Microbial fruit preservative
A preservative and microbial technology, applied in the biological field, can solve problems such as unsatisfactory effects
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Embodiment 1
[0025] A preparation method of microbial fruit preservative, the steps are as follows:
[0026] (1) Fermentation of Bacillus licheniformis ZJU-X1:
[0027] Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerin 15.0ml, agar 15.0g, cornstarch 2-3g, stachyose 2-4g distilled water to volume To 1.0L; pH 7.0, 121°C, 15min sterilization;
[0028] Bacillus licheniformis ZJU-X1 was inoculated into LB medium according to the inoculum size of 10%, and fermented at 37-39°C for 3-7 hours to obtain preservative 1;
[0029] (2) Fermentation of Bacillus subtilis strain xp:
[0030] Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerol 15.0ml, agar 15.0g, cornstarch 2-3g, trehalose 2-4g, vitamin C0. 5g, distilled water to 1.0L; pH 7.0, sterilized at 121°C for 15 minutes;
[0031] Inoculate the Bacillus subtilis strain xp into LB medium according to the inoculum size of 10%, and ferment at 37-3...
Embodiment 2
[0036] A preparation method of microbial fruit preservative, the steps are as follows:
[0037] (1) Fermentation of Bacillus licheniformis ZJU-X1:
[0038] Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerin 15.0ml, agar 15.0g, cornstarch 2-3g, stachyose 2-4g distilled water to volume To 1.0L; pH 7.0, 121°C, 15min sterilization;
[0039] Bacillus licheniformis ZJU-X1 was inoculated into LB medium according to the inoculum size of 10%, and fermented at 37-39°C for 3-7 hours to obtain preservative 1;
[0040] (2) Fermentation of Bacillus subtilis strain xp:
[0041] Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerol 15.0ml, agar 15.0g, cornstarch 2-3g, trehalose 2-4g, vitamin C0. 5g, distilled water to 1.0L; pH 7.0, sterilized at 121°C for 15 minutes;
[0042] Inoculate Bacillus subtilis strain xp into LB medium according to 10% inoculum amount, ferment at 37-39°C for 1-3 h...
Embodiment 3
[0047] A preparation method of microbial fruit preservative, the steps are as follows:
[0048] (1) Fermentation of Bacillus licheniformis ZJU-X1:
[0049] Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerin 15.0ml, agar 15.0g, cornstarch 2-3g, stachyose 2-4g distilled water to volume To 1.0L; pH 7.0, 121°C, 15min sterilization;
[0050] Bacillus licheniformis ZJU-X1 was inoculated into LB medium according to the inoculum size of 10%, and fermented at 37-39°C for 3-7 hours to obtain preservative 1;
[0051] (2) Fermentation of Bacillus subtilis strain xp:
[0052]Configure LB medium (both mass concentration g / L): peptone 20g, K2HPO4.3H2O 2.5g, MgSO40.73g, glycerol 15.0ml, agar 15.0g, cornstarch 2-3g, trehalose 2-4g, vitamin C0. 5g, distilled water to 1.0L; pH 7.0, sterilized at 121°C for 15 minutes;
[0053] Inoculate the Bacillus subtilis strain xp into LB medium according to the inoculum size of 10%, and ferment at 37-39...
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