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A molecular marker and its application for rapid detection of high-yielding genes in Thiopyrum elongatum

A technology of Thinpyrum and gene, which is applied in the rapid detection of molecular markers and application fields of high-yield genes of Thinopyrum elongatum, can solve the problems of lack of molecular markers of high-yield genes, difficulty in phenotypic identification, and low breeding efficiency, so as to improve breeding efficiency, Reduce the cost of breeding and reduce the effect of the operation process

Active Publication Date: 2021-10-19
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, difficult to identify phenotypes, and have low breeding efficiency. Molecular marker-assisted selection breeding can effectively solve this problem
At present, there is no research report on molecular markers of this high-yielding gene

Method used

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  • A molecular marker and its application for rapid detection of high-yielding genes in Thiopyrum elongatum
  • A molecular marker and its application for rapid detection of high-yielding genes in Thiopyrum elongatum
  • A molecular marker and its application for rapid detection of high-yielding genes in Thiopyrum elongatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Creation of Near-Isogenic Lines of High Yield Genes of Wheat-Thinopyrum elongatum

[0060] In 2015, the hybrid combination (Liangxing 66×SNK064) was configured in the greenhouse of the Crop Research Institute of Shandong Academy of Agricultural Sciences, and obtained F 1 ; Spring 2016, will receive F 1 Backcross with Liangxing 66 and get BC 1 f 1 , and carry out molecular marker identification on the obtained plants, on the basis of molecular marker identification, the BC carrying target gene and exogenous fragment 1 f 1 Continue to backcross with Liangxing 66 and get BC 2 f 1 . In the summer and autumn of 2016, the artificial climate chamber continued to add generations, self-crossed twice, and obtained BC 2 f 3 , with the help of molecular markers and fluorescence in situ hybridization, a wheat-Thinopyrum elongatum 7el2L heterozygous line was obtained; in the winter of 2016 and the spring of 2017, the above heterozygous line continued to be selfed twic...

Embodiment 2

[0062] Example 2 Analysis of agronomic and yield-related traits of high-yielding near-isogenic lines of Echinopsis elongatum

[0063] Refer to the method of Tang et al. After the in situ hybridization chromosome preparation was dried naturally, 0.25 μg / mL propidium iodide was added dropwise and covered with a cover glass, and photographed under an Olympus BX51 fluorescence microscope.

[0064] The above-mentioned near-isogenic line materials (SAAS1001, SAAS1002 and SAAS1003) were planted in the Lingcheng Experimental Base in the winter of 2017, with an area of ​​1.5m×6.0m, and repeated 3 times. For the flowering stage and grain filling stage of wheat, the length and width of the flag leaf, the length of the ear, the number of grains per ear, the number of fertile spikelets, the number of sterile spikelets, etc. 6 traits were investigated and analyzed, and 30 panicles were investigated in each plot. After harvesting, use the Wanshen SC-G automatic seed testing and thousand-gr...

Embodiment 3

[0066] Example 3 Development of Tightly Linked Molecular Markers for High Yield Genes of Echinopsis elongatum

[0067] According to the sequence information of the published wheat genome (7DL end), SSR primers were designed using Primer 3.0, and wheat varieties Jimai 22, Liangxing 66, SAAS1001 and Jimai 22 mixed DNA, SAAS1002 and Liangxing 66 mixed DN, SAAS1003 and SAAS1001 Mixed DNA and wheat-Thinopyrum elongated near-isogenic lines SAAS1001, SAAS1002 and SAAS1003 were used as materials for PCR amplification to search for molecular markers closely linked to high-yielding genes.

[0068] In the PCR amplification reaction system, the volume of the PCR reaction system is 10.0 μL, including 10×Buffer buffer: 1.0 μL, 2.5 mM dNTP solution: 0.8 μL, Taq enzyme 0.1 μL, sterilized double distilled water: 4.1 μL, 50ng DNA Template 2.0 μL (Jimai 22, Liangxing 66, SAAS1001 and Jimai 22 mixed DNA, SAAS1002 and Liangxing 66 mixed DNA, SAAS1003 and SAAS1001 mixed DNA, and wheat-Ethiopia elon...

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Abstract

The invention belongs to the field of genetics, and provides a molecular marker for rapid detection of the high-yield gene of Echinopsis elongatum and its application. Through conventional hybridization, backcrossing, and additional generation techniques in greenhouses and artificial climate chambers, the 7el2L high-yield gene of Etypyronium elongatum is created. The gene line, combined with fluorescence in situ hybridization technology and analysis of agronomic and yield-related traits, has obtained molecular markers closely linked to it, which can be used for high-yield breeding of wheat and early molecular-assisted selection of yield-related traits to improve breeding efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and in particular relates to a molecular marker for rapidly detecting the high-yield gene of Echinopsis elongatum and its application. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in the world, its yield is second only to corn and rice, and it is the third largest food crop. The world's wheat planting area accounts for 17.0% of the world's total grain planting area (217 million hectares), and my country's annual wheat planting area is about 25 million hectares, accounting for 21.8% of the country's total grain planting area (113 million hectares) (FAO, 2012 ; National Bureau of Statistics of China, 2014). Wheat is one of the most important food crops because it feeds 40% of the world's population and provides 20% of human energy and protein supply. According to reports, by 2050, the total population of the world will reach more than ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 郭军刘建军李豪圣宋健民刘成曹新有刘爱峰赵振东
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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