A needle for mouse tail vein injection and its manufacturing method and experimental application

Abstract

The present invention relates to the technical field of using experimental mice to do in vivo experiments, and specifically discloses a needle for tail vein injection of mice and its manufacturing method and experimental application. Intravenous injection. The steps are as follows: on the basis of the existing 25G syringe needle for medical use, from the middle point of the needle downward, add left and right symmetrical flanks to make a new type of needle. Using this new type of needle for tail vein injection of mice can greatly improve the success rate of mouse tail vein injection; the advantage of the present invention is: compared with traditional medical needles of 25G syringes, using this new type of needle for tail vein injection of mice Intravenous injection experiments can effectively control the angle and length of the needle insertion, and avoid the needle from being stuck or piercing the tail vein due to incorrect needle insertion angle to the greatest extent.

Description

technical field [0001] The invention relates to the technical field of using experimental mice for in vivo experiments, in particular to a needle for tail vein injection of mice, a manufacturing method and experimental application thereof. Background technique [0002] Experimental mice are small in size, not aggressive, and easy to feed and operate. They are one of the most commonly used experimental animals for in vivo experiments. They are often used in experiments such as pharmacology, toxicology, tumors, immunity, and biochemistry. The experimental methods of mice mainly include intragastric administration, intraperitoneal injection, subcutaneous injection, tail vein injection, etc. Among them, tail vein injection is an important and commonly used experimental method. However, because the tail vein of the mouse is thin, it is difficult to puncture. During the injection process, the mouse twisted its body due to pain, causing the needle to slip out or puncture the tail v...

AI Technical Summary

Problems solved by technology

Advantages of this method: there is no need to purchase a mouse fixation device, saving costs; Disadvantages: During the process of puncturing and pushing the needle, the mouse tends to twist its body, resulting in unstable needle fixation, which can easily slip out or puncture the tail vein

Advantages of this method: The body parts other than the tail of the mouse are relatively fixed, which reduces the range of its twisting body to a certain extent, which is beneficial to puncture and needle pushing; Disadvantage: Depending on the number of experimental mice, one or more commercial mice need to be purchased for fixation device, increasing the cost of experiments

[0004] The above two conventional mouse tail vein injection methods have both advantages and disadvantages, but the goal is to successfully complete the tail vein injection operation of each experimental mouse in order to obtain real and effective in vivo experimental data

Images