Rapid, efficient and transplantable identification method for salt-tolerant corn germplasm

Abstract

The invention discloses a rapid, efficient and transplantable identification method for salt-tolerant corn germplasm. The method comprises the following steps: germinating corn inbred line seeds; placing the germinated seeds on a transplanting discs for storage; putting the plurality of transplanting discs into different containers respectively, presetting a gap between the bottom of each transplanting disc and the inner wall of the bottom of one corresponding container in height, and injecting a corn water culture nutrient solution into the containers; adding salt into the nutrient solutionsin a part of the containers while correspondingly salt is not added into the nutrient solutions in other containers as control; removing corn endosperm after corn cotyledons are completely unfolded; and carrying out grading identification and screening on a germplasm material. The germplasm material screening is carried out by adopting the following processing mode: firstly, a mutant material is obtained through screening, transplanting is carried out to ensure normal pollination and fructification when salt-resistant single plants appear in the salt-sensitive material, and genetic research ofexcellent gene resource mining and the like is carried out; and secondly, integral transplantation is carried out on the stable salt-resistant material, and salt resistance experiment research or breeding research is carried out. The method can be used for simply, rapidly, efficiently identifying salt-resistant corn germplasm and transplanting the salt-resistant corn germplasm without damage.

Description

technical field [0001] The invention relates to the technical field of corn salt tolerance test and identification method, in particular to a fast, efficient and transplantable identification method for salt-tolerant corn germplasm. Background technique [0002] Soil salinization is the main restrictive factor for agricultural production in coastal areas and semi-arid areas. The main feature of saline-alkali land is that it is prone to salt spots, irregular and uneven. Maize is the largest food crop in my country, and it is a dryland food and feed crop with wide adaptability and high yield potential. At present, there is a shortage of salt-tolerant and high-yielding maize varieties. The main limiting factor is the lack of maize germplasm, especially the lack of salt-tolerant maize germplasm materials. There are few salt-tolerant mutants obtained through internal variation screening, and there is a lack of systematic mechanism research. . At present, the means of studying m...

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Problems solved by technology

[0003] The purpose of the present invention is to solve the above-mentioned problems existing in the prior art, to provide a fast, efficient, and transplantable identification method for salt-tolerant corn germplasm, and to conduct research on the mining and utilization of excellent genetic resources directly on the mutant materials obtained through screening, Carry out genetic research or breeding research on the salt-tolerant inbred lines obtained through screening; the present invention can simply, quickly and efficiently initially identify and evaluate the salt-tolerant corn germplasm, and carry out non-damage transplanting for utilization; in addition, the present invention removes the endosperm In this way, the impact of seed nutrients on the screening and identification of salt tolerance can be reduced, and the efficiency and accuracy of corn salt tolerance screening and identification can be greatly improved. shortcoming

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