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Chemiluminiscence immunoassay microfluidic chip, detector and detection method

A chemiluminescence immune, microfluidic chip technology, applied in the field of microfluidics, can solve the problems of complex instruments, increased chip cost, and high processing accuracy requirements, and achieve the effect of simple operation

Pending Publication Date: 2018-12-18
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reagents are not integrated on the chip, which makes the instrument complex and large-scale. The design and processing accuracy of the dam behind the reaction chamber is high, which leads to an increase in the cost of the chip.

Method used

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  • Chemiluminiscence immunoassay microfluidic chip, detector and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] A chemiluminescent immunoassay microfluidic chip, comprising a square substrate, a fixed groove is arranged in the geometric center of the substrate, and the substrate is also provided with a sampling unit, a distribution unit, a reaction unit and a waste liquid recovery unit connected in sequence through microchannels; adding samples The distances from the unit, the distribution unit, the reaction unit and the waste liquid recovery unit to the centrifugal center increase sequentially; a magnet arranged on one side of the substrate is also included to fix the magnetic reactant produced by the reaction unit;

[0098] The sampling unit includes a sampling hole;

[0099] the dispensing unit includes a first mixing tank;

[0100] The reaction unit includes a reaction tank, and a curing agent layer is fixed in the reaction unit for immune reaction with the sample to be tested;

[0101] The waste liquid recovery unit includes a first waste liquid tank;

[0102] The sampling...

Embodiment 2

[0110] A chemiluminescent immunoassay microfluidic chip, comprising a circular substrate, the geometric center of which is provided with a fixed groove, and the substrate is also provided with a sampling unit, a liquid storage unit, a distribution unit, a reaction unit and a waste liquid recovery unit; The distance from the centrifugal center to the unit, distribution unit, reaction unit and waste liquid recovery unit increases in turn;

[0111] The sampling unit includes a sampling hole, a first quantitative tank and a second waste liquid tank; the sampling hole, the first quantitative tank and the second waste liquid tank are sequentially connected through a microchannel;

[0112] The liquid storage unit includes a cleaning liquid storage tank and a substrate liquid storage tank; the cleaning liquid storage tank and the substrate liquid storage tank have the same structure, and both include a liquid storage tank body, and the inside of the liquid storage tank body is provided...

Embodiment 3

[0134] A chemiluminescent immunoassay microfluidic chip, such as figure 1 As shown, it includes a circular substrate, a fixed groove 1 is set in the geometric center of the circular substrate, and the substrate is also provided with a sampling unit, a liquid storage unit, a distribution unit, a reaction unit and a waste liquid recovery unit; the sample addition unit, the distribution unit, the reaction The distance between the unit and the waste liquid recovery unit from the centrifugal center increases sequentially;

[0135] The sampling unit comprises a sampling hole 2, a first quantitative tank 7, a second waste liquid tank 8 and a residue collection tank 10; the sampling hole 2 communicates with the first quantitative tank 7 through the first microchannel 4, and the second waste liquid Liquid tank 8 is communicated with the first quantitative tank 7 by the second microchannel 5, and the residue collection tank 10 is communicated with the first quantitative tank 7 by the th...

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Abstract

The invention provides a chemiluminiscence immunoassay microfluidic chip, a detector and a detection method. The chip comprises a substrate, wherein the substrate is provided with a sample injection unit, a distribution unit, a reaction unit and a waste liquor recovery unit, which are communicated in sequence through a microchannel; the sample injection unit comprises a sample injection hole; thedistribution unit comprises a first mixing groove; the reaction unit comprises a reaction groove; a curing agent layer which is used for generating immunoreaction with a to-be-detected sample is fixedin the reaction unit; and the waste liquor recovery unit comprises a first waste liquor groove. According to the chemiluminiscence immunoassay microfluidic chip, all the reagents can be previously embedded in the chip, so that the advantages that the large-scale instruments are accurate and controllable in detection result and the traditional immediate detection is rapid and simple to operation are both considered; fluid control is realized through centrifugation and sample quantification is realized through a quantification hole; and synchronous detection of multiple targets can be realized.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and relates to a chemiluminescent immunodetection microfluidic chip, a detector and a detection method. Background technique [0002] Due to its high sensitivity and high specificity, chemiluminescent immunoassay has basically replaced radioimmunoassay and enzyme-linked immunoassay, and has become the most widely used immunoassay in the field of immunoassay and diagnosis. [0003] There are two main types of chemiluminescence immunoassay analyzers currently on the market, one is a fully automatic chemiluminescence immunoassay analyzer and the other is a semi-automatic chemiluminescence immunoassay analyzer. [0004] The fully automatic instrument realizes the automatic processing of the entire chemiluminescent immunoassay process, including sample injection, sample addition, reagent addition, incubation, cleaning, magnetic separation, and luminescence detection. The test process does not re...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/543B01L3/00
CPCG01N33/531G01N33/54326B01L3/5027
Inventor 焦政顾志鹏李重阳刘仁源李建霖姜桂华
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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