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Microfluidic chip, chip manipulation system and microfluidic apparatus for nucleic acid extraction, and nucleic acid extraction method

A microfluidic chip and manipulation system technology, applied in the fields of diagnosis and detection, and biomedicine, can solve the problems of increasing the error probability of nucleic acid extraction operations and reducing the efficiency of nucleic acid extraction, so as to reduce the probability of operation errors, save operation time, and improve efficiency Effect

Pending Publication Date: 2018-12-18
BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, when microfluidic chips are used for nucleic acid extraction, various reagents for nucleic acid extraction still need to be added to the inside of the microfluidic chip, which reduces the efficiency of nucleic acid extraction and increases the cost of nucleic acid extraction. operation error probability

Method used

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  • Microfluidic chip, chip manipulation system and microfluidic apparatus for nucleic acid extraction, and nucleic acid extraction method
  • Microfluidic chip, chip manipulation system and microfluidic apparatus for nucleic acid extraction, and nucleic acid extraction method
  • Microfluidic chip, chip manipulation system and microfluidic apparatus for nucleic acid extraction, and nucleic acid extraction method

Examples

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no. 1 example

[0104] Figure 1 to Figure 1 2 shows the structure of the microfluidic device for nucleic acid extraction and its microfluidic chip according to the first embodiment of the present invention. The microfluidic device includes a microfluidic chip 27 for nucleic acid extraction and a chip manipulation system for nucleic acid extraction. The chip manipulation system is coupled with the microfluidic chip 27 and controls the microfluidic chip 27 to perform nucleic acid extraction.

[0105] see figure 1 , Figure 7 and Figure 8 . In the first embodiment, the microfluidic chip 27 includes a storage cavity cover 50, a reagent storage structure 54, a puncture structure 55, a communication structure 56, a reaction structure 60, and a waste liquid storage structure arranged in sequence from top to bottom. Sheet 66 and bottom sheet 69 of the waste chamber. In addition, the elution chamber cover sheet 65 is also included.

[0106] The storage chamber cover sheet 50 is located above ...

no. 2 example

[0178]As shown in Figure 13, the difference between the second embodiment and the first embodiment is: in the microfluidic chip 27, the mixing chamber 61 and the elution chamber 63 are vertical structures, and the hose 62 is vertically placed and connected to the mixing chamber 61 Between the elution chamber 63 , the waste liquid removal channel 70 is located at the bottom of the elution chamber 63 , and the waste liquid chamber air hole 68 is located at the top of the waste liquid chamber 67 . Correspondingly, the closing slider 23 of the chip manipulation system is located on the side of the microfluidic chip 27, and driven by the hose closing motor 21, the closing slider 23 moves horizontally to squeeze the hose 62 to achieve the closing effect. At the same time, the heating film 24 fixed on the closing slider 23 is attached to the side wall of the elution chamber 63 .

[0179] When the waste liquid in the mixing chamber 61 needs to be discharged to the waste liquid chamber...

no. 3 example

[0182] Such as Figure 14 As shown, the difference between the third embodiment and the first embodiment is that the pressure control component of the chip handling system is not a vacuum suction component, but a solenoid valve 73 and a rubber plug 72 connected to the end of the solenoid valve 73 . Wherein, the gas hole 68 of the waste liquid chamber is arranged above the waste liquid chamber 67 , and the solenoid valve 73 is used to control the opening and closing of the gas hole 68 of the waste liquid chamber.

[0183] The electromagnetic valve 73 drives the rubber plug 72 to close the waste liquid chamber pore 68 downwards when it is in the open state, so that the inside of the waste liquid chamber 67 forms a closed space. During the reaction process, the liquid reagent in the mixing chamber 61 stays at The mixing chamber 61 does not flow downward. The solenoid valve 73 drives the rubber stopper 72 to move upwards in the closed state, the waste liquid chamber air hole 68 i...

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Abstract

The invention discloses a microfluidic chip, a chip manipulation system and a microfluidic apparatus for nucleic acid extraction, and a nucleic acid extraction method. The microfluidic chip comprisesat least a storage cavity, at least a reagent communication control part and at least a reaction cavity, wherein each storage cavity and one reaction chamber are correspondingly, the reagent communication control part is arranged between each storage cavity and the corresponding reaction cavity so as to control whether the storage cavity and the corresponding reaction cavity are communicated, anda reagent for nucleic acid extraction is encapsulated in at least the one the storage cavity. According to the present invention, the nucleic acid extraction operation steps can be simplified, the automated nucleic acid extraction can be achieved, the operation time for the nucleic acid extraction can be saved, the nucleic acid extraction efficiency can be increased, and the probability of the operation error can be reduced.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, diagnosis and detection, in particular to a microfluidic chip, a chip manipulation system, a microfluidic device and a nucleic acid extraction method. Background technique [0002] The high sensitivity and specificity of nucleic acid detection makes it occupy an extremely important position in the field of biological detection technology. The premise of nucleic acid detection is to obtain highly purified nucleic acid templates from biological samples. The existing nucleic acid extraction process often includes multiple complex biological reaction steps. Whether it is done manually or with auxiliary equipment, it is inefficient and error-prone. Waiting is not enough. [0003] The microfluidic chip can transplant the basic operation units such as sample preparation, mixing, separation, reaction, detection, cell culture, and sorting in the process of chemical, biological, and medical analysis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/00C12N15/10
CPCC12N15/1013
Inventor 邱宪波邱子欣叶祥忠王东冯梁向飞李益民
Owner BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE
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