Modeling method for irritable bowel syndrome

Abstract

The invention discloses a modeling method of irritable bowel syndrome. The method comprises the steps of using a balloon dilatation catheter to perform colonic expansion stimulation on newborn rats of8-21 days old, giving a pressure of 60 mmHg in the rectum for 1 min, and repeating the stimulation after 30 min. The abdominal wall retraction reflex test scoring is performed 90 days after birth, aresult that the AWR score of the model rats is significantly higher than that of a normal control group is obtained, and the result proves that the visceral hypersensitivity model is replicated successfully and continued to the adult stage of the rates, and histopathological examination, colon tissue SP immunohistochemical examination and other related factors demonstrate successful modeling of the method. The model of irritable bowel syndrome provided by the invention can be used for screening drugs for controlling irritable bowel syndrome, has good stability and is consistent with human IBSsymptoms, and thus can simulate a long-term chronic course of IBS.

Description

technical field [0001] The invention relates to an animal model method, in particular to a modeling method for irritable bowel syndrome. Background technique [0002] Irritable bowel syndrome (IBS) is a heterogeneous disease characterized by intestinal dysfunction, and its main symptoms are recurrent abdominal pain, diarrhea, and abnormal bowel movements. In recent years, the prevalence of irritable bowel syndrome has been on the rise, and compared with diseases of the same level in adults such as diabetes, congestive heart failure, renal insufficiency, and cirrhosis, IBS has greatly affected the physical and mental health of patients and quality of life have become one of the clinical problems to be solved. [0003] IBS is a gastrointestinal functional disorder involving the abnormal interaction of multiple complex factors between the brain and the intestine, and its etiology and pathogenesis have not yet been clarified. Most scholars believe that increased visceral sensi...

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Problems solved by technology

[0004] The traditional IBS animal model replication methods mainly include restraint brake stimulation, acetic acid intestinal stimulation, electrical stimulation, and adult animal rectal expansion stimulation, etc., but the model is poor in repeatability, and intestinal paresthesia will disappear as the inflammation subsides , does not qualify as a chronic persistent course of abdominal pain or abdominal discomfort in IBS patients

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