A kind of rapid propagation method of safflower tissue

Abstract

The invention discloses a rapid carthamus tinctorius tissue propagation method which comprises the following steps: by taking carthamus tinctorius aseptic seedling stems as explants, inducing embryogenic calli, carrying out subculture seedling culture, carrying out rooting induction, and carrying out domestication transplanting, thereby obtaining regenerated plants of carthamus tinctorius. By adopting the method, a high embryogenic callus rate and a high callus re-reduced seedling rate can be achieved, a high survival rate can be achieved after tissue culture seedlings are domesticated and transplanted, the method is applicable to industrial production of carthamus tinctorius seedlings, and a base can be made for later transgenosis application.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of safflower tissue. Background technique [0002] Safflower (Carthamus tinctorius L.) is an annual herb of the genus Carthamus in the Compositae family. It is called yellow-blue flower with yellow color. Originally produced in Egypt, it is cultivated in Pakistan, India and other places. It was introduced to my country in the early Han Dynasty, and it was first recorded in "Kaibao Materia Medica" and listed as a middle grade. It has a cultivation history of more than 2,100 years. my country's safflower production areas are mainly concentrated in Xinjiang, followed by Sichuan and Yunnan provinces. The stem of safflower is cylindrical, with thin and shallow grooves, 40-100cm high, lignified at the base, and multi-leaved alternate at the upper part, nearly sessile and slightly clasping, ovate-lanceolate or egg-rowed, slightly thi...

AI Technical Summary

Problems solved by technology

For example, the Indian safflower varieties Mangira and A-1 reported by Tejovathi et al. had very low frequency of regenerated shoots induced from callus, and the reproducibility was poor. In the 1990s, Prasad et al. Research was carried out and it was found that MS medium was easy to induce haploid callus. When 2.0mg / L 6-BA and 0.5mg / L NAA were added, clustered shoots could grow from haploid callus, and the buds were transferred to Roots can grow on 1 / 2MS+0.1mg / L NAA+1% sucrose, so as to grow into normal plants. Ying et al. used Agrobacterium tumefaciens to mediate the formation of the transgenic American safflower variety Centennial, and compared the plant’s Regeneration was studied, shoots were obtained from cotyledon and leaf induced calluses, germination rate was 26%, rooting attempts were unsuccessful

Judging from the current research status, for the culture of safflower callus, reproductive organs such as cotyledons, ovules, and hypocotyls are mostly used as explants for culture, while for other organs, such as stems, the research carried out in the prior art is relatively limited. For example, Ying et al. used Agrobacterium tumefaciens to form the stalks of the transgenic safflower variety Centennial produced in the United States for callus culture. Although a higher callus rate could be obtained, the callus Unsatisfactory germination rate of wounded tissue, and unsuccessful rooting attempts

Zhu Zhenxian used safflower stems as explants, but the healing rate was not ideal

It can be seen that in the prior art, safflower stalks are used as explants for callus culture, but good germination rate and rooting effect have not been obtained, and there are few reports on whether it can be transplanted and survived.

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