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Enrichment purification process for effective part of rhus chinensis

A kind of technology of fenugreek and parts is applied in the field of enrichment and purification process of effective parts of fenugreek phenolic acids, and achieves the effect of simple purification production process and high yield.

Active Publication Date: 2018-12-21
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as Li Jialin, Wu Suzhen, Bei Zhanyu. Research on the extraction process of total flavonoids of Saltwort [J]. But not related to phenolic acid compounds in Salmonella

Method used

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  • Enrichment purification process for effective part of rhus chinensis
  • Enrichment purification process for effective part of rhus chinensis
  • Enrichment purification process for effective part of rhus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 total phenolic acid enrichment and purification process

[0033] (1) Extraction of the total phenolic acids of P. chinensis:

[0034] Take by weighing 300 g of the Alba chinensis coarse powder after crushing and sieving (45 mesh), add 20 times the volume fraction of 70% ethanol solution, soak for 30 min before extraction, then reflux for 90 min, extract 2 times, combine the filtrates twice, and decompress Concentrate until there is no alcohol smell, and then you can get the total phenolic acid solution of S. chinensis.

[0035] (2) Separation and purification of the total phenolic acids of Salmonella:

[0036] a. Macroporous resin pretreatment:

[0037] Fully soak 9g of SP825L macroporous resin with 95% ethanol, and pack it wet after 24 hours. After packing, the ratio of diameter to height of the macroporous resin column is 1:5. First, elute the column bed with 95% ethanol until the effluent is mixed with 5 times the amount of distilled water and no whit...

experiment example 1

[0041] Extraction of Experimental Example 1 Total Phenolic Acids

[0042] Instruments and reagents

[0043] 1 instrument

[0044] Shimadzu UV-1800 visible spectrophotometer (Shimadzu, Japan), CPA225D one-hundred-thousandth balance (product of Sartorius Scientific Instrument Co., Ltd.), SHB-III circulating water multi-purpose vacuum pump (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd. company), LX-07A multifunctional pulverizer (Shanghai Jiangxin Technology Co., Ltd.), HH-Z digital display constant temperature water bath (Changzhou Guohua Electric Appliance Co., Ltd.).

[0045] 2 reagents

[0046] Rhus chinensis (batch number 15122201, Anhui Xiehecheng Pharmaceutical Pieces Co., Ltd.), identified as the dry rhizome of Rhus chinensis Mill. by senior experimenter Fan Shiming, School of Pharmacy, Fujian University of Traditional Chinese Medicine, samples stored in Biomedicine, Fujian University of Traditional Chinese Medicine R & D center; gallic acid reference su...

experiment example 2

[0070] Purification of Experimental Example 2 Total Phenolic Acids of Salmonella

[0071] Instruments and materials

[0072] 1 instrument

[0073] UV9100 UV-Vis spectrophotometer (Beijing Rayleigh Analytical Instrument Co.); CPA225D 1 / 100,000 analytical balance (Germany Sartorius); 1 / 10,000 balance (Sartorius Scientific Instrument Co., Ltd.); KQ-500E Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); FY135 Chinese herbal medicine pulverizer (Tianjin Test Instrument Co., Ltd.); HC-2068 high-speed centrifuge (Anhui Zhongjia Scientific Instrument Co., Ltd.); ZHWY-200D constant temperature culture Oscillator (Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.); RE-2000A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); SHB-III circulating water multi-purpose vacuum pump (Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.); KW-1000DC type Constant temperature water bath HH-1 (Guohua Electric Co., Ltd.); oven.

[0074] 2 reagents ...

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Abstract

The invention relates to an enrichment purification process for the effective part of rhus chinensis, and belongs to the field of purification of effective parts of traditional Chinese medicines. A preparation method of the total phenolic acid part of rhus chinensis comprises the following steps: (1) extraction: extracting a rhus chinensis powder with 50-70% ethanol, filtering, and concentrating the filtrate under reduced pressure till no ethanol smell to obtain a total phenolic acid solution of rhus chinensis; and (2) macroporous resin purification: adsorbing the total phenolic acid solutionof rhus chinensis with macroporous resin, washing with water to remove impurities, and eluting with a 50-90% ethanol solution to obtain a total phenolic acid of rhus chinensis. The method can improvethe total phenolic acid yield of the rhus chinensis, and provides a reasonable, economic and simple purification production process for industrialized production of the total phenolic acid of rhus chinensis.

Description

technical field [0001] The invention belongs to the field of purification of effective parts of traditional Chinese medicines, and in particular relates to a process for enriching and purifying effective parts of lignan acids. Background technique [0002] Rhus chinensis Mill. is a deciduous small tree of the Rhus chinensis Mill. "Kaibao Materia Medica" records that Saltwood has the effects of dispelling wind and dampness, reducing swelling and softening firmness, promoting body fluid and moistening lungs, reducing fire and reducing phlegm, astringent and detoxifying, etc. Saltwood is commonly used by folks to treat symptoms such as lung deficiency cough and chest pain. According to traditional Chinese medicine, Saltwood has the functions of diuretic and swelling, promoting blood circulation and removing blood stasis, dispersing Qi and relieving pain. According to the research of modern pharmacology, it has the effect of improving coronary circulation and blood rheology. ...

Claims

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Application Information

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IPC IPC(8): A61K36/22
CPCA61K36/22A61K2236/33A61K2236/51A61K2236/55
Inventor 徐伟叶淼许文褚克丹林羽
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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