A method for simultaneous quantitative detection of astragaloside IV and cycloastragenol in mouse plasma

A technology of astragaloside IV and cycloastragenol, which is applied in the field of medicine, can solve problems such as low solubility, low bioavailability, and unsuitability, and achieve high specificity effects

Active Publication Date: 2021-04-06
MINZU UNIVERSITY OF CHINA
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  • Description
  • Claims
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Problems solved by technology

The other method takes a long data acquisition time (17 minutes)
In addition, the sample volume of the above two methods is 50 μL plasma, which is not suitable for quantification in mouse plasma
In addition, due to the high relative molecular mass and low solubility of AST, the bioavailability in animals is not high

Method used

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  • A method for simultaneous quantitative detection of astragaloside IV and cycloastragenol in mouse plasma
  • A method for simultaneous quantitative detection of astragaloside IV and cycloastragenol in mouse plasma
  • A method for simultaneous quantitative detection of astragaloside IV and cycloastragenol in mouse plasma

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Experimental program
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Embodiment

[0052] 1.1 Experimental reagents

[0053] Astragaloside IV (Astragaloside IV, AST), molecular formula C 41 h 68 o 14 , with a relative molecular mass of 784.4609, purchased from China National Institute for the Control of Pharmaceutical and Biological Products; cycloastragenol (Cycloastragenol, CST), molecular formula C 30 h 50 o 5 , relative molecular mass 490.3658, internal standard Digoxin (Digoxin), molecular formula C 41 h 64 o 14 , with a relative molecular mass of 780.4296, all purchased from Chengdu Kangbang Biotechnology Co., Ltd., with a purity of >98% by HPLC, sealed and protected from light, and stored at 2-8°C. Acetonitrile, methanol, (chromatographically pure) were purchased from Merck, Germany, and formic acid (chromatographically pure) was purchased from Roe, USA. The experimental water was Wahaha purified water.

[0054] 2.2 Plasma sample collection

[0055] Twenty-four male KM mice (8 weeks old, weighing 35-40 g) were purchased from Beijing Weitong ...

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Abstract

The present invention establishes a simultaneous quantitative method in mouse plasma based on UPLC-HRMS for astragaloside IV and its main metabolite cycloastragenol. The quantification time of this method is 3 minutes, digoxin is used as the internal standard, and only 20 μL of mouse plasma is needed, which has the advantages of rapidity, high sensitivity and strong specificity. After protein precipitation, the sample is filtered using a phospholipid-free plate, which effectively reduces the matrix effect of phospholipid endogenous metabolites in plasma on the analyte. The ultra-high-efficiency C18 chromatographic column is used as the analytical column, and the two analytes and the internal standard are detected in the positive ion selected ion monitoring mode of the electrospray ion source. The linear range of the two analytes is 1-200ng / mL; the intra-day and inter-day precision is ≤8.6%, and the precision is ≤8.8%, which shows that the precision and accuracy of this method are good. This method was successfully applied to the study of pharmacokinetics of astragaloside IV in mice.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the establishment of a simultaneous quantitative method for astragaloside IV and cycloastragenol in mouse plasma based on UPLC-HRMS and the application of pharmacokinetics. Background technique [0002] Astragaloside-IV (Astragaloside IV, AST), as the main active ingredient of Chinese herbal medicine Astragalus, has various pharmacological effects, including anti-inflammatory, anti-hypertensive, cardioprotective, anti-oxidative and anti-apoptotic. AST has also been reported as a potential therapeutic drug for various metabolic syndromes. The main bioactive metabolite of AST is Cycloastragenol (CST), which is a small molecule telomerase activator and a potential inhibitor of adipogenesis. In addition, recent studies have shown that AST and CST have equal efficacy in inhibiting reactive oxygen species (Reactive oxygen species, ROS)-related endoplasmic reticulum stress and inhib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7266
Inventor 王中华何秉淑陈路路再帕尔·阿不力孜
Owner MINZU UNIVERSITY OF CHINA
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