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Culture method for agaric cultivation stocks

A cultivation method and original seed technology, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of mycelium death, etc., and achieve the effect of accelerating growth rate, improving efficiency and reducing the possibility of bacterial infection.

Inactive Publication Date: 2018-12-21
望谟县郊纳八步农业综合投资开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current method of cultivating the original species of fungus mainly adopts the conventional culture medium after high-temperature and high-pressure sterilization, and the culture time is 30-40 days, and during the culture process, many mycelia may die due to infection of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment provides a kind of preparation method of the original species of fungus cultivation, and the specific process is as follows:

[0025] 1. Activation of parent species: Take 1 part of potassium dihydrogen phosphate, 0.5 parts of magnesium nitrate, 5 parts of peptone, 10 parts of glucose, 20 parts of agar, and 1000 parts of water, mix evenly, heat and melt, cool to room temperature, add 0.5 parts of antibiotics, as Mother culture medium, the mother culture medium is sterilized at a temperature of 120°C and a pressure of 0.2 MPa for 2 hours, cooled to room temperature, and inoculated into the culture medium under aseptic operation to activate the Auricularia auricula species, 0.5 parts of antibiotics, activated Room temperature 23°C, activation time 8d;

[0026] 2. Preparation of the original seed medium: Take 70 parts of poplar wood chips, 10 parts of corn husks, 20 parts of rice flour, add 8 parts of potatoes to make potato soup, mix evenly with water, squ...

Embodiment 2

[0033] This embodiment provides a kind of preparation method of the original species of fungus cultivation, and the specific process is as follows:

[0034] 1. Mother species activation: Take 1.5 parts of potassium dihydrogen phosphate, 0.8 parts of magnesium nitrate, 6 parts of peptone, 13 parts of glucose, 23 parts of agar, and 1000 parts of water, mix evenly, heat and melt, then cool to normal temperature, add 1.2 parts of antibiotics, as Mother culture medium, the mother culture medium is sterilized at a temperature of 125°C and a pressure of 0.2MPa for 3 hours, cooled to room temperature, and inoculated into the culture medium with black fungus strains under aseptic operation for activation, 1 portion of antibiotics, activation Room temperature 24°C, activation time 9d;

[0035] 2. Preparation of the original seed medium: Take 75 parts of poplar wood chips, 13 parts of corn husks, 23 parts of rice flour, add 9 parts of potatoes to make potato soup, add water and mix evenl...

Embodiment 3

[0042] This embodiment provides a kind of preparation method of the original species of fungus cultivation, and the specific process is as follows:

[0043] 1. Activation of mother species: Take 2 parts of potassium dihydrogen phosphate, 1 part of magnesium nitrate, 8 parts of peptone, 15 parts of glucose, 25 parts of agar, and 1000 parts of water, mix evenly, heat and melt, cool to normal temperature, add 2 parts of antibiotics, as Mother culture medium, the mother culture medium is sterilized at a temperature of 130°C and a pressure of 0.2MPa for 4 hours, cooled to room temperature, and inoculated into the culture medium with black fungus strains under aseptic operation for activation, 2 parts of antibiotics, activation Room temperature 25°C, activation time 10d;

[0044]2. Preparation of the original seed medium: Take 80 parts of poplar wood chips, 15 parts of corn husks, 25 parts of rice flour, add 10 parts of potatoes to cook the potato soup, add water and mix evenly, squ...

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Abstract

The invention belongs to the technical field of fungus planting, and particularly relates to a culture method for agaric cultivation stocks. In a stock culture period, hyphae need to secrete all kindsof enzymes to perform enzymolysis on cellulose, lignin and others with existing carbonhydrate, nutrient substances are degraded into carbonhydrate to be adsorbed, through the method that mixed liquorof cellulose, lignin and peroxidase is added into a culture medium for fermentation, a lot of substances beneficial to adsorption of the hyphae are obtained through degrading in the culture medium, in this way, the growing speed of the hyphae is increased, culture time is shortened, the culture medium is also degraded more completely, and the effect of culture is improved; through ultrasonic andhigh-temperature sterilizing treatment and by adding anti-bacterial liquor composed of rose Bengal and streptomycin, bacteria inside the culture medium can be sufficiently killed through the ultrasonic and high-temperature treatment, the rose Bengal and streptomycin are inhibitors for the bacteria and actinomycetes, and will not inhibit fungi of agaric, in this way, the probability that the culture medium is infected with the bacteria is lowered, and the efficiency of the culture medium is improved.

Description

technical field [0001] The invention belongs to the technical field of fungus planting, and in particular relates to a method for cultivating original species of fungus cultivation. Background technique [0002] Fungus, also known as black fungus, light fungus. Black fungus is a saprophytic mesophilic fungus, the hyphae can grow between 6-36°C, but the most suitable temperature is 22-32°C; fruiting bodies can be differentiated at 15-27°C, but the most suitable temperature is 20-24°C , mycelium can grow in the cultivation material and wood with a water content of 60% to 70%, and can grow normally in the dark; when the fruiting body is formed, the water content of the fungus is required to reach more than 70%, and the relative air humidity is 90% to 95%. The fruiting body needs 250-1000Lx light intensity during the growth period. It is an aerobic fungus, and the pH value is 5-5.6. Cultivation methods include section wood cultivation and plastic bag substitute cultivation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 吴应凯
Owner 望谟县郊纳八步农业综合投资开发有限公司