Preparation method of SERS substrate of Au@Ag nanoparticles and method for detecting glucose with substrate

A nanoparticle, glucose technology, applied in nanotechnology, nanotechnology, nanotechnology for sensing, etc., can solve the problems of small concentration range of glucose, complicated method, weak Raman signal of cyano group, etc.

Active Publication Date: 2018-12-14
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above method improves the sensitivity of glucose detection to a certain extent, the detection of glucose using this substrate is complicated and requires the addition of glucose molecular probes twice.
And

Method used

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  • Preparation method of SERS substrate of Au@Ag nanoparticles and method for detecting glucose with substrate
  • Preparation method of SERS substrate of Au@Ag nanoparticles and method for detecting glucose with substrate
  • Preparation method of SERS substrate of Au@Ag nanoparticles and method for detecting glucose with substrate

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Experimental program
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Embodiment 1

[0031] A method for detecting glucose in urine, comprising the following steps:

[0032] 1) Add 90mL distilled water to 10mL, 2.4mmol / L chloroauric acid solution, heat to boiling, add 1% sodium citrate solution and react for 15min to obtain gold nanoparticles.

[0033] 2) The gold nanoparticles were reacted with 4-mercaptoaniline solutions of different concentrations (0.05mmol / L, 20μL) for at least 12 hours, and then washed by centrifugation.

[0034] 3) Add 100mmol / L silver nitrate solution, 100mmol / L ascorbic acid and 100mmol / L sodium hydroxide solution in sequence by volume ratio (0.6:25:50) to 20mL of gold nanoparticles prepared in step (2), and react at room temperature for 4 hours , to restore the nano-silver shell to obtain the Au@Ag nano-core-shell structure particles we need, centrifuge and wash, and take the lower layer solution to become the Au@Ag nano-core-shell structure colloid.

[0035] 4) Take 20mL of the prepared Au@Ag nano core-shell structure colloid, add (...

Embodiment 2

[0039] 1) Add 90mL distilled water to 10mL, 2.4mmol / L chloroauric acid solution, heat to boiling, add 1% sodium citrate solution and react for 15min to obtain gold nanoparticles.

[0040] 2) The gold nanoparticles were reacted with 4-mercaptoaniline solutions of different concentrations (0.5mmol / L, 30μL) for at least 12 hours, and then washed by centrifugation.

[0041] 3) Add silver nitrate solution, ascorbic acid, and sodium hydroxide solution to 2) in proportion (0.6:25:50) in turn, react at room temperature for 4 hours, and restore the nano-silver shell to obtain the Au@Ag nano-core we need The particles with the shell structure are washed by centrifugation, and the solution of the lower layer is Au@Ag nano core-shell structure colloid.

[0042] 4) Take the prepared Au@Ag nano core-shell structure colloid, add (1mmol / L, 10μL) 4-mercaptophenylboronic acid solution, react for 2 hours, control the temperature at about 60°C, and centrifuge and wash.

[0043] 5) React the gluc...

Embodiment 3

[0046] 1) Add 90mL distilled water to 10mL, 2.4mmol / L chloroauric acid solution, heat to boiling, add 1% sodium citrate solution and react for 15min to obtain gold nanoparticles.

[0047] 2) The gold nanoparticles were reacted with 4-mercaptoaniline solutions of different concentrations (5 mmol / L, 30 μL) for at least 12 hours, and then washed by centrifugation.

[0048] 3) Add silver nitrate solution, ascorbic acid, and sodium hydroxide solution to 2) in proportion (0.6:25:50) in turn, react at room temperature for 4 hours, and restore the nano-silver shell to obtain the Au@Ag nano-core we need The particles with the shell structure are washed by centrifugation, and the solution of the lower layer is Au@Ag nano core-shell structure colloid.

[0049] 4) Take the prepared Au@Ag nano core-shell structure colloid, add (1mmol / L, 20μL) 4-mercaptophenylboronic acid solution, react for 2 hours, control the temperature at about 60°C, and wash by centrifugation.

[0050] 5) React the g...

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Abstract

The invention discloses a preparation method of an SERS substrate of Au@Ag nanoparticles. According to the method, gold nanoparticles are prepared by reducing chloroauric acid with sodium citrate, thegold nanoparticles are modified with 4-sulfydryl aniline to link silver ions, and a certain proportion of ascorbic acid and sodium hydroxide are added to reduce a silver shell. Meanwhile, the form size of the core-shell structure is controllable, plasma resonance is realized through high coupling of the structure, and the so-called hotspots are formed in the core-shell structure, so that 4-sulfydryl aniline serving as an internal standard molecule has very high Raman signals. The Au@Ag core-shell structure is modified with 4-sulfydryl phenylboronic acid to capture glucose molecules, so that qualitative and quantitative detection of glucose is realized. The method has the advantages as follows: the internal standard substance is used for signal comparison, interference of complex substances in samples (such as urine) can be avoided, the detection sensitivity is improved, the preparation cost is low, and qualitative and quantitative detection of glucose is really realized.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for preparing a SERS substrate of Au@Ag nanoparticles and a method for detecting glucose by using the substrate. Background technique [0002] Diabetes (diabetes) is an endocrine and metabolic disease mainly manifested by glucose metabolism disorders. In recent decades, the number of diabetics worldwide has grown at an astonishing rate. It has become a major public health problem that seriously affects the physical and mental health of Chinese people. There is currently no effective treatment for it, and it can produce many complications. Therefore, early prevention appears to be particularly important. Plasma glucose is currently the only reliable diagnostic index for diabetes, and it is also the main basis for judging the condition and control of diabetes. [0003] Currently commonly used detection methods for blood glucose detection include: fasting pl...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y15/00B82Y30/00B82Y40/00
CPCB82Y15/00B82Y30/00B82Y40/00G01N21/658
Inventor 周婷卢玉栋朱兰瑾吴阳卢仲柱陈玉婷
Owner FUJIAN NORMAL UNIV
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