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High-temperature-resistant cellulose degraded bacilli and application thereof

A technology of cellulose degradation and bacillus, applied in the direction of application, bacteria, microorganisms, etc., to achieve the effect of good degradation ability

Inactive Publication Date: 2018-12-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few studies have screened high-temperature cellulose-degrading strains from protists in swine manure fermentation bed habitats.

Method used

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  • High-temperature-resistant cellulose degraded bacilli and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Isolation, purification and preservation of strains

[0020] Take the naturally fermented pig manure sawdust from the pig farm of Zhejiang Lishui Yixin Animal Husbandry Co., Ltd. as a sample, take 10g and put it in 100mL enrichment medium, and cultivate it at a constant temperature of 50°C and 150rpm. The enrichment medium uses CMC-Na as the only carbon source. Take 100ul of the enrichment solution and spread it on the CMC-Congo red medium plate, and then culture it upside down in a 50°C incubator at a constant temperature. Observe after 5 days that a transparent hydrolysis circle appears on the plate. Pick a small amount of bacteria and streak on LB solid medium until a single colony is obtained, and then continuously culture the obtained single colony on the medium with CMC-Na as the only carbon source for 6 generations. After the colony form is stable, you can get Pure culture Bacillus subtilis SM-5-1 (Bacillus Subtilis SM-5-1), the morphology is as follows fig...

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Abstract

The invention discloses high-temperature-resistant cellulose degraded bacilli named as bacillus subtilis SM-5-1(bacillus Subtilis SM-5-1) and preserved in CCTCC on July 15, 2018, with the preservationnumber of CCTCC No:M2018415. The high-temperature-resistant cellulose degraded bacilli have good degradation capacity on cellulose, products cannot pollute environment, the high-temperature-resistantcellulose degraded bacilli are free from pig manure and used as aboriginal bacteria of the pig manure, can reduce the restraining effect with aboriginal bacteria, have high degradation capacity on compost of the pig manure and a fermentation bed, besides, have high temperature tolerance, can be made into addition bacteria of a thermophilic fermentation bed, and have application prospects in the field of fermentation beds of the pig manure.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a strain of high-temperature-resistant cellulose-degrading bacillus and its application. Background technique [0002] Straw lignocellulose is the most effective and renewable resource on earth, and it is widely used in the production of various agricultural and sideline products. As a large grain-producing country, China produces nearly 700 million tons of crop straws every year. The problem of straw disposal is prominent. Most of the straws are burned or dumped randomly, which not only wastes precious renewable resources, but also brings various hazards. Cause air pollution, soil mineralization, fire and traffic accidents. [0003] The comprehensive utilization of straw resources has become a hot spot that the society pays close attention to. Common treatment methods for lignocellulose include physical, chemical, and biological methods, among which biological methods hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/125
CPCC05F5/002C05F17/00C12N1/205C12R2001/125C05F11/00C05F11/08Y02W30/40
Inventor 沈琦姚晓红汤江武吴逸飞孙宏李园成王新
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES