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A kind of dark septate endophyte fungus on blueberry roots and application thereof

A dark-colored endogenous, blueberry technology, applied in the application field of microbial technology, can solve the problems of being susceptible to adversity stress, lack of flora, slender and underdeveloped blueberry root system, etc., to promote blueberry growth, promote growth, and strengthen plants. The effect of disease resistance

Active Publication Date: 2022-02-01
ANQING NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the blueberry root system is slender and underdeveloped, without root hairs, vulnerable to adversity stress, and most blueberry cultivation areas lack the corresponding flora

Method used

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  • A kind of dark septate endophyte fungus on blueberry roots and application thereof
  • A kind of dark septate endophyte fungus on blueberry roots and application thereof
  • A kind of dark septate endophyte fungus on blueberry roots and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] Further, the preparation method of the fermentation filtrate of the blueberry DSE bacterial strain comprises: the blueberry DSE bacterial strain is cultivated on the PDA medium for two weeks, gets two bacterium cakes (7mm), adds 70ml liquid PDA medium, 25 ℃ 150r / min, shakes Bacteria 2 weeks to make fermentation broth.

[0043] Further, the blueberry DSE strain uses the plate confrontation method to antagonize blueberry pathogenic bacteria or antagonize Sclerotinia sclerotiorum. For example, use a hole puncher with an inner diameter of 6mm to punch out bacteria cakes at the edge of blueberry pathogenic bacteria and blueberry DSE strains, and put them into a petri dish with a diameter of 9cm. The distance between the two cakes and the edge of the petri dish is 3cm. Culture at constant temperature for 5-7 days.

[0044] Further, the 836bp 28s nrDNA sequence of the Pezicula sp.FLR13 is shown in the sequence listing SEQ ID No.1.

[0045]Further, the 499bp ITS sequence of P...

Embodiment 1

[0047] Example 1 Isolation and identification of FLR13

[0048] 1. Isolation and purification of blueberry endophytic fungi

[0049] The test material is the 15-year-old blueberry variety "Fenlan". Randomly select 3 trees, take samples from the east and west directions, remove the 5cm-thick topsoil and collect 5-20cm-deep fibrous roots connected to the main root, then put them together with the soil in a ziplock bag, put them in a refrigerator at 4°C and store them at Processed within 24 hours. Randomly select 2 cm root samples from each plant sample, rinse them with tap water, rinse with 75% ethanol for 1 min, rinse with sterile water for 3 times, disinfect with 0.1% mercury liter for 5 to 10 min, rinse with sterile water for 5 times, place in sterile On the filter paper, remove both ends of the root, cut the root sample into 5mm-sized tissue pieces, put them into culture dishes, 4-5 pieces per dish, randomly select 100-150 tissue pieces, and culture at a constant temper...

Embodiment 2

[0067] Example 2 Morphological and molecular biological identification of FLR13

[0068] 1. Morphological identification FLR13 bacteria cake with a diameter of 5mm was placed on a PDA plate and incubated at a constant temperature of 26°C for two weeks. The bacterial strain grows slowly, cultivated for two weeks, and the colony diameter is 22.4 ± 2.2cm, white, and does not produce spores ( figure 2 A). The morphological characteristics of the hyphae were observed under the microscope. The hyphae of FLR13 were hyphae with septa. When the hyphae grew to a certain stage, they produced protrusions, invaded adjacent hyphae, and mycelium fusion occurred. The content of the cells increased, and the hyphae became thicker ( figure 2 B). At the same time, the hyphae are intertwined into a rope shape, cell fusion occurs continuously around the rope-like hyphae, and the mycelium knot increases and thickens ( figure 2 C).

[0069] 2. Molecular biological identification

[0070] G...

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Abstract

The invention provides a dark-colored septated endophytic fungus on blueberry roots and its application, belonging to the field of microbial technology applications. The blueberry root dark septal endophyte (DSE) strain is specifically Pezicula sp.FLR13, and the preservation number is GDMCC No.60777. The strain has potential application value in promoting blueberry growth and enhancing plant disease resistance in blueberry cultivation, and at the same time, the strain promotes freshness of blueberry fruit. In addition, the strain can antagonize Sclerotinia sclerotiorum, and has a good effect on promoting tomato seed germination and root growth.

Description

technical field [0001] The invention belongs to the field of microbial technology application, and in particular relates to a dark-colored septated endophytic fungus on blueberry roots and its application. Background technique [0002] Blueberry has another name called lingonberry, belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium), is a kind of emerging small berry tree species with high economic value and broad development prospects in the world. Blueberry is a shallow-rooted plant with no root hairs. It is a typical host plant for heather mycorrhizal fungi (ericoid mycorrhiza, ERM). (arbuscular mycorrhiza, AM) infection and colonization. [0003] DSE is a small soil fungus that colonizes in plants, and generally exists in the cells or intercellular spaces of the epidermis and cortex of healthy plant roots. Colonies of DSE cultures can vary in color, from dark brown to light yellow, and even white. The two typical characteristics of DSE formed in the root system of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P21/00A23B7/155C12R1/645
CPCA23B7/155C12R2001/645C12N1/145
Inventor 宋晓贺李彦凯于道平尹立伟宋亚玲王慧丽
Owner ANQING NORMAL UNIV
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