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An anti-plant virus agent

A plant virus and pharmaceutical technology, applied in the field of pesticides, can solve the problems of difficult prevention and control of viral diseases and poor effect

Active Publication Date: 2020-07-10
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since plant viruses infect plants are fully dependent on the metabolism of the host to proliferate, it is difficult to specifically eliminate the virus without affecting the normal metabolic function of the host, so the prevention and treatment of viral diseases is extremely difficult
Existing antiviral drugs at home and abroad, such as Ningnanmycin, S-methylbenzo[1,2,3]thiadiazole-7-thiocarboxylate (BTH, syn.acibenzolar-S-methyl), etc. , mainly by inducing plants to produce system acquired resistance (System acquired resistance), the effect is poor and only preventive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The small molecule drug is, the structural formula is as follows:

[0029]

[0030] The small molecule agent was applied to the activity detection gel electrophoretic mobility shift assay (EMSA) of inhibiting the silencing suppressor P19, and the results were as follows: figure 1 shown. Mix P19 and the small molecule drug in the binding buffer, and fully react for 25 minutes at room temperature. Wherein the concentration of P19 is 2 μM, and the concentration of the small molecule drug is 2-200 ppm. Then add siRNA with a final concentration of 50 nM, mix thoroughly and continue to react at room temperature for 25 minutes. After electrophoresis and transfer to the membrane, the results were observed by color development. In the color development results, only siRNA can display the band, and it is in the lower part of the figure (indicated by the UnboundsiRNA arrow). The combination of silencing suppressor protein and siRNA will slow down the electrophoresis rate an...

Embodiment 2

[0033] The small molecule drug is, the structural formula is as follows:

[0034]

[0035] The small molecule agent was applied to the activity detection EMSA assay of inhibiting the suppressor of silencing 2b, and the results were as follows: figure 2 shown. Mix 2b and the small molecule drug in the binding buffer, and fully react at room temperature for 25 minutes. The concentration of 2b is 2 μM, and the concentration of the small molecule agent is 20-200 ppm. Then add siRNA with a final concentration of 50 nM, mix thoroughly and continue to react at room temperature for 25 minutes. After electrophoresis and transfer to the membrane, the results were observed by color development. In the color development results, only siRNA can display bands, which are located in the lower part of the figure (indicated by the arrow of Unbound siRNA). The combination of silencing suppressor protein and siRNA will slow down the electrophoresis rate and shorten the electrophoresis dis...

Embodiment 3

[0038] The control effect of the compound of formula (II) of the present invention on tomato bushy dwarf virus.

[0039] experimental method:

[0040] Select Nicotiana benthamiana at the 4-5 leaf stage with similar growth, fully grind 0.3g of fresh diseased leaves with obvious symptoms with 30mL double-distilled water, inoculate with 1% virus inoculum by friction with diatomaceous earth, and inoculate 2 leaves per plant. 2 hours, 1, and 3 days after inoculation, spray with 150ppm medicament aqueous solution. There were 20 plants in each treatment group.

[0041] The disease index was calculated on the 8th, 11th, and 14th days after inoculation, and the results are shown in Table 1.

[0042] a. Disease grading criteria:

[0043] Grade 0—asymptomatic.

[0044] Grade 1 - mild symptoms on inoculated leaves.

[0045] Grade 2—One to two systematic leaves with bright veins and deformation.

[0046] Grade 3—most of the upper leaves are deformed or the main lateral veins are necr...

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Abstract

The present invention relates to the field of agricultural chemicals. In particular, the present invention provides an agent against a plant virus, the agent comprising a small molecule agent shown in formula (I) or a pharmaceutically acceptable salt containing the chemical structure shown in formula (I), wherein R1 is selected from one of hydrogen, nitro, nitroso, a sulfo group, carboxyl, amino or azo, a halogen atom, C1-10 alkyl, C2-10 linear alkenyl, and C2-10 linear alkynyl. The small molecule agent of the present invention shows good inhibitory effect on viral silencing suppressors. As proved by pharmacodynamic experiments, the small molecule agent of the present invention has highly effective antiviral effect.

Description

technical field [0001] The invention relates to the field of pesticides, in particular to a pesticide against plant viruses. Background technique [0002] Plant virus disease is one of the important diseases of crops, known as "plant cancer", which brings billions of dollars of losses to global agricultural production every year. In recent years, especially for commercial crops, the harm of plant virus diseases has become increasingly serious. Such as tobacco virus disease, once the quality of tobacco leaves is reduced by at least 2-3 grades, or even completely loses economic value in severe cases, and because it is extremely difficult to control, the economic loss caused by it has far exceeded that of tobacco fungal disease, becoming a threat to tobacco production The largest category of disease. [0003] Since plant viruses infect plants are fully dependent on the metabolism of the host to proliferate, it is difficult to specifically eliminate the virus without affecting...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/04A01N43/90A01P1/00
CPCA01N43/90C07D493/04
Inventor 朱水芳胡帆邓宇芳雷荣李桂芬李新实
Owner CHINESE ACAD OF INSPECTION & QUARANTINE