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Kit containing ca8 gene for screening lactation traits of Kazakh horses and its application

A kit and lactating performance technology, applied in the identification field of animal genetic selection and breeding, can solve problems such as unseen research reports, achieve the effects of increasing economic income, protecting resources of fine varieties, and accelerating development

Active Publication Date: 2022-01-04
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been many studies on the functional genes of livestock milk production traits, but there are no relevant research reports on the CA8 gene as a candidate gene affecting the lactation traits of Kazakh horses

Method used

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  • Kit containing ca8 gene for screening lactation traits of Kazakh horses and its application
  • Kit containing ca8 gene for screening lactation traits of Kazakh horses and its application
  • Kit containing ca8 gene for screening lactation traits of Kazakh horses and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: The present invention is used to detect the kit of lactation traits of Kazakh horses

[0050] The invention provides a detection kit for detecting the lactation traits of the Kazakh horse of the CA8 gene, wherein the allele types of the CA8 gene include AA, AG and GG.

[0051] In the present invention, allelic types AA, AG and GG were detected from Kazakh horse plasma.

[0052] The present invention also provides a detection kit containing the CA8 gene for screening the lactation traits of Kazakh horses, comprising a box body 1, a label 12 is arranged on the outside of the box body 1, and the inside of the box body 1 is divided into 96-well plate areas by partitions 3 in turn 31. Tip area 32, blood collection tube area 33, DNA extraction reagent area 34, PCR amplification reagent area 35 and Marker area 36, ​​96-well plate area 31, tip area 32, blood collection tube area 33, DNA extraction reagent area 34 , PCR amplification reagent area 35 and Marker are...

Embodiment 2

[0064] Embodiment 2: The present invention is used to detect the kit of Kazakh horse lactation traits

[0065] The present invention provides a detection kit containing CA8 gene for screening the lactation traits of Kazakh horses. The box body 1 is in the shape of a cylinder, and a central column 4 is arranged in the middle of the box body 1. A shaft hole 42 is arranged at the top of the central column 4, and the cover is divided into two parts. 2. A rotating shaft 41 adapted to the shaft hole 42 is provided at the bottom, so that the sub-cover 2 can be rotated and placed on other sub-covers 2 after opening, without occupying space. The partition 3 and the sub-cover 2 make each area airtight and divided , will not interfere with each other.

[0066] In the present invention, the 96-well plate area 31, the tip area 32, the blood collection tube area 33, the DNA extraction reagent area 34, the PCR amplification reagent area 35 and the Marker area 36 are all fan-shaped areas, and...

Embodiment 3

[0071] Example 3: Application of a detection kit containing CA8 gene for improving lactation traits of Kazakh horses

[0072] The present invention provides the application of the detection kit containing CA8 gene for improving the lactation traits of Kazakh horses, which is embodied by the detection method and specifically includes the following steps:

[0073] (1) Extraction of horse genomic DNA:

[0074] Use a blood collection tube filled with sodium citrate anticoagulant to collect 5ml of Kazakh horse jugular venous blood in vivo, use the DNA extraction reagent, use the kit provided in the above example to extract genomic DNA, store at -20°C for later use, and extract the genome DAN gel electrophoresis, as attached Figure 4 shown.

[0075] (2) CA8 gene PCR amplification:

[0076] According to the designed primers, the kit provided in the above examples was used to specifically amplify the CA8 gene; the PCR reaction system was 2×Master Mix 2.5 μl, each of the upstream a...

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Abstract

The invention discloses a kit containing CA8 gene for screening the lactation traits of Kazakh horses and its application. The detection kit of the invention can be used to improve the lactation traits of Kazakh horses in breeding by detecting the AA, AG and GG alleles. The kit has six areas, including 96-well plate area, tip area, blood collection tube area, DNA extraction reagent area, PCR amplification reagent area and Marker area, all of which are fan-shaped areas. According to specific primers, the sample genomic DNA is used as a template Carry out PCR amplification, and carry out KASP typing after the PCR amplification product; It is of practical application value to improve the resources of excellent breeds, increase the economic income of farmers and herdsmen, and accelerate the development of dairy horses.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection, specifically, the invention relates to the technical field of a kit for screening lactation traits of Kazakh horses. Background technique [0002] Xinjiang's horse breeding industry has a long history and is famous for its rich production of fine horses. It is also one of the important horse production bases in my country. Xinjiang is rich in horse resources, and the number of horses in stock remains at about 890,000. There are a large number of horses and rich breed resources. Kazakh horses are herbivorous livestock. The Kazakh horse produced in Xinjiang is also a grassland horse breed. Its morphological characteristics are: the head is medium and large, delicate, and the ears are short. The neck is slender and slightly raised, the carapace is high, the chest pin is narrow, and the hind legs are often knife-shaped. Due to the long-term milking exercise, Kazakh horses h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘武军刘玲玲方超何美升
Owner XINJIANG AGRI UNIV