Preparation method of PBMC cells

A cell and equipment technology, applied in the field of PBMC cell preparation, can solve problems such as complex structure

Inactive Publication Date: 2019-02-01
EUREKA BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the structure of the above-mentioned prio

Method used

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  • Preparation method of PBMC cells
  • Preparation method of PBMC cells
  • Preparation method of PBMC cells

Examples

Experimental program
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Effect test

Embodiment 1

[0085] Embodiment 1 Equipment assembly

[0086] This embodiment provides a device, which includes a sampling device, a collection device, a liquid replacement device, a centrifugal culture device, a liquid sensor device, a gas exchange device and a weighing component device;

[0087]The liquid sensor device includes a first liquid sensor and a second liquid sensor, wherein the first liquid sensor is connected to the sampling device, and the second liquid sensor is connected to the centrifugal culture device; the weighing component device includes a load cell, respectively Set on the sampling device, collection device, centrifugal culture device and fluid replacement device; wherein, the sampling device, centrifugal culture device, gas exchange device, fluid replacement device and collection device are sequentially connected; the sampling device includes a blood bag connection 1, blood clot filter 31 and first tube clamp 8; the blood bag connector 1, blood clot filter 31 and fi...

Embodiment 2

[0089] Example 2 Cell preparation

[0090] Cells were prepared as follows:

[0091] (1) Prepare a 100mL sample, connect consumables, and perform a self-test. The consumables include a blood sample bag 35, a lymphatic separation fluid bag 5, a collection bag 6, physiological saline bags 33-34, a culture medium bag 4, and a centrifuge bucket 29;

[0092] (2) Centrifugation is carried out in the centrifugal culture device by gradient centrifugation, and the liquid flow is adjusted through the cooperation of the liquid sensor device, the centrifugal culture device, the weighing component device and the valve device, and the cells in the sample are separated to the collection device;

[0093] (3) pump the physiological saline in the liquid replacement device into the centrifuge barrel 29, and then discharge it into the lymphatic separation liquid bag 5, repeating it several times;

[0094] The detailed operation steps are as follows:

[0095] Prepare samples:

[0096] Clamp all ...

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Abstract

The invention provides a preparation method of PBMC cells. According to the method, equipment for preparing cells is adopted, a weighing sensor is used to replace optical grating, and an air inlet unit and a liquid sensor are additionally arranged. By optimizing the collocation relationship between various devices in the overall equipment and simplifying operation process of the equipment and withthe cooperation of various devices and various steps, the production cost is reduced, the cell separation process is more intelligent and automatic, the operation is simpler, cell pollution is reduced, and the success rate of PBMC cell preparation is raised. The method of the invention has a broad application prospect and great market value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for preparing PBMC cells. Background technique [0002] Cell therapy is a method of separating and extracting some of the cells from human or animal tissues and blood, and directly infusing them back into humans or animals, or by screening, genetically modifying, inducing differentiation, and culturing and expanding some of the extracted cells. A form of treatment that is infused back into humans or animals. Cellular immunotherapy is a rapidly developed cell therapy for various diseases in recent years. It achieves the goal of curing diseases by reinfusing in vitro screened, modified, induced, and expanded lymphocytes in tissues or blood. Common applications include preparing dendritic cells (DC), T cells, and NK cells to treat cancer; preparing Treg cells to treat autoimmune diseases and treating various diseases such as infertility, allergies, and viral infections. ...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/12C12M1/00C12N5/078
CPCC12M41/12C12M41/40C12M47/04C12N5/0634
Inventor 马墨薛博夫陈林雄郑伟武
Owner EUREKA BIOTECH LTD
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