An SNP Marker associated with the growth of grass carp in Hanjiang and Ruichang of the Yangtze river system

Abstract

The invention relates to an SNP Marker associated with the growth of grass carp in Hanjiang and Ruichang of the Yangtze river system. The SNP marker is located in the exon of the fst1 gene. At position 5 of that sequence shown in SEQ ID NO: 1, the base at this position is C or A, wherein the base at position 5 of other grass carp in the Yangtze river system is A, and the base at position 5 of Hanjiang and Ruichang grass carp is C, and the base is mutated from A to C through missense, thereby changing the composition of the amino acid sequence from lysine (Lys) to tryptophan (Thr). Experimentsshow that the growth rate and quality of grass carp in Hanjiang and Ruichang are significantly higher than those of other grass carps in the Yangtze river system, by detecting the SNP marker of grasscarp, Hanjiang grass carp and Ruichang grass carp with fast growth speed can be effectively selected under the same culture conditions. Further, the SNP marker of the invention can be used for molecular marker assisted breeding of grass carp, has positive significance for grass carp larval production, can greatly save breeding time, has low cost, high accuracy and speeds up grass carp breeding.

Description

technical field [0001] The invention relates to the technical field of SNP markers and applications thereof, in particular to a SNP marker related to the growth of Hanjiang and Ruichang grass carp in the Yangtze River system. Background technique [0002] Myostatin (Mstn) gene is one of the members of transforming growth factor β family, which can inhibit muscle differentiation and growth. Follistatin (Follistatin, Fst) gene is an activin-binding protein, which is expressed in all tissues, has the function of inhibiting mstn gene, and promotes muscle growth. At present, a large number of studies have been carried out on the function and regulation mechanism of mstn gene and fst gene in mammals. In terms of aquatic animals, the cDNA cloning and expression of mstn gene and fst gene of zebrafish, rainbow trout, tilapia, black-headed minnow, grass carp and other fish are mainly studied, and the functional research is relatively few. The polymorphism of mstn gene has been repor...

AI Technical Summary

Problems solved by technology

But, the research about grass carp fst gene single nucleotide polymorphism (Single nucleotide polymorphism, SNP) in the present invention is all not reported

Images