A kind of method utilizing simulated moving bed to separate d-fructose and d-psicose

Abstract

The invention discloses a method for separating D-fructose and D-psicose by using a simulated moving bed. The allulose epimerase nano-microspheres are converted into D-psicose to obtain a mixed sugar solution; (2) pretreatment of the mixed sugar solution: the mixed sugar solution is subjected to decolorization pretreatment; (3) sequential simulation Moving bed separation: The pretreated mixed sugar solution is separated according to the specific separation parameters of the sequential simulated moving bed. The purity of the finally separated D-psicose solution is ≧99%, the recovery rate is ≧95%, and the final separation The purity of the obtained D-fructose solution is ≧99%, and the recovery rate is ≧90%. In a word, the present invention has high purity and high recovery rate of separated products, and adopts sequential simulated moving bed instead of traditional simulated bed, which reduces the number of chromatographic columns used and production cost.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to a method for separating D-fructose and D-psicose by using a simulated moving bed. Background technique [0002] As an epimer of D-fructose, D-psicose is a kind of natural ketohexose which is relatively rare in nature, and belongs to a kind of rare sugar. Its sweetness intensity and type are very similar to D-fructose, and its calories are only 0.007kcal / g, so it is called a zero-calorie sweetener. At the same time, D-psicose also has good functional properties. D-psicose has only a small amount of absorption in the digestive tract and has a low degree of heat supply. It is used as a sweetener for adjuvant weight loss treatment; Oral administration of D-psicose can inhibit the activity of intestinal α-glucosidase, inhibit the rise of blood sugar after meals; it can inhibit the activity of liver fat synthase, and reduce fat deposition. [0003] Sim...

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Problems solved by technology

[0004] Although the market demand for allulose is increasing, the high price limits their market size and application

In recent years, the use of enzyme engineering technology to synthesize psicose from fructose by biocatalysis has become a method to reduce the production cost of psicose, but about half of the fructose has not been converted when the reaction is balanced, and it needs to be removed to produce High-purity allulose, but the properties of fructose and allulose are very similar, and it is difficult to separate the two. In the prior art, a single-column chromatographic separation device is used for separation, and the separated allulose and fructose The purity is not high, and there is no research result on the separation of fructose and allulose by sequential simulated moving bed chromatography

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