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Grain length gene marker based on genetic background of wheat variety Zhongmai 895 and its application

A technology of wheat and grain length, which is applied in the direction of DNA/RNA fragments, microbial measurement/inspection, recombinant DNA technology, etc. It can solve the problems of time-consuming operation, ineffective molecular assisted breeding, and low throughput.

Active Publication Date: 2018-12-14
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the differences in genetic materials and molecular markers used in research, some QTL intervals are relatively large, and their linkage markers are far from the target functional gene, which cannot be effectively used in molecular assisted breeding
In addition, when molecular markers such as SSR and RFLP used in previous studies are used for genotype identification, the operation is time-consuming and the throughput is low, which is difficult to meet the needs of current and future molecular design breeding

Method used

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  • Grain length gene marker based on genetic background of wheat variety Zhongmai 895 and its application
  • Grain length gene marker based on genetic background of wheat variety Zhongmai 895 and its application
  • Grain length gene marker based on genetic background of wheat variety Zhongmai 895 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1. Acquisition of Interval SNP Marker AX-111538812 and KASP Marker Primer Set for Wheat Grain Length Major QTLQKl.caas-2DL

[0068] Test materials: Zhongmai 895 and Yangmai 16 are one of the main cultivars in the Huanghuai wheat region and the winter wheat region in the middle and lower reaches of the Yangtze River, respectively, with excellent comprehensive agronomic characteristics. One of the main features of Zhongmai 895 is its large grain size and high yield. The present invention uses Yangmai 16 as the female parent and Zhongmai 895 as the male parent to construct a DH population, including 174 families.

[0069] Field experiment: DH populations were planted in Xinxiang, Henan and Luohe experimental stations from 2017 to 2018. Random block design was adopted, and three repetitions were set up. The planting method was planted in plots. Each plot had six rows, the row length was 3m, and the row spacing was 25cm. Field management refers to the management of ...

Embodiment 2

[0080] Example 2. Establishment of a method for detecting the genotype of the SNP marker AX-111538812 using KASP markers

[0081] Detecting Yangmai 16 / Zhongmai 895DH Population Parents and Partial Families Using KASP Marker AX-111538812-KASP Primer Set

[0082] 1. Use the CTAB method to extract the genomic DNA of each family of the Yangmai 16 / Zhongmai 895DH population, and dilute to obtain a template solution with a DNA concentration of about 30 ng / μL.

[0083] 2. Using the genomic DNA extracted in step 1 as a template, PCR amplification was performed using the KASP-labeled primer set described in Example 1 for detecting the SNP marker AX-111538812 in wheat to obtain an amplified product.

[0084] Preparation of KASP labeled primer working solution:

[0085] Take 12 μL (100 μM) of the upstream primer (primer A and primer B) and 30 μL (100 μM) of the downstream primer (primer C) respectively, add to 100 μL with sterile ultrapure water, mix well, and use it as the KASP-labeled ...

Embodiment 3

[0097] Example 3. Application of KASP Marker AX-111538812-KASP in Assisted Identification of Wheat Grain Length in Breeding

[0098] 1. Test materials

[0099] The test materials include: the test group includes 196 wheat varieties (Table 1), and sterile ultrapure water is set as a blank control group.

[0100] Grain length measurement of test group materials: 196 wheat varieties were planted in Henan Luohe Experimental Station in 2017-2018, using a completely randomized block design, with three repetitions, double-row blocks, row length 1.0m, row width 20cm, field management according to Local test station management conducted. After the seeds were ripe and harvested, the method described in Example 1 was used to analyze the length of the repeated grains of the test group, and the average value of the three repetitions was used for statistical analysis.

[0101] 2. Use the KASP marker AX-111538812-KASP primer group to detect the test group and the control group:

[0102] A...

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Abstract

The invention discloses a grain length gene marker based on the genetic background of the wheat variety Zhongmai 895 and its application. The invention provides any of the following applications of asubstance used for detecting whether a deoxyribonucleotide at the site 36 a gene segment shown in SEQ ID No. 4 and located on the chromosome 2D in a wheat genome is A, or G, or A and G: identificationor assisted identification of wheat grain length characters; comparison of the lengths of wheat grains to be tested; and breeding of a single wheat plant or wheat strain or wheat line or wheat variety with relatively long grain length. According to the invention, the 660K SNP high-density genetic map of Yangmai 16 / Zhongmai 895DH populations is utilized for QTL mapping of grain length, and a majorQTL is located on the long arm of the chromosome 2D; and based on the research, a KASP marker special primer set of the flanking marker AX-111538812 is developed, so a good tool is provided for utilization of the grain length QTL QKl. caas-2DL during breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the grain length gene marker and its application based on the genetic background of the wheat variety Zhongmai 895. Background technique [0002] Wheat is an important food crop, which plays a pivotal role in the agricultural industry and food security in my country and the world. Continuously increasing the yield per unit area is the eternal theme of wheat breeding and the basis for meeting the growing human needs. Increasing grain weight is the key to achieving high yield. The essential. Traits such as grain length and grain width are key indicators of grain characteristics, and also affect the processing quality of wheat, and are the key to determining market grading and trade value. [0003] In the past few decades, my country's wheat yield has been greatly improved, the fundamental reason is the introduction of excellent germplasm and the utilization of traditional breeding meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 肖永贵付路平徐小婷杨梦娇扎西次仁夏先春何中虎
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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