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A method for the rapid detection of paraquat based on a chemiluminescence resonance energy transfer sensor on a nano-gold surface

A technology of resonance energy transfer and chemiluminescence, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, to achieve the effects of high photon quantum yield, strong chemiluminescence intensity, and long luminescence time

Active Publication Date: 2020-12-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research hotspot in the field of optical sensing is to use nucleic acid as a molecular recognition element to construct biosensors. Since its research is still in its preliminary stage, it faces challenges such as improving the sensitivity of paraquat determination.

Method used

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  • A method for the rapid detection of paraquat based on a chemiluminescence resonance energy transfer sensor on a nano-gold surface

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Experimental program
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Effect test

Embodiment 1

[0029] A method for rapidly detecting paraquat based on a chemiluminescence resonance energy transfer sensor on a nano-gold surface, comprising the following steps:

[0030] S1. First, the gold nanoparticles and the horseradish peroxidase solution were incubated at 20° C. for 30 min to obtain horseradish peroxidase-gold nanoparticles (HRP-AuNPs) complexes.

[0031] S2. The horseradish peroxidase-nanogold complex obtained in step S1 and a series of paraquat standard solutions were mixed and incubated at 20°C for 30-35min to obtain a series of horseradish peroxidase-nanogold (HRP -AuNPs), paraquat-nanogold mixed solution; the concentrations of a series of paraquat standard solutions were 0.0ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 200ng / mL, 500ng / mL and 1000ng / mL . This reaction enriches more paraquat on the surface of gold nanometers, keeps HRP away from the surface of gold nanoparticles, thereby inhibiting the Luminol-H 2 o 2 - Chemiluminescence resonance energy transfer between ...

Embodiment 2

[0039] A method for rapidly detecting paraquat based on a chemiluminescence resonance energy transfer sensor on a nano-gold surface, comprising the following steps:

[0040] S1. First, the gold nanoparticles and the horseradish peroxidase solution were incubated at 15° C. for 35 minutes to obtain horseradish peroxidase-gold nanoparticles (HRP-AuNPs) complexes.

[0041] S2. The horseradish peroxidase-nanogold complex obtained in step S1 was mixed with a series of paraquat standard solutions at 15°C for 35min to obtain a series of horseradish peroxidase-nanogold (HRP-AuNPs ), paraquat-nanogold mixed solution; a series of paraquat standard solutions with concentrations of 0.0ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 200ng / mL, 500ng / mL and 1000ng / mL. This reaction enriches more paraquat on the surface of gold nanometers, keeps HRP away from the surface of gold nanoparticles, thereby inhibiting the Luminol-H 2 o 2 - Chemiluminescence resonance energy transfer between HRP-AuNPs.

[0042...

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Abstract

The invention provides a method for rapidly detecting paraquat on basis of chemiluminiscence resonance energy transfer sensors on surfaces of nanogold, and relates to the technical field of food detection. According to the method, horse radish peroxidase is enriched on the surfaces of nanogold, so that a necessary condition is provided for chemiluminiscence resonance energy transfer between luminol-hydrogen peroxide-horse radish peroxidase and the nanogold. A chemiluminescence reaction system of chemiluminiscence sensitizing liquid for horse radish peroxidase catalysis has unique optical properties such as high light quantum productivity and long luminescence time. Paraquat can effectively compete with the horse radish peroxidase to be enriched on the surfaces of the nanogold, so that thechemiluminiscence resonance energy transfer efficiency is reduced, the chemiluminiscence is recovered and stronger chemiluminiscence strength is detected. A microplate reader has a high-performance microplate detection system so that a unique advantage can be developed in batch rapid screening operation, so that the rapidness and high sensitivity of the detection area realized.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for rapidly detecting paraquat with a chemiluminescent resonance energy transfer sensor based on a nano-gold surface. Background technique [0002] Paraquat, chemical name 1,1-dimethyl-4,4-bipyridine cation, belongs to organic heterocyclic quaternary ammonium herbicides. It is a fast-killing herbicide with broad-spectrum quick-acting and contact-killing effects. It was once considered to be a pesticide that is harmless to the environment, and its output and consumption rank among the top in the world, and it has been promoted and used globally. However, in recent years, paraquat has been classified by the World Health Organization as a moderately toxic pesticide. It is extremely toxic and can cause serious damage to the heart, lungs, liver and kidneys. Once poisoned, there is no effective antidote, and the mortality rate of oral poisoning is over 90%. According ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 覃国新闫飞燕王静金茂俊劳水兵何洁莫仁甫周其峰杨玉霞王海军罗丽红
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI