Method of separating D-fructose and D-psicose by using simulated moving bed

Abstract

The invention discloses a method of separating D-fructose and D-psicose by using a simulated moving bed. The method comprises the following steps of: step (1) converting D-psicose, to be specific, converting the D-fructose into the D-psicose by using immobilized D-psicose epimerase nanoparticles, thereby obtaining a mixed sugar solution; step (2) pretreating the mixed sugar solution, to be specific, carrying out decolorizing pretreatment on the mixed sugar solution; and step (3) separating by using the sequential simulated moving bed, to be specific, separating the pretreated mixed sugar solution according to the specific separation parameters of the sequential simulated moving bed to obtain the D-psicose solution and the D-fructose solution, wherein the purity of the D-psicose solution isgreater than or equal to 99%, the recovery rate of the D-psicose solution is greater than or equal to 95%, the purity of the D-fructose solution is greater than or equal to 99%, and the recovery rateof the D-psicose solution is greater than or equal to 90%. In summary, the method has the beneficial effects that the purity and recovery rate of the separated products are high, the sequential simulated moving bed is used for replacing a traditional simulated bed, and the number of chromatographic columns used and the production cost are reduced.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to a method for separating D-fructose and D-psicose by using a simulated moving bed. Background technique [0002] As an epimer of D-fructose, D-psicose is a kind of natural ketohexose which is relatively rare in nature, and belongs to a kind of rare sugar. Its sweetness intensity and type are very similar to D-fructose, and its calories are only 0.007kcal / g, so it is called a zero-calorie sweetener. At the same time, D-psicose also has good functional properties. D-psicose has only a small amount of absorption in the digestive tract and has a low degree of heat supply. It is used as a sweetener for adjuvant weight loss treatment; Oral administration of D-psicose can inhibit the activity of intestinal α-glucosidase, inhibit the rise of blood sugar after meals; it can inhibit the activity of liver fat synthase, and reduce fat deposition. [0003] Sim...

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Problems solved by technology

[0004] Although the market demand for allulose is increasing, the high price limits their market size and application

In recent years, the use of enzyme engineering technology to synthesize psicose from fructose by biocatalysis has become a method to reduce the production cost of psicose, but about half of the fructose has not been converted when the reaction is balanced, and it needs to be removed to produce High-purity allulose, but the properties of fructose and allulose are very similar, and it is difficult to separate the two. In the prior art, a single-column chromatographic separation device is used for separation, and the separated allulose and fructose The purity is not high, and there is no research result on the separation of fructose and allulose by sequential simulated moving bed chromatography

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