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Microbial agent for decomposing TVOC (total volatile organic compound) and preparation method of microbial agent

A technology of microbial inoculants and strains, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of removing harmful substances, and achieve the effect of improving air quality and protecting health.

Pending Publication Date: 2018-12-25
侯希波 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although these methods can remove TVOC to a certain extent, they cannot really remove harmful substances fundamentally.
At present, the method of using microorganisms to remove TVOC has appeared, but there is no single method of using microbial agents to remove TVOC

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Among them, rice vinegar is a mixture containing acetic acid obtained by using starchy substances through cooking, gelatinization, liquefaction and saccharification. The preparation method at least includes: using amylase to convert starch into sugar, then adding yeast to ferment to produce ethanol, Add acetic acid bacteria to continue to oxidize ethanol to acetic acid, and rice vinegar can be prepared. The starchy substance is selected from at least one of glutinous rice, rice, sorghum, millet, corn, sweet potato and potato.

[0029] Liquor can be sorghum wine, corn wine, glutinous rice wine, sweet potato wine. Above-mentioned alcoholic beverages all take grain as main raw material, the conventional liquor that makes through fermentation. It can be obtained commercially or can be prepared by fermentation.

[0030] In one embodiment of the present invention, the base liquid of the fermentation broth also contains potassium dihydrogen phosphate (KH 2 PO 4 ), magnesiu...

Embodiment 1

[0039] 1) Isolation and screening of pure strains:

[0040] Three methods can be used for separation: Streak separation method: use an inoculation needle to pick a microbial sample and draw a line on the surface of the solid medium, and cultivate under appropriate conditions to obtain a single colony; Separation method by coating: Dip the smear stick into the culture solution , or first drop a small amount of culture solution on the surface of the solid medium, and spread it evenly on the surface of the solid medium with a coating rod; dilution separation method: mix the solid medium down to about 50°C with a certain amount of bacterial suspension Afterwards, cast into plates to obtain single colonies.

[0041] 2) Preparation of fermentation broth base liquid:

[0042] It is obtained by mixing white vinegar and corn wine with a solution of sucrose. In the fermentation broth base liquid, the volume percentage of acetic acid is 0.5%, the volume percentage of ethanol is 1%, and...

Embodiment 2

[0049] On the basis of embodiment 1, increase step 4):

[0050] 4) Add solvent containing plant fragrance

[0051] After the fermentation is stopped, the fermented liquid is mixed with a solvent containing plant flavors. Botanical fragrances include mint, lemon, lavender, rosemary, borneol, wormwood. The above-mentioned plant spices are added together into water at 80° C., and soaked for 10 to 30 minutes to remove solid matter. The solvent in which the plant fragrance is dissolved is mixed with the fermented liquid, and the mass ratio of the two is 1: (5-10) to obtain the microbial bacterial agent.

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PUM

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Abstract

The invention relates to a microbial agent for decomposing TVOC (total volatile organic compound). Strains are added into fermentation liquid base solution and fermented to obtain the microbial agentand include rhodopseudomonas, rhodospirillum, lactobacillus, candida, nitrate bacteria, streptomyces and chromatium, and the total bacteria density of the microbial agent is (1-10)*10<8> bacteria / L. The microbial agent can be directly sprayed into air to treat the TVOC after dilution. Harmful substances in the TVOC can be purified, decomposed and transferred into harmless and odorless substances.Pathogenic bacteria such as pathogenic escherichia coli, mycetes, staphylococcus aureus and candida albicans can be removed, air quality is thoroughly improved, and fitness is protected.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a microbial agent for decomposing TVOC and a preparation method of the microbial agent. Background technique [0002] TVOC is a general term for indoor volatile organic gas substances except carbon monoxide, carbon dioxide, carbonic acid and carbonate, including benzenes, alkanes, aromatics, alkenes, halocarbons, esters, aldehydes, ketones, etc. Formaldehyde is a kind of TVOC. The scope of TVOC is very wide, and there are many sources. Outdoor air pollutants caused by fossil fuel combustion, indoor furniture, paints, coatings, panels, etc. are also the main sources, and the harm to the human body is also numerous. Excessive TVOC will cause the immune level of the body to be imbalanced, and affect the function of the central nervous system, causing dizziness, headache, lethargy, weakness, chest tightness and other subjective symptoms. It may also affect the dig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16B01D53/84B01D53/44C12R1/225C12R1/72C12R1/46C12R1/23C12R1/245C12R1/24C12R1/73C12R1/74C12R1/01
CPCB01D53/44B01D53/84B01D2257/708C12N1/16C12N1/20Y02A50/20
Inventor 侯希波
Owner 侯希波
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