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Kit containing CA8 gene and used for screening milk traits of Kazakh horse and application thereof

A technology of lactation traits and kits, which is applied in the identification field of animal genetic selection and breeding, can solve problems such as unseen research reports, and achieve the effects of increasing economic income, protecting resources of excellent varieties, and accelerating development

Active Publication Date: 2019-01-11
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been many studies on the functional genes of livestock milk production traits, but there are no relevant research reports on the CA8 gene as a candidate gene affecting the lactation traits of Kazakh horses

Method used

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  • Kit containing CA8 gene and used for screening milk traits of Kazakh horse and application thereof
  • Kit containing CA8 gene and used for screening milk traits of Kazakh horse and application thereof
  • Kit containing CA8 gene and used for screening milk traits of Kazakh horse and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: The present invention is used to detect the kit of lactation traits of Kazakh horses

[0050] The invention provides a detection kit for detecting the lactation traits of the Kazakh horse of the CA8 gene, wherein the allele types of the CA8 gene include AA, AG and GG.

[0051] In the present invention, allelic types AA, AG and GG were detected from Kazakh horse plasma.

[0052] The present invention also provides a detection kit containing the CA8 gene for screening the lactation traits of Kazakh horses, comprising a box body 1, a label 12 is arranged on the outside of the box body 1, and the inside of the box body 1 is divided into 96-well plate areas by partitions 3 in turn 31. Tip area 32, blood collection tube area 33, DNA extraction reagent area 34, PCR amplification reagent area 35 and Marker area 36, ​​96-well plate area 31, tip area 32, blood collection tube area 33, DNA extraction reagent area 34 , PCR amplification reagent area 35 and Marker are...

Embodiment 2

[0064] Embodiment 2: The present invention is used to detect the kit of Kazakh horse lactation traits

[0065] The present invention provides a detection kit containing CA8 gene for screening the lactation traits of Kazakh horses. The box body 1 is in the shape of a cylinder, and a central column 4 is arranged in the middle of the box body 1. A shaft hole 42 is arranged at the top of the central column 4, and the cover is divided into two parts. 2. A rotating shaft 41 adapted to the shaft hole 42 is provided at the bottom, so that the sub-cover 2 can be rotated and placed on other sub-covers 2 after opening, without occupying space. The partition 3 and the sub-cover 2 make each area airtight and divided , will not interfere with each other.

[0066] In the present invention, the 96-well plate area 31, the tip area 32, the blood collection tube area 33, the DNA extraction reagent area 34, the PCR amplification reagent area 35 and the Marker area 36 are all fan-shaped areas, and...

Embodiment 3

[0071] Example 3: Application of a detection kit containing CA8 gene for improving lactation traits of Kazakh horses

[0072] The present invention provides the application of the detection kit containing CA8 gene for improving the lactation traits of Kazakh horses, which is embodied by the detection method and specifically includes the following steps:

[0073] (1) Extraction of horse genomic DNA:

[0074] Use a blood collection tube filled with sodium citrate anticoagulant to collect 5ml of Kazakh horse jugular venous blood in vivo, use the DNA extraction reagent, use the kit provided in the above example to extract genomic DNA, store at -20°C for later use, and extract the genome DAN gel electrophoresis, as attached Figure 4 shown.

[0075] (2) CA8 gene PCR amplification:

[0076] According to the designed primers, the kit provided in the above examples was used to specifically amplify the CA8 gene; the PCR reaction system was 2×Master Mix 2.5 μl, each of the upstream a...

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Abstract

The invention discloses a kit containing CA8 gene and used for screening milk traits of Kazakh horse and an application thereof. The detection kit disclosed by the invention is utilized to breed and promote milk traits of Kazakh horse by detecting alleles AA, AG and GG. The detection kit is equipped with six zones, including a 96-pore plate zone, a tip zone, a blood sampling tube zone, a DNA extraction reagent zone, a PCR amplification reagent zone and a Marker zone which are all fan-shaped zones; PCR amplification is performed by taking a sample genome DNA as a template according to a specific primer; KASP parting is performed on a PCR amplification product. The kit disclosed by the invention is capable of supplying a molecule-assistant breeding theoretical basis and a technical support for breeding Kazakh horse species with excellent milk traits and has a practical application value in protecting superior variety resources of Kazakh horse, increasing economic income of farmers and herdsmen and accelerating development of horses for milk.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection, specifically, the invention relates to the technical field of a kit for screening lactation traits of Kazakh horses. Background technique [0002] Xinjiang's horse breeding industry has a long history and is famous for its rich production of fine horses. It is also one of the important horse production bases in my country. Xinjiang is rich in horse resources, and the number of horses in stock remains at about 890,000. There are a large number of horses and rich breed resources. Kazakh horses are herbivorous livestock. The Kazakh horse produced in Xinjiang is also a grassland horse breed. Its morphological characteristics are: the head is medium and large, delicate, and the ears are short. The neck is slender and slightly raised, the carapace is high, the chest pin is narrow, and the hind legs are often knife-shaped. Due to the long-term milking exercise, Kazakh horses h...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘武军刘玲玲方超何美升
Owner XINJIANG AGRI UNIV