Preparation method of PBMC cells
A cell and equipment technology, applied in the field of PBMC cell preparation, can solve problems such as complex structure
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Embodiment 1
[0085] Embodiment 1 Equipment assembly
[0086] This embodiment provides a device, which includes a sampling device, a collection device, a liquid replacement device, a centrifugal culture device, a liquid sensor device, a gas exchange device and a weighing component device;
[0087]The liquid sensor device includes a first liquid sensor and a second liquid sensor, wherein the first liquid sensor is connected to the sampling device, and the second liquid sensor is connected to the centrifugal culture device; the weighing component device includes a load cell, respectively Set on the sampling device, collection device, centrifugal culture device and fluid replacement device; wherein, the sampling device, centrifugal culture device, gas exchange device, fluid replacement device and collection device are sequentially connected; the sampling device includes a blood bag connection 1, blood clot filter 31 and first tube clamp 8; the blood bag connector 1, blood clot filter 31 and fi...
Embodiment 2
[0089] Example 2 Cell preparation
[0090] Cells were prepared as follows:
[0091] (1) Prepare a 100mL sample, connect consumables, and perform a self-test. The consumables include a blood sample bag 35, a lymphatic separation fluid bag 5, a collection bag 6, physiological saline bags 33-34, a culture medium bag 4, and a centrifuge bucket 29;
[0092] (2) Centrifugation is carried out in the centrifugal culture device by gradient centrifugation, and the liquid flow is adjusted through the cooperation of the liquid sensor device, the centrifugal culture device, the weighing component device and the valve device, and the cells in the sample are separated to the collection device;
[0093] (3) pump the physiological saline in the liquid replacement device into the centrifuge barrel 29, and then discharge it into the lymphatic separation liquid bag 5, repeating it several times;
[0094] The detailed operation steps are as follows:
[0095] Prepare samples:
[0096] Clamp all ...
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