A kind of dye compound and its preparation method and use

A compound and enzyme synthesis technology, applied in the field of dye compounds, can solve the problems of high production cost, limited content, high biological toxicity, etc., and achieve the effects of operation process control, low production cost and easy operation process

Active Publication Date: 2021-09-17
徐州合谷生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural dyes have been used earlier, and most of them are non-toxic and environmentally friendly, but there are limitations such as limited content in nature, complicated extraction process, and high production cost.
Compared with natural dyes, synthetic dyes have the advantages of bright color, not limited by the source of raw materials, and low production cost, but the disadvantages are that most of the synthetic dyes have complex structures, high biological toxicity, and are difficult to degrade or costly to degrade in nature. Great environmental damage
The above-mentioned shortcomings of natural dyes and synthetic dyes have seriously restricted the rapid development of the dye industry and the further expansion of its application fields.

Method used

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  • A kind of dye compound and its preparation method and use
  • A kind of dye compound and its preparation method and use
  • A kind of dye compound and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation method of 2-phenylacetyl-benzimidazole-7-carboxylic acid.

[0045] (1) Experimental materials

[0046] Fusarium graminearum PH-1 was purchased from the American Standard Biological Collection (ATCC), and its number was ATCC MYA-4620; the fungal genome DNA extraction kit and pYES2 plasmid vector were purchased from Thermo Fisher Scientific (China ) Co., Ltd.; restriction endonucleases BamHI and EcoRI were purchased from Bao Biological Engineering (Dalian) Co., Ltd.; primers A1 and A2 were ordered from Sangon Bioengineering (Shanghai) Co., Ltd.

[0047] (2) Preparation of engineering bacteria S (Saccharomyces cerevisiae).

[0048](1) Purify the genomic DNA of Fusarium graminearum PH-1 of strain number ATCC MYA-4620 with a fungal genomic DNA extraction kit;

[0049] (2) Using the purified genomic DNA of Gibberella maize as a template, use primer A1: 5'-CGGGATCCCCTCAGCGCCTGTACAAAGACAC-3' and primer A2: 5'-CGGAATTCCTAGTCATCCTGTAAGCTGATGGTC-3' to amplify 2-p...

Embodiment 2

[0074] Verification of the StBI gene enzyme for the synthesis of 2-phenylacetyl-benzimidazole-7-carboxylic acid.

[0075] 1. Transform the blank plasmid pYES2 without the inserted enzyme gene StBI into Saccharomyces cerevisiae engineering strain INVSc1, cultivate it in the same way as in Example 1 for 72 hours, and take the fermentation broth for later use.

[0076] 2. Get the fermentation liquid of the engineering bacteria S (Saccharomyces cerevisiae) of Example 1 and the above-mentioned fermentation liquid, and carry out liquid phase detection, 380nm wavelength, and the elution method is gradient elution for 35min, 30-90% (acetonitrile: water).

[0077] The result is as Figure 4 Shown, the fermented liquid that does not have the blank plasmid pYES2 of insertion enzyme gene StBI to be transformed into Saccharomyces cerevisiae engineering bacterium INVSc1 is shown in (I), shows that 2-phenylacetyl-benzimidazole-7-carboxylic acid is not detected; The Saccharomyces cerevisiae ...

Embodiment 3

[0079] Engineering bacteria S (Saccharomyces cerevisiae) prepared by the method of Example 1, as the preparation method of 2-phenylacetyl-benzimidazole-7-carboxylic acid in Example 1, engineering bacteria S (Saccharomyces cerevisiae) was fermented and cultivated for 12 hours respectively Hours, 24 hours, 36 hours, 48 ​​hours, 60 hours, 72 hours, 84 hours, 96 hours, 108 hours, 120 hours, as a result, 0.31g to 1.98g of 2 -Phenylacetyl-benzimidazole-7-carboxylic acid, such as Figure 6 shown.

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Abstract

The invention provides a compound, namely 2-phenylacetyl-benzimidazole-7-carboxylic acid, the structure of which is shown in formula I. The 2-phenylacetyl-benzimidazole-7-carboxylic acid of the present invention is obtained by engineering bacteria Synthesized by S (Saccharomyces cerevisiae), 2-phenylacetyl-benzimidazole-7-carboxylic acid has hydrophobic and oleophobic properties. It is a yellow pigment used for the preparation of dyes. Discoloration on contact with oil. Moreover, the synthesis method and the extraction process thereof have high dye output, are not limited by the source, are easy to control the operation process, and have low production cost.

Description

technical field [0001] The invention relates to the field of dye compounds, in particular to a dye compound and its preparation method and application. Background technique [0002] In recent years, with the advancement of technology and the improvement of people's living standards, the application of dyes has become more and more extensive, especially the demand for non-toxic and environmentally friendly dyes is increasing, which puts forward higher requirements for the development of the dye industry . Current dyes can be divided into two categories according to their sources: natural dyes and synthetic dyes. Natural dyes have been used earlier, and most of them are non-toxic and environmentally friendly, but there are limitations such as limited content in nature, complicated extraction process, and high production cost. Compared with natural dyes, synthetic dyes have the advantages of bright color, not limited by the source of raw materials, and low production cost, bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D235/12C09B23/16C12P17/10
CPCC07D235/12C09B23/166C12P17/10
Inventor 孙磊
Owner 徐州合谷生命科技有限公司
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