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Cholesterol extraction process

A cholesterol and process technology, applied in the field of cholesterol extraction process, can solve the problems of high cost and low extraction efficiency, and achieve the effects of low cost, thorough extraction and cost saving

Inactive Publication Date: 2019-01-11
四川奇格曼药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The technical problem solved by the present invention is that in the prior art, there are only different process routes for extracting cholesterol from lanolin and the process route for obtaining cholesterol by microbial fermentation, but the cost is high and the extraction efficiency is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] ① pulverize pig brain to obtain pig brain homogenate;

[0045] ②Add extraction solvent to pig brain homogenate: methanol:chloroform=2.3:1v / v, pig brain homogenate: extraction solvent=1:10.6 w / v, stir and extract at 0~30°C for 6 hours to obtain extract;

[0046] ③ filtering the extract, concentrating the filtered liquid to obtain a concentrated solution, and recovering the solvent at the same time;

[0047] ④ Add methanol solution containing 10v / v% concentrated sulfuric acid containing 10 times the mass of the concentrated solution to the concentrated solution, reflux and hydrolyze at 70°C for 24 hours, filter the refluxed liquid, and crystallize the filtered liquid at 0°C for 12~72h to obtain Crystals;

[0048] ⑤Filtrate the crystals in step ④, add methanol according to 4.2 times the mass of the crystals, raise the temperature to 70°C until the crystals dissolve, reflux at 70°C for 1 hour, place the refluxed liquid at 0°C, and crystallize for 12~72h;

[0049] ⑥Filtrate ...

Embodiment 2

[0053] ① pulverize pig brain to obtain pig brain homogenate;

[0054] ②Add extraction solvent to pig brain homogenate: methanol:ethanol=1~5:1v / v, pig brain homogenate:extraction solvent=1:10w / v, stir and extract at 0~30°C for 6 hours to obtain extract;

[0055] ③ filtering the extract, concentrating the filtered liquid to obtain a concentrated solution, and recovering the solvent at the same time;

[0056] ④ Add methanol solution containing 10v / v% concentrated sulfuric acid containing 10 times the mass of the concentrated solution to the concentrated solution, reflux and hydrolyze at 70°C for 24 hours, filter the refluxed liquid, and crystallize the filtered liquid at 0°C for 12~72h to obtain Crystals;

[0057] ⑤Filtrate the crystals in step ④, add methanol according to 4.2 times the mass of the crystals, raise the temperature to 70°C until the crystals dissolve, reflux at 70°C for 1 hour, place the refluxed liquid at 0°C, and crystallize for 12~72h;

[0058] ⑥Filtrate the c...

Embodiment 3

[0062] ① pulverize pig brain to obtain pig brain homogenate;

[0063] ②Add extraction solvent to pig brain homogenate, ethanol:chloroform=1~5:1v / v, pig brain homogenate:extraction solvent=1:10w / v, stir and extract at 0~30°C for 6 hours to obtain extract;

[0064] ③ filtering the extract, concentrating the filtered liquid to obtain a concentrated solution, and recovering the solvent at the same time;

[0065] ④ Add methanol solution containing 10v / v% concentrated sulfuric acid containing 10 times the mass of the concentrated solution to the concentrated solution, reflux and hydrolyze at 70°C for 24 hours, filter the refluxed liquid, and crystallize the filtered liquid at 0°C for 12~72h to obtain Crystals;

[0066] ⑤Filtrate the crystals in step ④, add methanol according to 4.2 times the mass of the crystals, raise the temperature to 70°C until the crystals dissolve, reflux at 70°C for 1 hour, place the refluxed liquid at 0°C, and crystallize for 12~72h;

[0067] ⑥Filtrate the...

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Abstract

The invention discloses a cholesterol extraction process including the following operations: (1) preparing a pig brain homogenate from a pig brain; (2) adding an extraction solvent into the pig brainhomogenate to obtain an extraction solution; (3) filtering the extraction solution, concentrating the filtered liquid to obtain a concentrated solution, and recovering the solvent; (4) adding a methanol solution to the concentrated solution, filtering a refluxed liquid and crystallizing for 12-72 h, to obtain crystals; (5) filtering the crystals in the step (4), adding methanol with the amount 4-7times of the mass of the crystals, refluxing for 1 h at the temperature of 70 DEG C, placing the refluxed liquid at the temperature of 0 DEG C, and crystallizing for 12-72 h; (6) filtering the crystals in the step (5), adding methanol with the amount 2-5 times of the mass of the crystals with the temperature of methanol of -30-0 DEG C, and washing to be neutral; (7) adding methanol with the amount 3.5-6 times of the mass of the neutral crystals of the step (6), and refluxing for 1 h at the temperature of 70 DEG C; and placing the refluxed solution at the temperature of 0 DEG C, crystallizingfor 12-72 h, and filtering the crystals; and (8) carrying out vacuum drying of the final crystals of the step (7) at the temperature of 70-80 DEG C, to obtain cholesterol. The method has high yield and high content.

Description

technical field [0001] The invention relates to the field of extraction, in particular to a process for extracting cholesterol. Background technique [0002] Cholesterol is also known as cholesterol, cholest-5-en-3β-ol, isooctenol, cholesteryl alcohol, cholestenol or cholesterol cholesterol, and its English name is Cholesterol, Cholest-5-en-3β -ol, Cholesterin, the molecular formula is C 27 h 46 O, molecular weight is 386.65, its structural formula is as follows: [0003] [0004] Molecular formula: C 27 h 46 o [0005] Molecular weight: 386.65 [0006] The structure of cholesterol [0007] Cholesterol is a white or light yellow pearly flaky crystal. It is the first steroid compound discovered. Its melting point is 148.5°C and its density is 1.067g / cm 3 , can be sublimated under high vacuum, slowly oxidize and turn yellow in air, and its melting point and solubility also change accordingly. Its monohydrate C 27 h 46 O-H 2 O is a small pearlescent liquid crystal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 姚静张宪胡伟杨琴飞李豫俭曾奇祥
Owner 四川奇格曼药业有限公司
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