] The preparation method of the composite preparation is as follows: Trichoderma viride, Candida, and Bacillus cereus are respectively cultivated to a concentration of 1×10 7 cells / ml bacterial solution, centrifuged to take the supernatant, and then mixed according to the volume ratio of 4:4:5 to obtain the fermentation supernatant mixture, and then mixed with the auxiliary agent according to the volume ratio of 1:5 to prepare;

[0036] The auxiliary agent is: propylene glycol: fatty alcohol polyoxyethylene et...

Embodiment 3

[0037] Embodiment 3: Toxicological safety test of preparation pesticide of the present invention

[0038]Acute respiratory toxicity test: According to the method in the microbial pesticide toxicology test standard NY / T 2186.1-2012, the mice were randomly divided into four groups, 10 in each group, male and female. The test substance was administered once by intranasal instillation. After nasal instillation, keep the nose of the mouse upward for 30-60 seconds to ensure that the drug solution will not flow out. The treatment group was given the fermentation supernatant mixture prepared in Example 1 and the fermentation supernatant mixture prepared in Example 2 respectively, and the control group 1-2 was given the same volume of uninoculated culture medium and normal saline respectively. Continue to observe for three weeks after the administration, observe the clinical manifestations and death conditions of the mice, and determine the body weight and organ coefficient of the mice...

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Abstract

The invention relates to a preparation method of a composite preparation for preventing and treating soil-borne diseases. The preparation method comprises: mixing Trichoderma viride, Candida, Bacilluscereus and an aid according to a certain ratio. The composite preparation can prevent soil-borne diseases such as root rot, blight and root knot nematode damaging to roots. The composite preparationcreates a suitable soil environment for the root system in advance, promotes the root system to quickly adapt to the soil and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a method for preparing a compound preparation for preventing and controlling soil-borne diseases. Background technique [0002] There are a huge number and variety of microorganisms in the soil, most of which are beneficial, and play an irreplaceable role in soil development, material transformation, structure formation, improving crop nutrient availability, and inhibiting the activity of pathogenic bacteria. However, another type of harmful microorganisms that cause crop diseases also exists in the soil, commonly known as soil-borne pathogenic microorganisms, which lead to the generation of soil-borne diseases. An excellent soil environment breeds a large number and rich variety of beneficial microorganisms. On the contrary, the living environment of harmful microorganisms is relatively harsh, which comes not only from the restrictions of the soil environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/02A01P3/00A01P5/00C12N1/14C12N1/16C12N1/20C12R1/885C12R1/72C12R1/085
CPCA01N63/10A01N63/30C12N1/14C12N1/16C12N1/20
Inventor 曹春花陈爱凤陈立董更强凌云峰
Owner 杭州渔森农业技术开发有限公司
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