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Preparation method for antibacterial peptide from wood frog

A technology of antimicrobial peptide and Rana oil, which is applied in the field of biological extraction to achieve good antibacterial effect

Inactive Publication Date: 2018-12-14
通化县金汇生态农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] my country has a long history of the application of wood frog drugs, but the research on its active ingredients and pharmacological properties mainly focuses on small organic molecules such as alkaloids, and there are few researches on its active peptides

Method used

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  • Preparation method for antibacterial peptide from wood frog
  • Preparation method for antibacterial peptide from wood frog
  • Preparation method for antibacterial peptide from wood frog

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Enzymolysis: Rana oil is used as the main raw material, and the compound enzymolysis method is used for controllable enzymolysis. The obtained enzymolysis solution is light yellow in color, light in fishy smell, slightly sour and bitter, and the degree of hydrolysis is 40%. , the molecular weight of the polypeptide in the hydrolyzed part is mainly distributed between 1000 and 7000 Daltons;

[0032] (2) Deployment: Weigh 25% maltodextrin, 5% β-cyclodextrin, 5% whey protein isolate WPI95, 20% whey protein concentrate WPC80 respectively, put them in a clean and dry container, and stir them thoroughly Slowly added to the enzymolysis solution of a certain quality prepared in step (1) under certain conditions, and magnetically stirred until completely dissolved;

[0033] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat it in a water bath to the emulsification temperature of ...

Embodiment 2

[0037] (1) Enzymolysis: Rana oil is used as the main raw material, and the compound enzymolysis method is used for controllable enzymolysis. The obtained enzymolysis solution is light yellow in color, light in fishy smell, slightly sour and bitter, and the degree of hydrolysis is 40%. , the molecular weight of the polypeptide in the hydrolyzed part is mainly distributed between 1000 and 7000 Daltons;

[0038] (2) Deployment: Weigh 5% maltodextrin, 10% β-cyclodextrin, 20% whey protein isolate WPI95, 5% whey protein concentrate WPC80 respectively, put them in a clean and dry container, and stir them thoroughly Slowly added to the enzymolysis solution of a certain quality prepared in step (1) under certain conditions, and magnetically stirred until completely dissolved;

[0039] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat it in a water bath to the emulsification temperature of ...

Embodiment 3

[0043] (1) Enzymolysis: Rana oil is used as the main raw material, and the compound enzymolysis method is used for controllable enzymolysis. The obtained enzymolysis solution is light yellow in color, light in fishy smell, slightly sour and bitter, and the degree of hydrolysis is 40%. , the molecular weight of the polypeptide in the hydrolyzed part is mainly distributed between 1000 and 7000 Daltons;

[0044] (2) Deployment: Weigh 20% maltodextrin, 1% β-cyclodextrin, 15% whey protein isolate WPI95, 15% whey protein concentrate WPC80 respectively, put them in a clean and dry container, and stir them thoroughly Slowly added to the enzymolysis solution of a certain quality prepared in step (1) under certain conditions, and magnetically stirred until completely dissolved;

[0045] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat it in a water bath to the emulsification temperature of...

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Abstract

The invention discloses a preparation method for an antibacterial peptide from a wood frog. The preparation method comprises the following steps: (1) enzymatic hydrolysis; (2) blending; (3) preparation of an emulsifier; (4) mixing and high-speed shearing; and (5) freeze drying. Wood frog antibacterial peptide powder prepared by using the method has low hygroscopicity, good reconstituability and good bacteriostatic effect; and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and more specifically relates to a preparation method of Rana antibacterial peptide. Background technique [0002] The skin and viscera of amphibians have a wide range of pharmacological activities and clinical curative effects. Many amphibians are widely used in traditional Chinese medicine and national medicines in China, such as the Northeast Rana (Has frog), Chinese toad, and the big webbed bell toad, etc. . Pharmacological activities have been reported to include broad-spectrum antimicrobial effects, antitumor effects, local anesthesia, analgesia, immune regulation, and cardiovascular system effects. [0003] Rana chensinensis, also known as Hash toad, snow clam, etc., belongs to Amphibians, Anura, Ranaidae, and Rana. It is a unique species in my country. The main production areas are distributed in Northeast China, especially in Jilin. In the 1930s and 1940s, Japanese schola...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
CPCC07K1/14C12P21/06
Inventor 龙弘扬龙宏志孔庆军郉云峰
Owner 通化县金汇生态农业发展有限公司