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Serum-free culture medium capable of promoting multipotential cells of peripheral blood to differentiate into cartilage cells

A technology of serum-free medium and pluripotent cells, which is applied in the field of stem cell differentiation and can solve the problems of low efficiency

Inactive Publication Date: 2019-01-04
丰泽康生物医药(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have used peripheral blood pluripotent cells, which have self-renewal ability and multiple differentiation potentials, and can be differentiated into various cells in vitro, including chondrocytes, but the current culture medium is in peripheral blood pluripotent cells. In the differentiation culture of chondrocytes, the efficiency is not high, therefore, a medium specially used for inducing the differentiation of peripheral blood pluripotent cells into chondrocytes is needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A serum-free medium for improving the differentiation of peripheral blood pluripotent cells to chondrocytes, including a basal medium, the basal medium is high-sugar DMEM culture fluid, and the following substances are added to the basal medium:

[0038] Transforming growth factor β1 at 10ng / ml;

[0039] Transforming growth factor β3 at 5ng / ml;

[0040] 70ng / ml insulin-like growth factor;

[0041] 0.5mg / L astragalus polysaccharide;

[0042] 5mg / L perilla seed extract;

[0043] 0.1ng / ml of kelp extract;

[0044] 8mg / L ginsenoside Rg1;

[0045] 6mol / L dexamethasone;

[0046] 37mg / ml of ascorbic acid;

[0047] Sodium pyruvate at 0.8 μmol / ml;

[0048] 6.5 μg / ml transferrin.

[0049] Wherein, the described astragalus polysaccharide is prepared by the following method: Astragalus is crushed to obtain Astragalus powder, water with 4 times the weight of Astragalus powder is added, after boiling, it is kept for 40 minutes, and filtered to obtain the filtrate and the init...

Embodiment 2

[0059] A serum-free medium for improving the differentiation of peripheral blood pluripotent cells to chondrocytes, including a basal medium, the basal medium is high-sugar DMEM culture fluid, and the following substances are added to the basal medium:

[0060] Transforming growth factor β1 at 17ng / ml;

[0061] Transforming growth factor β3 at 9 ng / ml;

[0062] 68ng / ml insulin-like growth factor;

[0063] 0.7mg / L astragalus polysaccharide;

[0064] 3mg / L perilla seed extract;

[0065] 0.2ng / ml of kelp extract;

[0066] 6mg / L ginsenoside Rg1;

[0067] 8mol / L dexamethasone;

[0068] 38mg / ml of ascorbic acid;

[0069] 1.2 μmol / ml of sodium pyruvate;

[0070] 6.5 μg / ml transferrin;

[0071] 6mg / ml selenic acid;

[0072] 5mg / ml of linoleic acid;

[0073] 1mg / ml bovine serum albumin;

[0074] 20 mg / ml proline.

[0075] Wherein, the astragalus polysaccharide is prepared by the following method: Astragalus is pulverized to obtain Astragalus powder, water with 5 times the wei...

Embodiment 3

[0085] A serum-free medium for improving the differentiation of peripheral blood pluripotent cells to chondrocytes, including a basal medium, the basal medium is high-sugar DMEM culture fluid, and the following substances are added to the basal medium:

[0086] Transforming growth factor β1 at 10ng / ml;

[0087] Transforming growth factor β3 at 10ng / ml;

[0088] 60ng / ml insulin-like growth factor;

[0089] 0.8mg / L astragalus polysaccharide;

[0090] 5mg / L perilla seed extract;

[0091] 0.4ng / ml of kelp extract;

[0092] 10mg / L ginsenoside Rg1;

[0093] 1mol / L dexamethasone;

[0094] 38mg / ml of ascorbic acid;

[0095] Sodium pyruvate at 0.8 μmol / ml;

[0096] 6.5 μg / ml transferrin;

[0097] 10mg / ml selenic acid;

[0098] 5mg / ml of linoleic acid;

[0099] 5mg / ml bovine serum albumin;

[0100] 10 mg / ml proline.

[0101] Wherein, the astragalus polysaccharide is prepared by the following method: astragalus powder is obtained by crushing astragalus, adding water with 3 ti...

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Abstract

The invention belongs to the technical field of stem cell differentiation, and particularly relates to a serum-free culture medium capable of promoting multipotential cells of peripheral blood to differentiate into cartilage cells. The serum-free culture medium comprises a basal culture medium with following additions: a transforming growth factor Beta 1, a transforming growth factor Beta 3, an insulin-like growth factor, astragalus polysaccharide, purple perilla seed extract, ecklonia cava extract, ginsenoside Rg1, dexamethasone, ascorbic acid, sodium pyruvate and transferrin. The serum-freeculture medium has the advantages that various growth factors and various extracts are added in cell culturing liquid, the ingredients in the cell culturing liquid are reasonably combined and can synergize to promote and induce the multipotential cells of the peripheral blood to differentiate into the cartilage cells, the amount of the multipotential cells of the peripheral blood, differentiatinginto the cartilage cells, is increased, and differentiating time is shortened.

Description

technical field [0001] The invention belongs to the technical field of stem cell differentiation, and in particular relates to a serum-free medium for improving the differentiation of peripheral blood pluripotent cells into chondrocytes. Background technique [0002] Using chondrocytes to repair damaged cartilage tissue is a new technology used in modern medicine to replace traditional treatment methods. The sources of chondrocytes are constantly expanding in research. At present, some studies use peripheral blood pluripotent cells, which have self-renewal ability and multiple differentiation potentials, and can be differentiated into various cells in vitro, including chondrocytes, but the current culture medium is in peripheral blood pluripotent cells. In the culture of differentiation into chondrocytes, the efficiency is not high, therefore, a medium specially used for inducing the differentiation of peripheral blood pluripotent cells into chondrocytes is needed. Conten...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2500/24C12N2500/30C12N2500/32C12N2500/36C12N2500/38C12N2500/76C12N2500/90C12N2501/105C12N2501/15C12N2501/855C12N2501/90C12N2501/998C12N2506/1369
Inventor 马亚东黄宗堂曹娜
Owner 丰泽康生物医药(深圳)有限公司
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