Application of cowherb seed flavonoid glycoside capable of preventing microcirculation disturbance
A kind of Wangbuliuxing flavonoid glycosides, microcirculation disorder technology, applied in the field of drug development
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Embodiment 1: the preparation of varicose flavonoid glycosides
[0024] Specific steps are as follows:
[0025] (1) Grind blanc blanc into pieces, degrease with petroleum ether and weigh 50g;
[0026] (2) 50 g of the obtained defatted bulipel was extracted with 75% ethanol under reflux for 90 min, and the filtrate was extracted twice and combined twice;
[0027] (3) Redissolve the obtained filtrate with deionized water after spin-drying, then extract with water-saturated n-butanol to obtain a water-saturated n-butanol extraction part;
[0028] (4) Spin-dry the obtained water-saturated n-butanol extraction part and reconstitute it with ionized water to form a 2 mg / mL medicinal solution;
[0029] (5) Soak the HPD-100 macroporous resin in 95% ethanol overnight, stir it evenly with a glass rod and drain it into a glass column with a diameter of 2.5cm and control the ratio of diameter to height to 1:7 (18cm, 2.5cm) ;
[0030] (6) Wash the macroporous resin with 95% ethan...
Embodiment 2
[0033] Embodiment 2: the preparation of mouse model
[0034] Fifty C57BL / 6 mice were randomly divided into 5 groups, namely, blank control group, model group, high-dose agaricus flavonoid glycosides group, low-dose acaricus glucosides group and positive drug group, 10 in each group. Only, wherein, the blank control group was fed with common feed, and the remaining four groups were fed with high-fat feed (the high-fat feed contained 1% lard, 5% egg yolk powder, 1% cholesterol, 10% sugar, 0.2% sodium cholate) for a total of 8 weeks.
Embodiment 3
[0035] Embodiment 3: Administration of mice
[0036] After 8 weeks, the blank control group obtained in Example 2 was injected intraperitoneally with 0.4 mL of normal saline every day, and the flavonoid glucoside high and low dose groups were fed with 0.4 mL of 1 mg / kg and 10 mg / kg respectively. 0.05mg / mL, 0.5mg / mL of the flavonoid glycoside solution of blalicius (formed from the flavonoid glycoside obtained in Example 1 and normal saline), and the positive drug group was intraperitoneally injected with a concentration of 0.4mL at 200mg / kg. 0.025 mg / mL metformin solution (prepared from metformin purchased from Sigma and normal saline).
[0037] The basic conditions of the mice, such as body weight and living conditions, were recorded for a total of 12 weeks of administration.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com