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Microcapsules for preventing soil caking as well as preparation method and application of microcapsules

A technology of soil compaction and microcapsules, which is applied in the fields of application, soil preparation methods, soil conditioning materials, etc., can solve the problems that it is difficult to effectively function, and the stability of microorganisms is easily affected by the soil or weather environment, so as to achieve stable properties and prevent soil compaction , the effect of increasing the content of available phosphorus

Inactive Publication Date: 2018-12-28
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If several kinds of phosphorus-solubilizing bacteria are directly and simply put into the soil, the stability of the microorganisms is easily affected by the soil or weather environment, and it is difficult to effectively function

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0028] The present invention provides the preparation method of above-mentioned microcapsule, comprises the steps:

[0029] (1) mixing the bacterium solution of phosphobacteria, sodium alginate solution and chitosan solution in a volume ratio of 4 to 8:1.5 to 2.5:1 to obtain a mixed solution of capsule core solution and sodium alginate solution;

[0030] (2) The mixed solution of the capsule core solution and the sodium alginate solution is dropped into the calcium chloride solution, and the sodium alginate reacts with the calcium chloride to generate the capsule calcium alginate rubber ball, the bacterium solution of the phosphobacteria and the chitosan The sugar solution is wrapped in sodium alginate rubber balls to obtain microcapsules;

[0031] In the invention, the bacterium liquid of phosphate solubilizing bacteria, the sodium alginate solution and the chitosan solution are firstly mixed to obtain the mixed solution of the capsule core solution and the sodium alginate so...

Embodiment 1

[0049] (1) Prepare the bacterial suspensions of Staphylococcuspasteuri bacteria (bacteria a) and Pseudomonasfluorescens Migula bacteria (bacteria b), and measure the effective concentration of viable bacteria as bacteria a, 5~8×10 9 cfu / ml; Bacteria b, 2~4×10 9 cfu / ml. Then the suspension of bacteria a and the suspension of bacteria b were mixed according to the volume of 1:1 to obtain the composite bacteria liquid (the total concentration of effective viable bacteria was about 4~6×10 9 cfu / ml).

[0050] (2) Mix the chitosan solution with the 2.0% mass concentration sodium alginate of the composite bacterial liquid and the sterilized 0.6% mass concentration after the sterilization with a volume ratio of 6:2:1, and place it on the ultra-clean workbench Drop the above mixed solution into the sterilized 1% mass concentration of CaCl 2 solution to produce microcapsules.

[0051] (3) Place the prepared microcapsules at 20°C for 2 hours, then wash them with sterile water for 4 t...

Embodiment 2

[0054] (1) Prepare the bacterial suspensions of Staphylococcuspasteuri bacteria (bacteria a) and Pseudomonasfluorescens Migula bacteria (bacteria b), measure the effective concentration of viable bacteria as bacteria a, 2~4×10 8 cfu / ml; Bacteria b, 2~4×10 8 cfu / ml. Then the suspension of bacteria a and the suspension of bacteria b were mixed according to the volume of 2:1 to obtain the composite bacteria liquid (the total concentration of effective viable bacteria was about 2~4×10 8 cfu / ml).

[0055] (2) Mix the chitosan solution with the 1.5% mass concentration of sodium alginate after the composite bacteria solution and the sterilized 0.8% mass concentration with a volume ratio of 6:2:1, and put it on the ultra-clean workbench Drop the above mixed solution into the sterilized 1% mass concentration of CaCl 2 solution to produce microcapsules.

[0056] (3) Place the prepared microcapsules at 20°C for 2 hours, then wash them three times with sterile water, soak the microcap...

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Abstract

The invention provides microcapsules for preventing soil caking as well as a preparation method and an application of the microcapsules and belongs to the technical field of soil treatment and remediation. Each microcapsule comprises a capsule body and a capsule core, wherein the capsule body is a calcium alginate gel ball generated through a reaction of a sodium alginate solution and a calcium chloride solution; the capsule core is a mixed solution of a phosphate solubilizing bacteria solution and a chitosan solution; the concentration of effective active bacteria of the phosphate solubilizing bacteria solution is 107-1010 cfu / ml; the mass concentration of the chitosan solution is 0.5%-1.0%; the volume ratio of the phosphate solubilizing bacteria solution to the chitosan solution is (4-8):1. The microcapsules have good functions of promoting phosphorus circulation, stabilizing soil granulation structures, increasing the content of available phosphorus and preventing soil caking. Meanwhile, with the application of a microcapsule technology, the stability and the effectiveness of a microbial agent can be effectively maintained.

Description

technical field [0001] The invention belongs to the technical field of soil treatment and restoration, and in particular relates to a microcapsule for preventing soil compaction, a preparation method and application thereof. Background technique [0002] With the continuous development of agricultural production activities, different degrees of soil compaction have occurred in cultivated land in various parts of my country, especially in the dry North China region. The compacted soil retains water, fertilizer and permeability, which has gradually become a constraint to the sustainable development of rural productivity. Key factor. Improper use of chemical fertilizers is an important cause of soil compaction. In order to increase the available phosphorus content in the soil and promote plant growth, a large amount of phosphorus fertilizers are often applied during agricultural production practices. Since the seasonal utilization rate of phosphorus fertilizers usually does not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/32C09K17/16A01B79/02C09K101/00
CPCA01B79/02C09K17/16C09K17/32C09K2101/00
Inventor 刘志伟张雯连世举宋子谦罗明明王新剑丁云鹏张振
Owner YANSHAN UNIV
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