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Extraction method of sea cucumber collagen fiber

A collagen fiber and extraction method technology, applied in the field of sea cucumber collagen fiber extraction, can solve the problems of containing other impurities, incomplete collagen fiber structure, difficult extraction, etc., and achieve the effects of avoiding degradation and damage, uniform diameter, and simple operation steps

Inactive Publication Date: 2019-01-04
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sea cucumber collagen fibers are in the form of a network, which is difficult to extract. The structure of collagen fibers extracted by existing methods is incomplete and contains other impurities, not pure collagen fibers.

Method used

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  • Extraction method of sea cucumber collagen fiber
  • Extraction method of sea cucumber collagen fiber
  • Extraction method of sea cucumber collagen fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] S1. Fresh sea cucumber pretreatment: remove the anus, mouth, tentacles, viscera and contractile muscles of the fresh sea cucumber, clean them, and cut the resulting body wall into about 4cm 3 Small pieces, put at 0 ℃ for later use;

[0042] S2, one-time water washing: take the small pieces of sea cucumber body wall obtained in step S1, add 2 times the weight of water at 0°C, use a beater at 18,000 rpm, 4s / time×3 times, and prepare sea cucumber pulp at an interval of 30s each time; The sea cucumber slurry was stirred at 0°C for 1 hour, centrifuged at 10,000×g for 5 minutes, and the precipitate was collected;

[0043] S3. Secondary water washing: Add 2 times the weight of 0°C water to the precipitate obtained in step S2, stir at 0°C for 1 hour, centrifuge at 10,000×g for 5 minutes, and discard the precipitate; the purpose of the above two steps S2 and S3 is to initially remove Water-soluble impurities in sea cucumber body wall;

[0044] S4. Buffer washing: Add 2 times t...

Embodiment 2

[0051] S1. Fresh sea cucumber pretreatment: remove the anus, mouth, tentacles, viscera and contractile muscles of the fresh sea cucumber, clean them, and cut the body wall of the sea cucumber into about 9cm 3 Small pieces, put at 4°C for later use;

[0052] S2. One-time water washing: take the small pieces of sea cucumber body wall obtained in step S1, add 10 times the weight of water at 4°C, use a homogenizer at 23,000 rpm, 10 s / time x 3 times, and prepare sea cucumber slurry at an interval of 30 s each time; The obtained sea cucumber slurry was stirred at 4°C for 3 hours, centrifuged at 15,000×g for 20 minutes, and the supernatant was discarded to obtain a precipitate;

[0053] S3. Second washing: Add 10 times the weight of water at 4°C to the precipitate obtained in step S2, stir at 4°C for 3 hours, centrifuge at 15,000×g for 20 minutes, discard the supernatant, and collect the precipitate; the above two steps of S2 and S3 The purpose is to initially remove water-soluble i...

Embodiment 3

[0061] S1. Fresh sea cucumber pretreatment: remove the anus, mouth, tentacles, viscera and contractile muscles of fresh sea cucumbers, clean them, and cut them into about 6cm 3 Small pieces, set aside at 3°C;

[0062] S2. One-time water washing: take the small piece of sea cucumber body wall obtained in step S1, add 6 times the weight of water at 3°C, 20000 rpm, 8s / time×3 times, and prepare sea cucumber slurry at an interval of 30s each time; the obtained sea cucumber slurry is prepared in 3 Stir at ℃ for 2 hours, centrifuge at 12800×g for 12 minutes, discard the supernatant to obtain a precipitate;

[0063] S3, secondary water washing: add 6 times the weight of 3°C water to the precipitate obtained in step S2, stir at 3°C ​​for 2 hours, centrifuge at 12800×g for 12 minutes, discard the supernatant, and collect the precipitate; the above two steps of S2 and S3 The purpose is to initially remove water-soluble impurities in the body wall of sea cucumbers;

[0064] S4. Buffer w...

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Abstract

The invention discloses an extraction method of sea cucumber collagen fiber. The extraction method includes: pretreating sea cucumber, washing with water for the first time, washing with water for thesecond time, washing with buffering liquid, pickling, washing with water for the third time, and extracting to obtain complete sea cucumber collagen fiber. Fresh sea cucumber is used as a raw material, so that original state of each structure in sea cucumber body wall is ensured; the sea cucumber collagen fiber with a complete supermolecular structure can be obtained after a series of impurity removal and extraction; each step is proceeded at 0-4 DEG C, so that degradation damage caused to the sea cucumber collagen fiber is avoided, and structural integrity of the sea cucumber collagen fiberis maintained to greatest extent; obtained rope-shaped fiber is orderly in structural arrangement, is uniform in diameter of about 2.27um and smooth in surface and has obvious periodic transverse grain structure. The extraction method is concise in step, simple in operation, good in extraction effect and great in popularization and application prospect.

Description

technical field [0001] The invention relates to the technical field of extracting collagen fibers, in particular to a method for extracting sea cucumber collagen fibers. Background technique [0002] Sea cucumber belongs to the phylum Echinodermata and the class Holothuria. It has the characteristics of high protein, low fat, and no cholesterol. In recent years, the output has gradually increased, and its nutritional and economic value is extremely high. The body wall of sea cucumber is the main edible part of sea cucumber. It belongs to variable collagen tissue, which contains basic structural units such as collagen, proteoglycan and glycoprotein. It is mainly composed of supramolecular structures such as collagen fibers and microfibers, forming a network structure. Sea cucumber collagen fibers are network-like and difficult to extract. The collagen fibers extracted by existing methods have incomplete structure and contain other impurities, and are not pure collagen fibers....

Claims

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Application Information

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IPC IPC(8): A23J1/04
CPCA23J1/04
Inventor 刘玉欣周大勇刘自强宋亮李德阳刘中原马倩茹
Owner DALIAN POLYTECHNIC UNIVERSITY
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