A kind of symbiotic anti-drought endophyte for promoting seed germination and its application
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A seed, germination rate technology, applied in application, seed and rhizome treatment, bacteria, etc.
Active Publication Date: 2022-06-14
XINJIANG NORMAL UNIVERSITY
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[0005]At present, most of the researches on plant seed germination are stresses of abiotic factors such as drought, salt, temperature, etc., while there are few studies on the influence of biotic factors, and most of them are symbiotic The research on microbial action focuses on the production of effective metabolic components of medicinal plants, and less attention is paid to desert plants
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Embodiment 1
[0044] Embodiment 1, the acquisition of bacillus AER314-4
[0045] 1. Isolation of strains
[0046] 37 strains of endophytic bacteria were isolated from the roots of Sophora chinensis.
[0047] 2. Screening of highly effective strains of endophytic bacteria for drought resistance and seed germination
[0048] Strains to be tested: 37 strains of endophytic bacteria isolated in step one.
[0049] 1. Pick a single colony of the strain to be tested, inoculate it in beef extract peptone liquid medium and cultivate it to the OD of the bacterial solution 600nm to around 1.0.
[0050] 2. Configure and prepare the culture medium as follows:
[0051] Experimental group: Spread double-layer sterile filter paper on a sterilized petri dish, mix 1.4mL of the bacterial solution obtained in step 1 and 2.8mL of 25% PEG6000 solution, and then evenly add to the filter paper;
[0052] Control group A: Spread double-layer sterile filter paper on a sterilized petri dish, mix 1.4 mL of beef ext...
Embodiment 2
[0082] Embodiment 2, the effect of Bacillus sp. AER314-4 on the substance metabolism in the process of seed germination
[0083] 1. The effect of Bacillus sp. AER314-4 on the activity of α-amylase in seeds during the germination process of Sophora chinensis
[0084] 1. Pick a single colony of the strain to be tested, inoculate it in beef extract peptone liquid medium and cultivate it to the OD of the bacterial solution 600nm When it reaches about 1.0, centrifuge the bacterial solution at 4°C and 8000r / min for 10min, collect the bacterial pellet and supernatant respectively, and resuspend the bacterial pellet with an equal volume of sterilized PBS buffer (pH=6.8) for later use.
[0085] 2. Soak the surface-sterilized Sophora japonica seeds in 98% concentrated sulfuric acid for 90 minutes (to rupture the seed coat), and then rinse with a large amount of sterile water until pH=7. Spread the treated seeds evenly in each group of medium in step 2 (25 grains per dish), weigh and re...
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Abstract
The invention discloses a symbiotic anti-drought endophyte for promoting seed germination and application thereof. The Bacillus mojavensis AER314‑4 protected by the present invention has a preservation number of CCTCC NO: M2018495. Experiments have confirmed that Bacillus sp. AER314‑4 can increase the germination rate of Sophora japonica seeds by 54% under the drought stress of 25% PEG 6000, and it is confirmed that it activates the amylase inside the seed to degrade the starch to produce soluble sugar and reduce the seed content. Osmotic potential to promote water absorption and germination. Bacillus has the characteristics of fast growth and strong stress resistance. It has no time and climate limitations, is safe for humans and animals, does not pollute the environment, and has no adverse effects on plants. The strain has the potential to be used as a desert plant seed to resist drought and promote germination, and it can be applied to desert plants to resist drought and promote seed germination to promote ecosystem restoration.
Description
technical field [0001] The invention relates to a symbiotic drought-resistant endophyte for promoting seed germination and application thereof. Background technique [0002] In order to better adapt to the environment and absorb nutrients, desert plants form a symbiotic relationship with microorganisms. The relatively stable environment in plant tissues is conducive to the survival of microorganisms and the continuation of the population, and the host plant obtains the adaptation function to the adversity from the metabolites of microorganisms or the regulation of genes. benefit from adversity. [0003] Ammodendron bifolium (Pall·) is a sand-growing deciduous shrub of the genus Ammodendron of the family Leguminosae. National Class II List of Endangered Plants. The study found that one of the endangering factors of Sophora chinensis is the difficulty of seed germination under drought conditions. As a native species in Xinjiang, Saccharia chinensis is of great value to the...
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